Human NEU3 ELISA Kit (V2LY-0626-LY3644)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.017 ng/mL
Detection Range
0.05-15 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
48.3 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
neuraminidase 3
Function
Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Displays high catalytic efficiency for gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3 (PubMed:11298736, PubMed:15847605, PubMed:10861246, PubMed:20511247, PubMed:28646141, PubMed:10405317, PubMed:12011038).

Plays a role in the regulation of transmembrane signaling through the modulation of ganglioside content of the lipid bilayer and by direct interaction with signaling receptors, such as EGFR (PubMed:17334392, PubMed:25922362).

Desialylates EGFR and activates downstream signaling in proliferating cells (PubMed:25922362).

Contributes to clathrin-mediated endocytosis by regulating sorting of endocytosed receptors to early and recycling endosomes (PubMed:26251452).
Biological Process
Carbohydrate metabolic process Source: MGI
Ganglioside catabolic process Source: UniProtKB
Glycosphingolipid metabolic process Source: Reactome
Negative regulation of clathrin-dependent endocytosis Source: UniProtKB
Oligosaccharide catabolic process Source: UniProtKB
Positive regulation of epidermal growth factor receptor signaling pathway Source: UniProtKB
Cellular Location
Endosome
Early endosome membrane
Recycling endosome membrane
Lysosome
Lysosome membrane
Plasma membrane
Cell membrane
Other locations
caveola
Note: Associates with the external leaflet of the plasma membrane (By similarity). S-acylated NEU3 likely spans the lipid bilayer with a portion of C-terminus exposed to the cytosol and the catalytic region facing the extracellular space (PubMed:28646141).
PTM
Palmitoylated; may regulate intracellular trafficking and anchorage to plasma membrane and endomembranes.

Delaveris, C. S., Wang, C. L., Riley, N. M., Li, S., Kulkarni, R. U., & Bertozzi, C. R. (2023). Microglia Mediate Contact-Independent Neuronal Network Remodeling via Secreted Neuraminidase-3 Associated with Extracellular Vesicles. ACS Central Science, 9(11), 2108-2114.

Kirolos, S. A., & Gomer, R. H. (2022). The extracellular sialidase NEU3 induces neutrophil priming. bioRxiv, 2022-02.

Kirolos, S. A., Pilling, D., & Gomer, R. H. (2022). The extracellular sialidase NEU3 primes neutrophils. Journal of Leukocyte Biology, 112(6), 1399-1411.

Miyagi, T., & Yamamoto, K. (2022). Sialidase NEU3 and its pathological significance. Glycoconjugate Journal, 39(5), 677-683.

Tran, H. T. T., Li, C., Chakraberty, R., & Cairo, C. W. (2022). NEU1 and NEU3 enzymes alter CD22 organization on B cells. Biophysical Reports, 2(3).

Yang, W. H., Westman, J. S., Heithoff, D. M., Sperandio, M., Cho, J. W., Mahan, M. J., & Marth, J. D. (2021). Neu3 neuraminidase induction triggers intestinal inflammation and colitis in a model of recurrent human food-poisoning. Proceedings of the National Academy of Sciences, 118(29), e2100937118.

Kim, C. H., & Kim, C. H. (2020). Membrane Ganglioside-Specific Neuraminidase 3 (NEU3) Regulates GM3 Signaling. GM3 Signaling, 49-54.

Howlader, M. A., Li, C., Zou, C., Chakraberty, R., Ebesoh, N., & Cairo, C. W. (2019). Neuraminidase-3 is a negative regulator of LFA-1 adhesion. Frontiers in Chemistry, 7, 791.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Neu3 ELISA Kit (CAT#: V2LY-0626-LY1453)

Related Products

Mouse Neu3 ELISA Kit (CAT#: V2LY-0626-LY1453)

Mouse Anti-NEU3 Recombinant Antibody (11B) (CAT#: CBMAB-N2027-WJ)

Isotype control

For research use only. Not intended for any clinical use.

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