Mouse Anti-NEU3 Recombinant Antibody (11B) (CBMAB-N2027-WJ)

This product is a Mouse antibody that recognizes NEU3. The antibody 11B can be used for immunoassay techniques such as: WB.
See all NEU3 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

11B

Antibody Isotype

IgG1, κ

Application

Basic Information

Specificity

Human

Antibody Isotype

IgG1, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2, 50% glycerol

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

neuraminidase 3

Introduction

This gene product belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. It is localized in the plasma membrane, and its activity is specific for gangliosides. It may play a role in modulating the ganglioside content of the lipid bilayer. [provided by RefSeq, Jul 2008]

Entrez Gene ID

10825

UniProt ID

Q9UQ49

Alternative Names

Neuraminidase 3; Sialidase 3 (Membrane Sialidase); N-Acetyl-Alpha-Neuraminidase 3; Ganglioside Sialidase; Membrane Sialidase; EC 3.2.1.18; Neuraminidase 3, Membrane Sialidase; Sialidase-3; SIAL3;

Function

Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Displays high catalytic efficiency for gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3 (PubMed:11298736, PubMed:15847605, PubMed:10861246, PubMed:20511247, PubMed:28646141, PubMed:10405317, PubMed:12011038).

Plays a role in the regulation of transmembrane signaling through the modulation of ganglioside content of the lipid bilayer and by direct interaction with signaling receptors, such as EGFR (PubMed:17334392, PubMed:25922362).

Desialylates EGFR and activates downstream signaling in proliferating cells (PubMed:25922362).

Contributes to clathrin-mediated endocytosis by regulating sorting of endocytosed receptors to early and recycling endosomes (PubMed:26251452).

Biological Process

Carbohydrate metabolic process Source: MGI
Ganglioside catabolic process Source: UniProtKB
Glycosphingolipid metabolic process Source: Reactome
Negative regulation of clathrin-dependent endocytosis Source: UniProtKB
Oligosaccharide catabolic process Source: UniProtKB
Positive regulation of epidermal growth factor receptor signaling pathway Source: UniProtKB

Cellular Location

Endosome
Early endosome membrane
Recycling endosome membrane
Lysosome
Lysosome membrane
Plasma membrane
Cell membrane
Other locations
caveola
Note: Associates with the external leaflet of the plasma membrane (By similarity). S-acylated NEU3 likely spans the lipid bilayer with a portion of C-terminus exposed to the cytosol and the catalytic region facing the extracellular space (PubMed:28646141).

PTM

Palmitoylated; may regulate intracellular trafficking and anchorage to plasma membrane and endomembranes.

References

Delaveris, C. S., Wang, C. L., Riley, N. M., Li, S., Kulkarni, R. U., & Bertozzi, C. R. (2023). Microglia Mediate Contact-Independent Neuronal Network Remodeling via Secreted Neuraminidase-3 Associated with Extracellular Vesicles. ACS Central Science, 9(11), 2108-2114.

Kirolos, S. A., & Gomer, R. H. (2022). The extracellular sialidase NEU3 induces neutrophil priming. bioRxiv, 2022-02.

Kirolos, S. A., Pilling, D., & Gomer, R. H. (2022). The extracellular sialidase NEU3 primes neutrophils. Journal of Leukocyte Biology, 112(6), 1399-1411.

Miyagi, T., & Yamamoto, K. (2022). Sialidase NEU3 and its pathological significance. Glycoconjugate Journal, 39(5), 677-683.

Tran, H. T. T., Li, C., Chakraberty, R., & Cairo, C. W. (2022). NEU1 and NEU3 enzymes alter CD22 organization on B cells. Biophysical Reports, 2(3).

Yang, W. H., Westman, J. S., Heithoff, D. M., Sperandio, M., Cho, J. W., Mahan, M. J., & Marth, J. D. (2021). Neu3 neuraminidase induction triggers intestinal inflammation and colitis in a model of recurrent human food-poisoning. Proceedings of the National Academy of Sciences, 118(29), e2100937118.

Kim, C. H., & Kim, C. H. (2020). Membrane Ganglioside-Specific Neuraminidase 3 (NEU3) Regulates GM3 Signaling. GM3 Signaling, 49-54.

Howlader, M. A., Li, C., Zou, C., Chakraberty, R., Ebesoh, N., & Cairo, C. W. (2019). Neuraminidase-3 is a negative regulator of LFA-1 adhesion. Frontiers in Chemistry, 7, 791.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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