Human NLRX1 ELISA Kit (V2LY-0626-LY4959)

Name: Human NLRX1 ELISA Kit
SKU: V2LY-0626-LY4959
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0075 ng/mL

Detection Range

0.015-3 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

107.6 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

NLR Family Member X1

Function

Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010).
Instead, promotes autophagy by interacting with TUFM and subsequently recruiting the autophagy-related proteins ATG5 and ATG12 (PubMed:22749352).
Regulates also MAVS-dependent NLRP3 inflammasome activation to attenuate apoptosis (PubMed:27393910).
Has no inhibitory function on NF-kappa-B signaling pathway, but enhances NF-kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313).

Biological Process

Innate immune responseIEA:UniProtKB-KW
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of inflammatory responseIEA:Ensembl
Negative regulation of innate immune responseIEA:Ensembl
Negative regulation of interferon-beta productionIEA:Ensembl
Negative regulation of interleukin-6 productionIEA:Ensembl
Negative regulation of RIG-I signaling pathwayIEA:Ensembl

Cellular Location

Mitochondrion outer membrane

Bi, P. Y., Killackey, S. A., Schweizer, L., & Girardin, S. E. (2023). NLRX1: versatile functions of a mitochondrial NLR protein that controls mitophagy. Biomedical Journal, 100635.

Pickering, R. J., & Booty, L. M. (2021). NLR in eXile: Emerging roles of NLRX1 in immunity and human disease. Immunology, 162(3), 268-280.

Fekete, T., Bencze, D., Bíró, E., Benkő, S., & Pázmándi, K. (2021). Focusing on the cell type specific regulatory actions of NLRX1. International journal of molecular sciences, 22(3), 1316.

Kastelberg, B., Tubau-Juni, N., Ayubi, T., Leung, A., Leber, A., Hontecillas, R., ... & Kale, S. D. (2020). NLRX1 is a key regulator of immune signaling during invasive pulmonary aspergillosis. PLoS Pathogens, 16(9), e1008854.

Luo, X., Donnelly, C. R., Gong, W., Heath, B. R., Hao, Y., Donnelly, L. A., ... & Lei, Y. L. (2020). HPV16 drives cancer immune escape via NLRX1-mediated degradation of STING. The Journal of clinical investigation, 130(4), 1635-1652.

Zhang, H., Xiao, Y., Nederlof, R., Bakker, D., Zhang, P., Girardin, S. E., ... & Zuurbier, C. J. (2020). NLRX1 deletion increases ischemia-reperfusion damage and activates glucose metabolism in mouse heart. Frontiers in immunology, 11, 591815.

Chu, X., Wu, S., & Raju, R. (2019). NLRX1 regulation following acute mitochondrial injury. Frontiers in immunology, 10, 2431.

Nagai-Singer, M. A., Morrison, H. A., & Allen, I. C. (2019). NLRX1 is a multifaceted and enigmatic regulator of immune system function. Frontiers in Immunology, 10, 2419.

Ma, D., Zhao, Y., She, J., Zhu, Y., Zhao, Y., Liu, L., & Zhang, Y. (2019). NLRX1 alleviates lipopolysaccharide-induced apoptosis and inflammation in chondrocytes by suppressing the activation of NF-κB signaling. International Immunopharmacology, 71, 7-13.

Gharagozloo, M., Mahmoud, S., Simard, C., Yamamoto, K., Bobbala, D., Ilangumaran, S., ... & Gris, D. (2019). NLRX1 inhibits the early stages of CNS inflammation and prevents the onset of spontaneous autoimmunity. PLoS biology, 17(9), e3000451.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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