Human OSBPL3 ELISA Kit (V2LY-0626-LY3778)

Name: Human OSBPL3 ELISA Kit
SKU: V2LY-0626-LY3778
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.053 ng/mL

Detection Range

0.1-38 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

101.2 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Oxysterol Binding Protein Like 3

Function

Phosphoinositide-binding protein which associates with both cell and endoplasmic reticulum (ER) membranes (PubMed:16143324).
Can bind to the ER membrane protein VAPA and recruit VAPA to plasma membrane sites, thus linking these intracellular compartments (PubMed:25447204).
The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface (PubMed:18270267, PubMed:25447204).
With VAPA, may regulate ER morphology (PubMed:16143324).
Has a role in regulation of the actin cytoskeleton, cell polarity and cell adhesion (PubMed:18270267).
Binds to phosphoinositides with preference for PI(3,4)P2 and PI(3,4,5)P3 (PubMed:16143324).
Also binds 25-hydroxycholesterol and cholesterol (PubMed:17428193).

Biological Process

Bile acid biosynthetic processTAS:Reactome

Cellular Location

Endoplasmic reticulum membrane
Cytoplasm, cytosol
Cell membrane
Cell projection, filopodium tip
Nucleus membrane
Co-localizes with OSBPL6 at contact sites between the plasma membrane and the endoplasmic reticulum.

PTM

Phosphorylation is enhanced in vitro by phorbol-12-myristate-13-acetate (PMA), forskolin and calcium ionophore A23187 (PubMed:25447204).
Phosphorylation seems to be stimulated in conditions of low cell-cell (or cell-matrix) adhesion (PubMed:18270267).

Shan, S., Wang, X., Qian, L., Wang, C., & Zhao, S. (2024). ENST00000534735 inhibits proliferation and migration, promotes apoptosis and pyroptosis of endometrial cancer via OSBPL3 through APMK/SIRT1/NF-κB pathway. Heliyon, 10(4).

Xiang, L., & Su, Z. (2024). High-intensity interval training can inhibit of PPARγ/OSBPL3 pathway through epigenetic alterations in Non-Alcoholic Fatty Liver Disease. International Neurourology Journal, 28(1), 349-355.

Wang, C., He, Y., He, Y., Liang, W., Zhou, C., Wu, M., ... & Cao, J. (2023). Prognostic and biological function value of OSBPL3 in colorectal cancer analyzed by multi-omic data analysis. BMC gastroenterology, 23(1), 270.

Su, Y., Xue, C., Gu, X., Sun, Y., Zhang, R., & Li, L. (2023). Integrated bioinformatics analysis reveals the function and prognostic value of OSBPL3 in hepatocellular carcinoma. Heliyon, 9(6).

Zhang, M., Meng, L., Zhang, Z., Wu, J., Chen, X., Wang, Y., & He, J. (2022). The relationships of OSBPL3 expression with KI-67 expression and KRAS mutations in CRC: implications for diagnosis and prognosis. BMC Medical Genomics, 15(1), 259.

Zhang, M., Meng, L., Zhang, Z., Wu, J., Chen, X., & He, J. (2022). The respective relations of expression of OSBPL3 with Ki-67 expression and KRAS mutation in CRC for diagnosis and prognosis.

Hu, Q., Masuda, T., Koike, K., Sato, K., Tobo, T., Kuramitsu, S., ... & Mimori, K. (2021). Oxysterol binding protein-like 3 (OSBPL3) is a novel driver gene that promotes tumor growth in part through R-Ras/Akt signaling in gastric cancer. Scientific reports, 11(1), 19178.

Hao, N., Zhou, Y., Li, Y., Zhang, H., Wang, B., Liu, X., ... & Tang, X. (2021). Clinical value and potential mechanisms of oxysterol-binding protein like 3 (OSBPL3) in human tumors. Frontiers in Molecular Biosciences, 8, 739978.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-OSBPL3 Recombinant Antibody (CBXO-0348) (CAT#: CBMAB-O0593-CQ)

Isotype control

For research use only. Not intended for any clinical use.

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