Human P4HA2 ELISA Kit (V2LY-0626-LY5773)

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Tested Data
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.008 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
60.9 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Prolyl 4-Hydroxylase Subunit Alpha 2
Function
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Biological Process
Peptidyl-proline hydroxylation to 4-hydroxy-L-prolineManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Endoplasmic reticulum lumen
Involvement in disease
Myopia 25, autosomal dominant (MYP25):
A refractive error of the eye, in which parallel rays from a distant object come to focus in front of the retina, vision being better for near objects than for far.

Zhang, J., Lyu, Z., Li, B., You, Z., Cui, N., Li, Y., ... & Ma, X. (2023). P4HA2 induces hepatic ductular reaction and biliary fibrosis in chronic cholestatic liver diseases. Hepatology, 78(1), 10-25.

Tolonen, J. P., Salo, A. M., Finnilä, M., Aro, E., Karjalainen, E., Ronkainen, V. P., ... & Myllyharju, J. (2022). Reduced Bone Mass in Collagen Prolyl 4 Hydroxylase P4ha1+/−; P4ha2−/− Compound Mutant Mice. Journal of Bone and Mineral Research Plus, 6(6), e10630.

Shang, L., Jiang, W., Zhang, J., & Wu, W. (2022). P4HA2 promotes occurrence and progression of liver cancer by regulating the PI3K/Akt/mTOR signaling pathway. Nan Fang yi ke da xue xue bao= Journal of Southern Medical University, 42(5), 665-672.

Aggarwal, V., Sahoo, S., Donnenberg, V. S., Chakraborty, P., Jolly, M. K., & Sant, S. (2022). P4HA2: A link between tumor-intrinsic hypoxia, partial EMT and collective migration. Advances in cancer biology-metastasis, 5, 100057.

Wu, Y., Zhang, X., Wang, J., Ji, R., Zhang, L., Qin, J., ... & Zhang, X. (2021). P4HA2 promotes cell proliferation and migration in glioblastoma. Oncology Letters, 22(2), 1-11.

Zhu, M., Peng, R., Liang, X., Lan, Z., Tang, M., Hou, P., ... & Wang, G. (2021). P4HA2-induced prolyl hydroxylation suppresses YAP1-mediated prostate cancer cell migration, invasion, and metastasis. Oncogene, 40(41), 6049-6056.

Lin, J., Jiang, L., Wang, X., Wei, W., Song, C., Cui, Y., ... & Qiu, G. (2021). P4HA2 promotes epithelial-to-mesenchymal transition and glioma malignancy through the collagen-dependent PI3K/AKT pathway. Journal of oncology, 2021, 1-14.

Cao, Y., Han, Q., Li, J., Jia, Y., Zhang, R., & Shi, H. (2020). P4HA2 contributes to cervical cancer progression via inducing epithelial-mesenchymal transition. Journal of Cancer, 11(10), 2788.

Zhang, T., Piao, H. Y., Guo, S., Zhao, Y., Wang, Y., Zheng, Z. C., & Zhang, J. (2020). LncRNA PCGEM1 enhances metastasis and gastric cancer invasion through targeting of miR-129-5p to regulate P4HA2 expression. Experimental and Molecular Pathology, 116, 104487.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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