Human Recombinant AAK1, Active protein, GST Tag (V2LY-0526-LY1877)

Name: Human Recombinant AAK1, Active protein, GST Tag
SKU: V2LY-0526-LY1877
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant AAK1, Active protein, GST Tag consist of Amino Acid: 1-465 and predicts a molecular mass of 78 kDa.

Molecule Mass

78 kDa

Sequence

Amino Acid: 1-465

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

AP2 Associated Kinase 1

Function

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Preferentially, may phosphorylate substrates on threonine residues. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity. (Microbial infection) By regulating clathrin-mediated endocytosis, AAK1 plays a role in the entry of hepatitis C virus as well as for the lifecycle of other viruses such as Ebola and Dengue.

Biological Process

Endocytosis
Membrane organization
Positive regulation of Notch signaling pathway
Protein autophosphorylation
Protein phosphorylation
Protein stabilization
Regulation of clathrin-dependent endocytosis
Regulation of protein localization

Cellular Location

Cell membrane; Clathrin-coated pit; Presynapse. Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells.

PTM

Autophosphorylated.

Martinez-Gualda, B., Schols, D., & De Jonghe, S. (2021). A patent review of adaptor associated kinase 1 (AAK1) inhibitors (2013-present). Expert Opinion on Therapeutic Patents, (just-accepted).

Einav, S., De Jonghe, S., Brannan, J., & Andino, R. (2019). Selective AAK1 and GAK Inhibitors for Combating Dengue and Other Emerging Viral Infections. Stanford University School of Medicine Stanford United States.

Agajanian, M. J., Walker, M. P., Axtman, A. D., Ruela-de-Sousa, R. R., Serafin, D. S., Rabinowitz, A. D., ... & Major, M. B. (2019). WNT activates the AAK1 kinase to promote clathrin-mediated endocytosis of LRP6 and establish a negative feedback loop. Cell reports, 26(1), 79-93.

Wells, C., Counago, R. M., Limas, J. C., Almeida, T. L., Cook, J. G., Drewry, D. H., ... & Axtman, A. D. (2019). SGC-AAK1-1: a chemical probe targeting AAK1 and BMP2K. ACS medicinal chemistry letters, 11(3), 340-345.

Fu, X., Ke, M., Yu, W., Wang, X., Xiao, Q., Gu, M., & Lü, Y. (2018). Periodic variation of aak1 in an aβ 1–42-induced mouse model of alzheimer’s disease. Journal of Molecular Neuroscience, 65(2), 179-189.

Dorosky, D., Prugar, L. I., Pu, S., O’Brien, C., Bakken, R., De Jonghe, S., ... & Einav, S. (2018). AAK1 and GAK inhibitors demonstrate activity against Filoviruses.

Agajanian, M. J., Walker, M. P., Axtman, A. D., Ruela-de-Sousa, R. R., Rabinowitz, A. D., Graham, D. M., ... & Major, M. B. (2018). AAK1 inhibits WNT signaling by promoting clathrin-mediated endocytosis of LRP6. bioRxiv, 258632.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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