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Mouse Anti-AAK1 Recombinant Antibody (V2-178859) (CBMAB-A0077-YC)

Provided herein is a Mouse monoclonal antibody against Human AP2 Associated Kinase 1. The antibody can be used for immunoassay techniques, such as ELISA, IF, WB.
See all AAK1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-178859
Antibody Isotype
IgG2a, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
AAK1 (AAH02695, 315-415 aa) partial recombinant protein with GST tag. Immunogen sequence: LKKECPIPNV QNSPIPAKLP EPVKASEAAA KKTQPKARLT DPIPTTETSI APRQRPKAGQ TQPNPGILPI QPALTPRKRA TVQPPPQAAG SSNQPGLLAS V
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 315-415

Target

Full Name
AP2 Associated Kinase 1
Introduction
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfami
Entrez Gene ID
22848
UniProt ID
Q2M2I8
Alternative Names
AP2 Associated Kinase 1; EC 2.7.11.1; Adaptor-Associated Kinase 1; KIAA1048;
Function
Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Preferentially, may phosphorylate substrates on threonine residues. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity. (Microbial infection) By regulating clathrin-mediated endocytosis, AAK1 plays a role in the entry of hepatitis C virus as well as for the lifecycle of other viruses such as Ebola and Dengue.
Biological Process
Endocytosis
Membrane organization
Positive regulation of Notch signaling pathway
Protein autophosphorylation
Protein phosphorylation
Protein stabilization
Regulation of clathrin-dependent endocytosis
Regulation of protein localization
Cellular Location
Cell membrane; Clathrin-coated pit; Presynapse. Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells.
PTM
Autophosphorylated.

Martinez-Gualda, B., Schols, D., & De Jonghe, S. (2021). A patent review of adaptor associated kinase 1 (AAK1) inhibitors (2013-present). Expert Opinion on Therapeutic Patents, (just-accepted).

Einav, S., De Jonghe, S., Brannan, J., & Andino, R. (2019). Selective AAK1 and GAK Inhibitors for Combating Dengue and Other Emerging Viral Infections. Stanford University School of Medicine Stanford United States.

Agajanian, M. J., Walker, M. P., Axtman, A. D., Ruela-de-Sousa, R. R., Serafin, D. S., Rabinowitz, A. D., ... & Major, M. B. (2019). WNT activates the AAK1 kinase to promote clathrin-mediated endocytosis of LRP6 and establish a negative feedback loop. Cell reports, 26(1), 79-93.

Wells, C., Counago, R. M., Limas, J. C., Almeida, T. L., Cook, J. G., Drewry, D. H., ... & Axtman, A. D. (2019). SGC-AAK1-1: a chemical probe targeting AAK1 and BMP2K. ACS medicinal chemistry letters, 11(3), 340-345.

Fu, X., Ke, M., Yu, W., Wang, X., Xiao, Q., Gu, M., & Lü, Y. (2018). Periodic variation of aak1 in an aβ 1–42-induced mouse model of alzheimer’s disease. Journal of Molecular Neuroscience, 65(2), 179-189.

Dorosky, D., Prugar, L. I., Pu, S., O’Brien, C., Bakken, R., De Jonghe, S., ... & Einav, S. (2018). AAK1 and GAK inhibitors demonstrate activity against Filoviruses.

Agajanian, M. J., Walker, M. P., Axtman, A. D., Ruela-de-Sousa, R. R., Rabinowitz, A. D., Graham, D. M., ... & Major, M. B. (2018). AAK1 inhibits WNT signaling by promoting clathrin-mediated endocytosis of LRP6. bioRxiv, 258632.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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