Human Recombinant AGER protein, Biotin Conjugated, hFc & AVI Tag (V2LY-0526-LY6449)

Name: Human Recombinant AGER protein, Biotin Conjugated, hFc & AVI Tag
SKU: V2LY-0526-LY6449
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc & AVI Tag

Protein Construction

This product is Human Recombinant AGER protein, Biotin Conjugated, hFc & AVI Tag consist of Amino Acid: 1-344 and predicts a molecular mass of 62.71 kDa.

Molecule Mass

62.71 kDa

Verified

HPLC

Conjugates

Biotin

Sequence

Amino Acid: 1-344

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Advanced Glycosylation End-Product Specific Receptor

Function

Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides.

Biological Process

Astrocyte activation
Cell surface receptor signaling pathway
Cellular response to amyloid-beta
Glucose mediated signaling pathway
Induction of positive chemotaxis
Inflammatory response
Innate immune response
Learning or memory
Microglial cell activation
Modulation of age-related behavioral decline
Negative regulation of blood circulation
Negative regulation of connective tissue replacement involved in inflammatory response wound healing
Negative regulation of interleukin-10 production
Negative regulation of long-term synaptic depression
Negative regulation of long-term synaptic potentiation
Neuron projection development
Positive regulation of activated T cell proliferation
Positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process
Positive regulation of chemokine production
Positive regulation of dendritic cell differentiation
Positive regulation of endothelin production
Positive regulation of ERK1 and ERK2 cascade
Positive regulation of heterotypic cell-cell adhesion
Positive regulation of interleukin-12 production
Positive regulation of interleukin-1 beta production
Positive regulation of interleukin-6 production
Positive regulation of JNK cascade
Positive regulation of JUN kinase activity
Positive regulation of monocyte chemotactic protein-1 production
Positive regulation of monocyte extravasation
Positive regulation of NF-kappaB transcription factor activity
Positive regulation of NIK/NF-kappaB signaling
Positive regulation of p38MAPK cascade
Positive regulation of protein phosphorylation
Positive regulation of tumor necrosis factor production
Protein localization to membrane
Regulation of CD4-positive, alpha-beta T cell activation
Regulation of DNA binding
Regulation of inflammatory response
Regulation of long-term synaptic potentiation
Regulation of NIK/NF-kappaB signaling
Regulation of p38MAPK cascade
Regulation of spontaneous synaptic transmission
Regulation of synaptic plasticity
Regulation of T cell mediated cytotoxicity
Response to amyloid-beta
Response to hypoxia
Response to wounding
Transcytosis
Transport across blood-brain barrier

Cellular Location

Isoform 1: Cell membrane
Isoform 2: Secreted
Isoform 10: Cell membrane

Topology

Extracellular: 23-342 aa
Helical: 343-363 aa
Cytoplasmic: 364-404 aa

Li, S., Hu, D., Hu, S., Sun, Y., Zhang, Y., Li, H., ... & Wang, D. W. (2020). Association of rs2070600 in advanced glycosylation end‐product specific receptor with prognosis of heart failure. ESC heart failure, 7(6), 3561-3572.

Mamoor, S. (2020). The receptor for advanced glycosylation end products, RAGE (AGER) is differentially expressed in non-small cell lung cancer and associates with patient survival.

Xue, X. X., Lei, H. Q., Zhao, L., Wang, X. Y., Wang, Z., Xie, L. Y., & Jia, J. H. (2020). MiR-185-3p downregulates advanced glycosylation end product receptor expression and improves renal function in diabetic nephropathy mice. Eur Rev Med Pharmacol Sci, 24(9), 5018-5027.

Shen, C. Y., Lu, C. H., Wu, C. H., Li, K. J., Kuo, Y. M., Hsieh, S. C., & Yu, C. L. (2020). The development of Maillard reaction, and Advanced Glycation End Product (AGE)-Receptor for AGE (RAGE) signaling inhibitors as novel therapeutic strategies for patients with age-related diseases. Molecules, 25(23), 5591.

Serveaux-Dancer, M., Jabaudon, M., Creveaux, I., Belville, C., Blondonnet, R., Gross, C., ... & Sapin, V. (2019). Pathological implications of receptor for advanced glycation end-product (AGER) gene polymorphism. Disease markers, 2019.

Yang, Q., Tang, Y., Tang, C., Cong, H., Wang, X., Shen, X., & Ju, S. (2019). Diminished LINC00173 expression induced miR-182-5p accumulation promotes cell proliferation, migration and apoptosis inhibition via AGER/NF-κB pathway in non-small-cell lung cancer. American journal of translational research, 11(7), 4248.

Ghafouri-Fard, S., Noroozi, R., Musavi, M., & Taheri, M. (2019). Association analysis between genomic variants within advanced glycation end product specific receptor (AGER) gene and risk of breast cancer in Iranian women. Heliyon, 5(10), e02542.

Li, P., Tang, Z., Wang, L., & Feng, B. (2017). Glucagon-like peptide-1 analogue liraglutide ameliorates atherogenesis via inhibiting advanced glycation end product-induced receptor for advanced glycosylation end product expression in apolipoprotein-E deficient mice. Molecular medicine reports, 16(3), 3421-3426.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant AGER protein, hFc Tag (CAT#: V2LY-0526-LY6450)

Human Recombinant AGER protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY6451)

Human Recombinant AGER protein, His Tag (CAT#: V2LY-0526-LY6452)

Human Recombinant AGER protein (CAT#: V2LY-0526-LY6453)

Mouse Recombinant AGER protein, His Tag (CAT#: V2LY-0526-LY8821)

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Human Recombinant AGER protein (CAT#: V2LY-0526-LY6453)

Human Recombinant AGER protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY6451)

Human Recombinant AGER protein, His Tag (CAT#: V2LY-0526-LY6452)

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Mouse Recombinant AGER protein, His Tag (CAT#: V2LY-0526-LY8821)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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