Human Recombinant AIF1 protein, His Tag (V2LY-0526-LY4618)

Name: Human Recombinant AIF1 protein, His Tag
SKU: V2LY-0526-LY4618
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant AIF1 protein, His Tag consist of Amino Acid: 1-147 and predicts a molecular mass of 18.5 kDa.

Molecule Mass

18.5 kDa

Sequence

Amino Acid: 1-147

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Allograft Inflammatory Factor 1

Alternative Names

Allograft Inflammatory Factor 1; Ionized Calcium-Binding Adapter Molecule 1; Interferon Gamma Responsive Transcript; Protein G1; AIF-1; IBA1; IRT-1; IRT1; G1;

Function

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Biological Process

Actin crosslink formation Source: ARUK-UCL
Actin filament bundle assembly Source: UniProtKB
Actin filament polymerization Source: UniProtKB
Cellular response to interferon-gamma Source: UniProtKB
Cellular response to oxidative stress Source: ARUK-UCL
Inflammatory response Source: UniProtKB
Microglial cell activation Source: UniProtKB
Negative regulation of smooth muscle cell chemotaxis Source: UniProtKB
Negative regulation of smooth muscle cell proliferation Source: UniProtKB
Parallel actin filament bundle assembly Source: ARUK-UCL
Phagocytosis, engulfment Source: UniProtKB
Positive regulation of cell migration Source: ARUK-UCL
Positive regulation of cell population proliferation Source: ARUK-UCL
Positive regulation of chemokine production Source: ARUK-UCL
Positive regulation of chemotaxis Source: ARUK-UCL
Positive regulation of fibroblast growth factor production Source: ARUK-UCL
Positive regulation of G1/S transition of mitotic cell cycle Source: UniProtKB
Positive regulation of interleukin-6 production Source: ARUK-UCL
Positive regulation of monocyte chemotaxis Source: UniProtKB
Positive regulation of mononuclear cell migration Source: ARUK-UCL
Positive regulation of smooth muscle cell chemotaxis Source: UniProtKB
Positive regulation of smooth muscle cell proliferation Source: UniProtKB
Positive regulation of T cell migration Source: UniProtKB
Positive regulation of T cell proliferation Source: UniProtKB
Rac protein signal transduction Source: UniProtKB
Regulation of gene expression Source: ARUK-UCL
Ruffle assembly Source: UniProtKB

Cellular Location

Ruffle membrane; Cytoplasmic side; Cytoskeleton; Phagocytic cup. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.

PTM

Phosphorylated on serine residues.

da Silva, R. L., Elizondo, D. M., Brandy, N. Z., Haddock, N. L., Boddie, T. A., de Oliveira, L. L., ... & Lipscomb, M. W. (2021). Leishmania donovani infection suppresses Allograft Inflammatory Factor-1 in monocytes and macrophages to inhibit inflammatory responses. Scientific reports, 11(1), 1-10.

Sikora, M., Kopeć, B., Piotrowska, K., & Pawlik, A. (2020). Role of allograft inflammatory factor-1 in pathogenesis of diseases. Immunology letters, 218, 1-4.

Vizioli, J., Verri, T., & Pagliara, P. (2020). Allograft inflammatory factor-1 in metazoans: Focus on invertebrates. Biology, 9(11), 355.

Egaña-Gorroño, L., Chinnasamy, P., Casimiro, I., Almonte, V. M., Parikh, D., Oliveira-Paula, G. H., ... & Sibinga, N. E. (2019). Allograft inflammatory factor-1 supports macrophage survival and efferocytosis and limits necrosis in atherosclerotic plaques. Atherosclerosis, 289, 184-194.

Elizondo, D. M., Brandy, N. Z., da Silva, R. L., Haddock, N. L., Kacsinta, A. D., de Moura, T. R., & Lipscomb, M. W. (2019). Allograft inflammatory factor-1 governs hematopoietic stem cell differentiation into cDC1 and monocyte-derived dendritic cells through IRF8 and RelB in vitro. Frontiers in immunology, 10, 173.

Zhou, Y., Li, X., Yuan, X., & Hao, L. (2019). Role of allograft inflammatory factor‐1 in the regulation of inflammation and oxidative stress in primary peritoneal mesothelial cells. Cell biology international, 43(5), 495-505.

Donovan, K. M., Leidinger, M. R., McQuillen, L. P., Goeken, J. A., Hogan, C. M., Harwani, S. C., ... & Meyerholz, D. K. (2018). Allograft inflammatory factor 1 as an immunohistochemical marker for macrophages in multiple tissues and laboratory animal species. Comparative medicine, 68(5), 341-348.

Ren, J., Lin, Y., Tang, J., Yue, H., & Zhao, Y. (2018). Allograft inflammatory factor-1 mediates macrophage-induced impairment of insulin signaling in adipocytes. Cellular Physiology and Biochemistry, 47(1), 403-413.

Elizondo, D. M., Andargie, T. E., Yang, D., Kacsinta, A. D., & Lipscomb, M. W. (2017). Inhibition of allograft inflammatory factor-1 in dendritic cells restrains CD4+ T cell effector responses and induces CD25+ Foxp3+ T regulatory subsets. Frontiers in immunology, 8, 1502.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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