Human Recombinant AIMP1 protein, His Tag (V2LY-0526-LY3831)

Name: Human Recombinant AIMP1 protein, His Tag
SKU: V2LY-0526-LY3831
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant AIMP1 protein, His Tag consist of Amino Acid: 2-312 and predicts a molecular mass of 35 kDa.

Molecule Mass

35 kDa

Sequence

Amino Acid: 2-312

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS, NaCl, Glycerol, TCEP

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Aminoacyl TRNA Synthetase Complex Interacting Multifunctional Protein 1

Function

Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase (PubMed:10358004). Binds tRNA. Possesses inflammatory cytokine activity (PubMed:11306575). Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation (By similarity). Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels (By similarity). Promotes dermal fibroblast proliferation and wound repair (PubMed:16472771). Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum (By similarity). Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations (PubMed:12237313). Induces maturation of dendritic cells and monocyte cell adhesion (PubMed:11818442). Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7 (PubMed:19362550).

Biological Process

Angiogenesis Source: UniProtKB-KW
Apoptotic process Source: UniProtKB-KW
Cell-cell signaling Source: HGNC-UCL
Defense response to virus Source: Ensembl
Inflammatory response Source: UniProtKB-KW
Leukocyte migration Source: HGNC-UCL
Negative regulation of endothelial cell proliferation Source: HGNC-UCL
Positive regulation of glucagon secretion Source: UniProtKB
tRNA aminoacylation for protein translation Source: Reactome

Cellular Location

Golgi apparatus; Secreted; Nucleus; Endoplasmic reticulum; Cytosol. Enriched in secretory vesicles of pancreatic alpha cells and secreted from the pancreas in response to low glucose levels (By similarity). Secreted in response to hypoxia (PubMed:10850427). Also secreted in response to both apoptotic and necrotic cell death.

Involvement in disease

Leukodystrophy, hypomyelinating, 3 (HLD3): A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system.

PTM

Cleaved by caspase-7 in response to apoptosis to produce EMAP-II.

Lee, D. D., Hochstetler, A., Sah, E., Xu, H., Lowe, C. W., Santiaguel, S., ... & Schwarz, M. A. (2020). Influence of aminoacyl-tRNA synthetase complex-interacting multifunctional protein 1 on epithelial differentiation and organization during lung development. American Journal of Physiology-Lung Cellular and Molecular Physiology, 319(2), L369-L379.

Mun, C. H., Kim, J. O., Ahn, S. S., Yoon, T., Kim, S. J., Ko, E., ... & Park, S. G. (2019). Atializumab, a humanized anti-aminoacyl-tRNA synthetase-interacting multifunctional protein-1 (AIMP1) antibody significantly improves nephritis in (NZB/NZW) F1 mice. Biomaterials, 220, 119408.

Ahn, S. S., Kim, J. O., Yoon, T., Song, J. J., Park, Y. B., Lee, S. W., & Park, S. G. (2019). Serum aminoacyl-tRNA synthetase-interacting multifunctional protein-1 can predict severe antineutrophil cytoplasmic antibody-associated vasculitis: a pilot monocentric study. BioMed research international, 2019.

Accogli, A., Russell, L., Sébire, G., Rivière, J. B., St-Onge, J., Addour-Boudrahem, N., ... & Srour, M. (2019). Pathogenic variants in AIMP1 cause pontocerebellar hypoplasia. neurogenetics, 20(2), 103-108.

Khan, A., Bennett, J., Scantlebury, M. H., Wei, X. C., & Kerr, M. (2019). AIMP1 mutation long-term follow-up, with decreased brain N-acetylaspartic acid and secondary mitochondrial abnormalities. Child neurology open, 6, 2329048X19829520.

Kim, M. S., Song, J. H., Cohen, E. P., Cho, D., & Kim, T. S. (2017). Aminoacyl tRNA synthetase–interacting multifunctional protein 1 activates NK cells via macrophages in vitro and in vivo. The Journal of Immunology, 198(10), 4140-4147.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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