Human Recombinant BID protein, GST Tag (V2LY-0526-LY2351)

Name: Human Recombinant BID protein, GST Tag
SKU: V2LY-0526-LY2351
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant BID protein, GST Tag consist of Amino Acid: Full Length and predicts a molecular mass of 52 kDa.

Molecule Mass

52 kDa

Sequence

Amino Acid: Full Length

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

BH3 Interacting Domain Death Agonist

Function

The major proteolytic product p15 BID allows the release of cytochrome c (By similarity).
Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: GO_Central
Apoptotic mitochondrial changes Source: GO_Central
Establishment of protein localization to membrane Source: BHF-UCL
Extrinsic apoptotic signaling pathway via death domain receptors Source: ProtInc
Hepatocyte apoptotic process Source: Ensembl
Mitochondrial ATP synthesis coupled electron transport Source: Ensembl
Mitochondrial outer membrane permeabilization Source: Ensembl
Negative regulation of apoptotic process Source: Reactome
Negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage Source: Ensembl
Neuron apoptotic process Source: HGNC-UCL
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
Positive regulation of fibroblast apoptotic process Source: Ensembl
Positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
Positive regulation of mitochondrial membrane potential Source: Ensembl
Positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway Source: Reactome
Positive regulation of protein-containing complex assembly Source: UniProtKB
Positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: Reactome
Positive regulation of release of cytochrome c from mitochondria Source: UniProtKB
Protein-containing complex assembly Source: Ensembl
Protein targeting to mitochondrion Source: Ensembl
Regulation of apoptotic process Source: Reactome
Regulation of cell population proliferation Source: Ensembl
Regulation of G1/S transition of mitotic cell cycle Source: Ensembl
Release of cytochrome c from mitochondria Source: HGNC-UCL
Signal transduction in response to DNA damage Source: UniProtKB

Cellular Location

Mitochondrion membrane; Mitochondrion outer membrane; Cytoplasm. When uncleaved, it is predominantly cytoplasmic.
BH3-interacting domain death agonist p15: Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
BH3-interacting domain death agonist p13: Mitochondrion membrane. Associated with the mitochondrial membrane.
Isoform 1: Cytoplasm
Isoform 2: Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively.
Isoform 3: Cytoplasm

PTM

TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.

Maxa, M., Schaeper, U., Dames, S., Vollmar, B., & Kuhla, A. (2019). Liver-specific bid silencing inhibits apap-induced cell death in mice. Apoptosis, 24(11), 934-945.

Wicki, S., Gurzeler, U., Corazza, N., Genitsch, V., Wong, W. W. L., & Kaufmann, T. (2018). Loss of BID delays FASL-induced cell death of mouse neutrophils and aggravates DSS-induced weight loss. International journal of molecular sciences, 19(3), 684.

Tsuno, T., Kanno, S., Ryoichi, I., Tanigawa, N., & Ueba, T. (2018). Abstract B13: New drug repositioning of low-dose pegylated IFN-alpha in combination with BH3 interacting domain death agonist gene and radiation therapy as a cancer treatment regimen.

Zhang, F., Qiu, Q., Song, X., Chen, Y., Wu, J., & Liang, M. (2017). Signal‐Regulated Protein Kinases/Protein Kinase B‐p53‐BH3–Interacting Domain Death Agonist Pathway Regulates Gingipain‐Induced Apoptosis in Osteoblasts. Journal of periodontology, 88(11), e200-e210.

Hu, W. L., Dong, H. Y., Li, Y., Ojcius, D. M., Li, S. J., & Yan, J. (2017). Bid-induced release of AIF/EndoG from mitochondria causes apoptosis of macrophages during infection with Leptospira interrogans. Frontiers in cellular and infection microbiology, 7, 471.

Gao, X., Liu, Y., Xie, Y., Wang, Y., & Qi, S. (2017). Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway. Molecular medicine reports, 16(1), 515-522.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human BID ELISA Kit (CAT#: V2LY-0626-LY2011)

Rat Bid ELISA Kit (CAT#: V2LY-0626-LY210)

Human Recombinant BID protein (CAT#: V2LY-0526-LY2350)

Mouse Recombinant BID protein, GST Tag (CAT#: V2LY-0526-LY7872)

BID Matched Antibody Pair (136) (CAT#: APMAB-136LY)

BID Matched Antibody Pair (137) (CAT#: APMAB-137LY)

Antibody Pairs

BID Matched Antibody Pair (136) (CAT#: APMAB-136LY)

BID Matched Antibody Pair (137) (CAT#: APMAB-137LY)

Related Products

BID Matched Antibody Pair (136) (CAT#: APMAB-136LY)

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Human BID ELISA Kit (CAT#: V2LY-0626-LY2011)

Mouse Anti-BID Recombinant Antibody (CBYY-0565) (CAT#: CBMAB-0568-YY)

Rabbit Anti-Bid Recombinant Antibody (CBYY-0570) (CAT#: CBMAB-0573-YY)

Mouse Recombinant BID protein, GST Tag (CAT#: V2LY-0526-LY7872)

Mouse Anti-BID Recombinant Antibody (CBT213) (CAT#: V2LY-0625-LY3701)

Mouse Anti-BID Recombinant Antibody (CBYY-0564) (CAT#: CBMAB-0567-YY)

Mouse Anti-BID Recombinant Antibody (CBYY-0566) (CAT#: CBMAB-0569-YY)

BID Matched Antibody Pair (137) (CAT#: APMAB-137LY)

Isotype control

For research use only. Not intended for any clinical use.

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