Mouse Recombinant BID protein, GST Tag (V2LY-0526-LY7872)

Name: Mouse Recombinant BID protein, GST Tag
SKU: V2LY-0526-LY7872
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Mouse

Tag

GST Tag

Protein Construction

This product is Mouse Recombinant BID protein, GST Tag consist of Amino Acid: 1-195 and predicts a molecular mass of 50 kDa.

Molecule Mass

50 kDa

Sequence

Amino Acid: 1-195

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Urea

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

BH3 Interacting Domain Death Agonist

Function

The major proteolytic product p15 BID allows the release of cytochrome c (By similarity).
Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: GO_Central
Apoptotic mitochondrial changes Source: GO_Central
Establishment of protein localization to membrane Source: BHF-UCL
Extrinsic apoptotic signaling pathway via death domain receptors Source: ProtInc
Hepatocyte apoptotic process Source: Ensembl
Mitochondrial ATP synthesis coupled electron transport Source: Ensembl
Mitochondrial outer membrane permeabilization Source: Ensembl
Negative regulation of apoptotic process Source: Reactome
Negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage Source: Ensembl
Neuron apoptotic process Source: HGNC-UCL
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
Positive regulation of fibroblast apoptotic process Source: Ensembl
Positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
Positive regulation of mitochondrial membrane potential Source: Ensembl
Positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway Source: Reactome
Positive regulation of protein-containing complex assembly Source: UniProtKB
Positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: Reactome
Positive regulation of release of cytochrome c from mitochondria Source: UniProtKB
Protein-containing complex assembly Source: Ensembl
Protein targeting to mitochondrion Source: Ensembl
Regulation of apoptotic process Source: Reactome
Regulation of cell population proliferation Source: Ensembl
Regulation of G1/S transition of mitotic cell cycle Source: Ensembl
Release of cytochrome c from mitochondria Source: HGNC-UCL
Signal transduction in response to DNA damage Source: UniProtKB

Cellular Location

Mitochondrion membrane; Mitochondrion outer membrane; Cytoplasm. When uncleaved, it is predominantly cytoplasmic.
BH3-interacting domain death agonist p15: Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
BH3-interacting domain death agonist p13: Mitochondrion membrane. Associated with the mitochondrial membrane.
Isoform 1: Cytoplasm
Isoform 2: Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively.
Isoform 3: Cytoplasm

PTM

TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.

Maxa, M., Schaeper, U., Dames, S., Vollmar, B., & Kuhla, A. (2019). Liver-specific bid silencing inhibits apap-induced cell death in mice. Apoptosis, 24(11), 934-945.

Wicki, S., Gurzeler, U., Corazza, N., Genitsch, V., Wong, W. W. L., & Kaufmann, T. (2018). Loss of BID delays FASL-induced cell death of mouse neutrophils and aggravates DSS-induced weight loss. International journal of molecular sciences, 19(3), 684.

Tsuno, T., Kanno, S., Ryoichi, I., Tanigawa, N., & Ueba, T. (2018). Abstract B13: New drug repositioning of low-dose pegylated IFN-alpha in combination with BH3 interacting domain death agonist gene and radiation therapy as a cancer treatment regimen.

Zhang, F., Qiu, Q., Song, X., Chen, Y., Wu, J., & Liang, M. (2017). Signal‐Regulated Protein Kinases/Protein Kinase B‐p53‐BH3–Interacting Domain Death Agonist Pathway Regulates Gingipain‐Induced Apoptosis in Osteoblasts. Journal of periodontology, 88(11), e200-e210.

Hu, W. L., Dong, H. Y., Li, Y., Ojcius, D. M., Li, S. J., & Yan, J. (2017). Bid-induced release of AIF/EndoG from mitochondria causes apoptosis of macrophages during infection with Leptospira interrogans. Frontiers in cellular and infection microbiology, 7, 471.

Gao, X., Liu, Y., Xie, Y., Wang, Y., & Qi, S. (2017). Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway. Molecular medicine reports, 16(1), 515-522.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human BID ELISA Kit (CAT#: V2LY-0626-LY2011)

Rat Bid ELISA Kit (CAT#: V2LY-0626-LY210)

Human Recombinant BID protein (CAT#: V2LY-0526-LY2350)

Human Recombinant BID protein, GST Tag (CAT#: V2LY-0526-LY2351)

BID Matched Antibody Pair (136) (CAT#: APMAB-136LY)

BID Matched Antibody Pair (137) (CAT#: APMAB-137LY)

Antibody Pairs

BID Matched Antibody Pair (136) (CAT#: APMAB-136LY)

BID Matched Antibody Pair (137) (CAT#: APMAB-137LY)

Related Products

Mouse Anti-BID Recombinant Antibody (CBYY-0566) (CAT#: CBMAB-0569-YY)

Mouse Anti-BID Recombinant Antibody (CBT3883) (CAT#: V2LY-0625-LY2631)

Rat Bid ELISA Kit (CAT#: V2LY-0626-LY210)

Mouse Anti-BID Recombinant Antibody (CBYY-0564) (CAT#: CBMAB-0567-YY)

BID Matched Antibody Pair (137) (CAT#: APMAB-137LY)

Human BID ELISA Kit (CAT#: V2LY-0626-LY2011)

Mouse Anti-BID Recombinant Antibody (CBT213) (CAT#: V2LY-0625-LY3701)

Mouse Anti-BID Recombinant Antibody (3F3-1A3) (CAT#: CBMAB-A0762-LY)

Mouse Anti-BID Recombinant Antibody (3C4C2) (CAT#: CBMAB-P0103-YC)

BID Matched Antibody Pair (136) (CAT#: APMAB-136LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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