Human Recombinant BIN1 protein, His Tag (V2LY-0526-LY2357)

Name: Human Recombinant BIN1 protein, His Tag
SKU: V2LY-0526-LY2357
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant BIN1 protein, His Tag consist of Amino Acid: 1-424 and predicts a molecular mass of 49.3 kDa.

Molecule Mass

49.3 kDa

Sequence

Amino Acid: 1-424

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Bridging Integrator 1

Function

Is a key player in the control of plasma membrane curvature, membrane shaping and membrane remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling (PubMed:24755653).
Is a negative regulator of endocytosis (By similarity).
Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production (PubMed:27179792).
In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates (By similarity).
May be involved in the regulation of MYC activity and the control cell proliferation (PubMed:8782822).
Has actin bundling activity and stabilizes actin filaments against depolymerization in vitro (PubMed:28893863).

Biological Process

Cytoskeleton organization Source: ARUK-UCL
Endocytosis Source: ARUK-UCL
Endosome to lysosome transport Source: ARUK-UCL
Lipid tube assembly Source: Alzheimers_University_of_Toronto
Membrane organization Source: Reactome
Negative regulation of amyloid-beta formation Source: ARUK-UCL
Negative regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process Source: ARUK-UCL
Negative regulation of calcium ion transmembrane transport via high voltage-gated calcium channel Source: ARUK-UCL
Negative regulation of potassium ion transmembrane transport Source: ARUK-UCL
Negative regulation of ventricular cardiac muscle cell action potential Source: ARUK-UCL
Nucleus localization Source: Ensembl
Nucleus organization Source: WormBase
Positive regulation of actin filament polymerization Source: ARUK-UCL
Positive regulation of apoptotic process Source: AgBase
Positive regulation of astrocyte differentiation Source: Alzheimers_University_of_Toronto
Regulation of cell cycle arrest Source: Alzheimers_University_of_Toronto
Regulation of endocytosis Source: InterPro
Regulation of heart rate by cardiac conduction Source: ARUK-UCL
Regulation of neuron differentiation Source: Alzheimers_University_of_Toronto
T-tubule organization Source: UniProtKB
Viral process Source: UniProtKB-KW

Cellular Location

Isoform BIN1: Nucleus; T-tubule; Cytoplasm; Endosome
Isoform IIA: Cytoplasm

Involvement in disease

Myopathy, centronuclear, 2 (CNM2): A congenital muscle disorder characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
BIN1 mutations have been found in families segregating autosomal dominant centronuclear myopathy. Patients show adult-onset, mildly progressive muscle weakness affecting selected proximal muscles and all distal muscles of the lower limbs.

PTM

Phosphorylated by protein kinase C.

Hu, H., Tan, L., Bi, Y. L., Xu, W., Tan, L., Shen, X. N., ... & Yu, J. T. (2021). Association between methylation of BIN1 promoter in peripheral blood and preclinical Alzheimer’s disease. Translational psychiatry, 11(1), 1-13.

Glennon, E. B., Lau, D. H., Gabriele, R. M., Taylor, M. F., Troakes, C., Opie-Martin, S., ... & Noble, W. (2020). Bridging integrator 1 protein loss in Alzheimer’s disease promotes synaptic tau accumulation and disrupts tau release. Brain communications, 2(1), fcaa011.

Gao, P., Ye, L., Cheng, H., & Li, H. (2020). The Mechanistic Role of Bridging Integrator 1 (BIN1) in Alzheimer’s Disease. Cellular and Molecular Neurobiology, 1-10.

Wang, J., Liu, T., Wang, M., Lv, W., Wang, Y., Jia, Y., ... & Liu, L. (2020). SRSF1‐dependent alternative splicing attenuates BIN1 expression in non–small cell lung cancer. Journal of cellular biochemistry, 121(2), 946-953.

Zheng, J., Wang, J., Jia, Y., Liu, T., Duan, Y., Liang, X., & Liu, L. (2019). microRNA‐211 promotes proliferation, migration, and invasion ability of oral squamous cell carcinoma cells via targeting the bridging integrator 1 protein. Journal of cellular biochemistry, 120(3), 4644-4653.

Glennon, E. B., Lau, D. H., Gabriele, R. M., Taylor, M. F., Troakes, C., Elliott, C., ... & Noble, W. (2019). Loss of the Alzheimer’s-linked bridging integrator 1 (BIN1) protein affects synaptic structure and disrupts tau localisation and release. bioRxiv, 646406.

Franzmeier, N., Rubinski, A., Neitzel, J., & Ewers, M. (2019). The BIN1 rs744373 SNP is associated with increased tau-PET levels and impaired memory. Nature communications, 10(1), 1-12.

Crotti, A., Sait, H. R., McAvoy, K. M., Estrada, K., Ergun, A., Szak, S., ... & Ransohoff, R. M. (2019). BIN1 favors the spreading of Tau via extracellular vesicles. Scientific reports, 9(1), 1-20.

Pinali, C., Malik, N., Davenport, J. B., Allan, L. J., Murfitt, L., Iqbal, M. M., ... & Kitmitto, A. (2017). Post‐myocardial infarction t‐tubules form enlarged branched structures with dysregulation of junctophilin‐2 and bridging integrator 1 (BIN‐1). Journal of the American Heart Association, 6(5), e004834.

Malki, I., Cantrelle, F. X., Sottejeau, Y., Lippens, G., Lambert, J. C., & Landrieu, I. (2017). Regulation of the interaction between the neuronal BIN 1 isoform 1 and Tau proteins–role of the SH 3 domain. The FEBS journal, 284(19), 3218-3229.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human BIN1 ELISA Kit (CAT#: V2LY-0626-LY3474)

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Isotype control

For research use only. Not intended for any clinical use.

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