Human Recombinant C1QB protein, His Tag (V2LY-0526-LY2454)

Name: Human Recombinant C1QB protein, His Tag
SKU: V2LY-0526-LY2454
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant C1QB protein, His Tag consist of Amino Acid: 1-253 and predicts a molecular mass of 25.2 kDa.

Molecule Mass

25.2 kDa

Sequence

Amino Acid: 1-253

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

complement C1q B chain

Function

C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca2+-dependent C1r2C1s2 proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

Biological Process

Complement activation Source: Reactome
Complement activation, classical pathway Source: Reactome
Innate immune response Source: UniProtKB-KW
Regulation of complement activation Source: Reactome
Synapse pruning Source: ARUK-UCL

Cellular Location

Secreted

Involvement in disease

Complement component C1q deficiency (C1QD): A disorder caused by impaired activation of the complement classical pathway. It generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.

PTM

Hydroxylated on lysine and proline residues. Hydroxylated lysine residues can be glycosylated. Human C1Q contains up to 68.3 hydroxylysine-galactosylglucose residues and up to 2.5 hydroxylysine-galactose per molecule. Total percentage hydroxylysine residues glycosylated is 86.4%.

Chen, L. H., Liu, J. F., Lu, Y., He, X. Y., Zhang, C., & Zhou, H. H. (2021). Complement C1q (C1qA, C1qB, and C1qC) May Be a Potential Prognostic Factor and an Index of Tumor Microenvironment Remodeling in Osteosarcoma. Frontiers in oncology, 11, 1643.

Mamoor, S. (2021). Chain A, B, and C of the complement component C1q are differentially expressed in human epithelial ovarian cancer.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

C1QB Matched Antibody Pair (155) (CAT#: APMAB-155LY)

Antibody Pairs

C1QB Matched Antibody Pair (155) (CAT#: APMAB-155LY)

Related Products

C1QB Matched Antibody Pair (155) (CAT#: APMAB-155LY)

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Mouse Anti-C1QB Recombinant Antibody (1236) (CAT#: CBMAB-1236-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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