Human Recombinant CALM2 protein, His Tag (V2LY-0526-LY2512)

Name: Human Recombinant CALM2 protein, His Tag
SKU: V2LY-0526-LY2512
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CALM2 protein, His Tag consist of Amino Acid: 1-149 and predicts a molecular mass of 18.7 kDa.

Molecule Mass

18.7 kDa

Sequence

Amino Acid: 1-149

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile HEPES, NaCl

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

calmodulin 2 (phosphorylase kinase, delta)

Function

Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (PubMed:16760425).
Mediates calcium-dependent inactivation of CACNA1C (PubMed:26969752).
Positively regulates calcium-activated potassium channel activity of KCNN2 (PubMed:27165696).

Biological Process

Detection of calcium ion Source: BHF-UCL
G protein-coupled receptor signaling pathway Source: UniProtKB
Negative regulation of peptidyl-threonine phosphorylation Source: BHF-UCL
Negative regulation of ryanodine-sensitive calcium-release channel activity Source: UniProtKB
Positive regulation of cyclic-nucleotide phosphodiesterase activity Source: BHF-UCL
Positive regulation of peptidyl-threonine phosphorylation Source: BHF-UCL
Positive regulation of phosphoprotein phosphatase activity Source: BHF-UCL
Positive regulation of protein autophosphorylation Source: BHF-UCL
Positive regulation of protein dephosphorylation Source: BHF-UCL
Positive regulation of protein serine/threonine kinase activity Source: BHF-UCL
Positive regulation of ryanodine-sensitive calcium-release channel activity Source: BHF-UCL
Regulation of cardiac muscle contraction Source: BHF-UCL
Regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion Source: BHF-UCL
Regulation of cell communication by electrical coupling involved in cardiac conduction Source: BHF-UCL
Regulation of cytokinesis Source: UniProtKB
Regulation of heart rate Source: BHF-UCL
Regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: BHF-UCL
Regulation of ryanodine-sensitive calcium-release channel activity Source: GO_Central
Response to calcium ion Source: BHF-UCL
Substantia nigra development Source: UniProtKB

Cellular Location

Spindle; Spindle pole; Centrosome. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.

Involvement in disease

Long QT syndrome 15 (LQT15): A form of long QT syndrome, a heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress, and can present with a sentinel event of sudden cardiac death in infancy.

PTM

Ubiquitination results in a strongly decreased activity.
Phosphorylation results in a decreased activity.

Ma, B., Zhao, M., & Guo, Z. (2021). Circular RNA circ_0010729 Knockdown Attenuates Oxygen–Glucose Deprivation-Induced Human Cardiac Myocytes Injury by miR-338-3p/CALM2 Axis. Journal of Cardiovascular Pharmacology, 77(5), 594-602.

Zhang, Y., Hou, Y. M., Gao, F., Xiao, J. W., Li, C. C., & Tang, Y. (2019). lncRNA GAS5 regulates myocardial infarction by targeting the miR‐525‐5p/CALM2 axis. Journal of cellular biochemistry, 120(11), 18678-18688.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

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Mouse Anti-CALM2 Recombinant Antibody (J4D8) (CAT#: CBMAB-C1433-LY)

Mouse Anti-CALM2 Recombinant Antibody (2D1) (CAT#: CBMAB-C3348-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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