Human Recombinant CALML3 protein, His Tag (V2LY-0526-LY2513)

Name: Human Recombinant CALML3 protein, His Tag
SKU: V2LY-0526-LY2513
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CALML3 protein, His Tag consist of Amino Acid: 1-149 and predicts a molecular mass of 18.7 kDa.

Molecule Mass

18.7 kDa

Sequence

Amino Acid: 1-149

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile HEPES, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Calmodulin Like 3

Function

May function as a specific light chain of unconventional myosin-10 (MYO10), also enhances MYO10 translation, possibly by acting as a chaperone for the emerging MYO10 heavy chain protein. May compete with calmodulin by binding, with different affinities, to cellular substrates.

Cellular Location

Extracellular exosome

Chen, G., Yu, C., Tang, Z., Liu, S., An, F., Zhu, J., ... & Zhang, S. (2019). Metformin suppresses gastric cancer progression through calmodulin‑like protein 3 secreted from tumor‑associated fibroblasts. Oncology reports, 41(1), 405-414.

Jia, Y., Qin, Q., Fang, C. P., Shen, W., Sun, T. T., Huang, Y. L., ... & Deng, A. M. (2018). UVB induces apoptosis via downregulation of CALML3-dependent JNK1/2 and ERK1/2 pathways in cataract. International journal of molecular medicine, 41(5), 3041-3050.

Qin, S., Ingle, J. N., Liu, M., Yu, J., Wickerham, D. L., Kubo, M., ... & Wang, L. (2017). Calmodulin-like protein 3 is an estrogen receptor alpha coregulator for gene expression and drug response in a SNP, estrogen, and SERM-dependent fashion. Breast Cancer Research, 19(1), 1-14.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

CALML3 Matched Antibody Pair (170) (CAT#: APMAB-170LY)

CALML3 Matched Antibody Pair (171) (CAT#: APMAB-171LY)

Antibody Pairs

CALML3 Matched Antibody Pair (170) (CAT#: APMAB-170LY)

CALML3 Matched Antibody Pair (171) (CAT#: APMAB-171LY)

Related Products

Mouse Anti-CALML3 Recombinant Antibody (2A11) (CAT#: CBMAB-C4578-LY)

Mouse Anti-CALML3 Recombinant Antibody (03) (CAT#: CBMAB-C1696-LY)

Rabbit Anti-CALML3 Recombinant Antibody (004) (CAT#: CBMAB-C1505-LY)

CALML3 Matched Antibody Pair (171) (CAT#: APMAB-171LY)

CALML3 Matched Antibody Pair (170) (CAT#: APMAB-170LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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