Human Recombinant CALU protein, His Tag (V2LY-0526-LY2524)

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Basic Information

Expressed Host
HEK293 Cells
Protein Species
Human
Tag
His Tag
Protein Construction
This product is Human Recombinant CALU protein, His Tag consist of Amino Acid: 1-311 and predicts a molecular mass of 35.9 kDa.
Molecule Mass
35.9 kDa
Sequence
Amino Acid: 1-311
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>90% as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile PBS
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
Calumenin
Function
Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity (By similarity).
Biological Process
Cellular protein metabolic process Source: Reactome
Post-translational protein modification Source: Reactome
Cellular Location
Golgi apparatus; Secreted; Endoplasmic reticulum membrane; Melanosome; Sarcoplasmic reticulum lumen. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Du, Y., Miao, W., Jiang, X., Cao, J., Wang, B., Wang, Y., ... & Liu, H. (2021). The Epithelial to Mesenchymal Transition Related Gene Calumenin Is an Adverse Prognostic Factor of Bladder Cancer Correlated With Tumor Microenvironment Remodeling, Gene Mutation, and Ferroptosis. Frontiers in Oncology, 11, 1771.

Yang, Y., Wang, J., Xu, S., Shi, F., & Shan, A. (2021). Calumenin contributes to epithelial-mesenchymal transition and predicts poor survival in glioma. Translational Neuroscience, 12(1), 67-75.

Nasri Nasrabadi, P., Nayeri, Z., Gharib, E., Salmanipour, R., Masoomi, F., Mahjoubi, F., & Zomorodipour, A. (2020). Establishment of a CALU, AURKA, and MCM2 gene panel for discrimination of metastasis from primary colon and lung cancers. Plos one, 15(5), e0233717.

Philippe, R., & Mignen, O. (2019). Measuring calumenin impact on ER-calcium depletion using transient calumenin overexpression and silencing. In Calcium-Binding proteins of the EF-hand superfamily (pp. 539-550). Humana Press, New York, NY.

Narayanasamy, S., & Aradhyam, G. K. (2018). The Differential Response to Ca2+ from Vertebrate and Invertebrate Calumenin Is Governed by a Single Amino Acid Residue. Biochemistry, 57(5), 722-731.

Wang, Y., Sun, Y., Guo, X., Fu, Y., Long, J., Wei, C. X., & Zhao, M. (2018). Creatine phosphate disodium salt protects against dox-induced cardiotoxicity by increasing calumenin. Medical molecular morphology, 51(2), 96-101.

Philippe, R., Antigny, F., Buscaglia, P., Norez, C., Huguet, F., Castelbou, C., ... & Mignen, O. (2017). Calumenin contributes to ER-Ca2+ homeostasis in bronchial epithelial cells expressing WT and F508del mutated CFTR and to F508del-CFTR retention. Cell calcium, 62, 47-59.

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For research use only. Not intended for any clinical use.

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