Human Recombinant CAT protein, His Tag (V2LY-0526-LY2601)

Name: Human Recombinant CAT protein, His Tag
SKU: V2LY-0526-LY2601
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CAT protein, His Tag consist of Amino Acid: 2-527 and predicts a molecular mass of 61.9 kDa.

Molecule Mass

61.9 kDa

Sequence

Amino Acid: 2-527

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>80% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Catalase

Function

Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.

Biological Process

Aerobic respiration Source: Ensembl
Aging Source: Ensembl
Cellular response to growth factor stimulus Source: Ensembl
Cellular response to oxidative stress Source: Reactome
Cholesterol metabolic process Source: Ensembl
Hemoglobin metabolic process Source: Ensembl
Hydrogen peroxide catabolic process Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of NF-kappaB transcription factor activity Source: Ensembl
Neutrophil degranulation Source: Reactome
Osteoblast differentiation Source: UniProtKB
Positive regulation of cell division Source: UniProtKB-KW
Positive regulation of NF-kappaB transcription factor activity Source: Ensembl
Positive regulation of phosphatidylinositol 3-kinase signaling Source: Ensembl
Protein localization Source: Reactome
Response to activity Source: Ensembl
Response to cadmium ion Source: Ensembl
Response to drug Source: Ensembl
Response to estradiol Source: Ensembl
Response to ethanol Source: Ensembl
Response to fatty acid Source: Ensembl
Response to hydrogen peroxide Source: GO_Central
Response to hyperoxia Source: Ensembl
Response to hypoxia Source: Ensembl
Response to inactivity Source: Ensembl
Response to insulin Source: Ensembl
Response to L-ascorbic acid Source: Ensembl
Response to lead ion Source: Ensembl
Response to light intensity Source: Ensembl
Response to ozone Source: Ensembl
Response to phenylpropanoid Source: Ensembl
Response to reactive oxygen species Source: UniProtKB
Response to vitamin A Source: Ensembl
Response to vitamin E Source: Ensembl
Triglyceride metabolic process Source: Ensembl
Ureteric bud development Source: Ensembl
UV protection Source: UniProtKB

Cellular Location

Peroxisome

Involvement in disease

Acatalasemia (ACATLAS): A metabolic disorder characterized by a total or near total loss of catalase activity in red cells. It is often associated with ulcerating oral lesions. Acatalasemia is inherited as an autosomal recessive trait.

PTM

The N-terminus is blocked.

Shi, C., Li, M., Zhang, Z., Yao, Q., Shao, K., Xu, F., ... & Peng, X. (2020). Catalase-based liposomal for reversing immunosuppressive tumor microenvironment and enhanced cancer chemo-photodynamic therapy. Biomaterials, 233, 119755.

Jing, M., Han, G., Wan, J., Zhang, S., Yang, J., Zong, W., ... & Liu, R. (2020). Catalase and superoxide dismutase response and the underlying molecular mechanism for naphthalene. Science of The Total Environment, 736, 139567.

Li, G., Yuan, S., Deng, D., Ou, T., Li, Y., Sun, R., ... & Wu, S. (2019). Fluorinated polyethylenimine to enable transmucosal delivery of photosensitizer‐conjugated catalase for photodynamic therapy of orthotopic bladder tumors postintravesical instillation. Advanced Functional Materials, 29(40), 1901932.

Phua, S. Z. F., Yang, G., Lim, W. Q., Verma, A., Chen, H., Thanabalu, T., & Zhao, Y. (2019). Catalase-integrated hyaluronic acid as nanocarriers for enhanced photodynamic therapy in solid tumor. ACS nano, 13(4), 4742-4751.

Wang, W., Cheng, Y., Chen, D., Liu, D., Hu, M., Dong, J., ... & Shen, F. (2019). The catalase gene family in cotton: genome-wide characterization and bioinformatics analysis. Cells, 8(2), 86.

Tuzet, A., Rahantaniaina, M. S., & Noctor, G. (2019). Analyzing the function of catalase and the ascorbate–glutathione pathway in H2O2 processing: insights from an experimentally constrained kinetic model. Antioxidants & redox signaling, 30(9), 1238-1268.

Zhang, M., Yang, N., Liu, Y., & Tang, J. (2019). Synthesis of catalase-inorganic hybrid nanoflowers via sonication for colorimetric detection of hydrogen peroxide. Enzyme and microbial technology, 128, 22-25.

Xu, M., Cui, Z., Zhao, L., Hu, S., Zong, W., & Liu, R. (2018). Characterizing the binding interactions of PFOA and PFOS with catalase at the molecular level. Chemosphere, 203, 360-367.

Ighodaro, O. M., & Akinloye, O. A. (2018). First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria journal of medicine, 54(4), 287-293.

Chen, L., Zhang, J., Zhu, Y., & Zhang, Y. (2018). Interaction of chromium (III) or chromium (VI) with catalase and its effect on the structure and function of catalase: an in vitro study. Food chemistry, 244, 378-385.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

CAT Matched Antibody Pair (186) (CAT#: APMAB-186LY)

Antibody Pairs

CAT Matched Antibody Pair (186) (CAT#: APMAB-186LY)

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CAT Matched Antibody Pair (186) (CAT#: APMAB-186LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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