Human Recombinant CCNA1 protein, His Tag (V2LY-0526-LY3449)

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Basic Information

Expressed Host
Baculovirus-Insect Cells
Protein Species
Human
Tag
His Tag
Protein Construction
This product is Human Recombinant CCNA1 protein, His Tag consist of Amino Acid: 1-465 and predicts a molecular mass of 54.6 kDa.
Molecule Mass
54.6 kDa
Sequence
Amino Acid: 1-465
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
≥90% as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile PBS
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
Cyclin A1
Function
May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.
Biological Process
Cell division Source: UniProtKB-KW
Male meiosis I Source: ProtInc
Mitotic cell cycle phase transition Source: GO_Central
Protein deubiquitination Source: Reactome
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
Regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Reactome
Spermatogenesis Source: ProtInc
Cellular Location
Nucleus
PTM
Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.

Bourova-Flin, E., Derakhshan, S., Goudarzi, A., Wang, T., Vitte, A. L., Chuffart, F., ... & Aminishakib, P. (2021). The combined detection of Amphiregulin, Cyclin A1 and DDX20/Gemin3 expression predicts aggressive forms of oral squamous cell carcinoma. British Journal of Cancer, 125(8), 1122-1134.

Tsao, C. H., Hsieh, W. C., Yang, R. Y., Lo, Y. H., Tu, T. J., Ke, L. Y., ... & Liu, F. T. (2021). Galectin-12 modulates sebocyte proliferation and cell cycle progression by regulating cyclin A1 and CDK2. Glycobiology.

Ge, M., Xu, Q., Kang, T., Li, D., Wang, R., Chen, Z., ... & Liu, H. (2021). Deubiquitinating enzyme inhibitor alleviates cyclin A1‐mediated proteasome inhibitor tolerance in mixed‐lineage leukemia. Cancer science, 112(6), 2287-2298.

Leung, W. K., Workineh, A., Mukhi, S., Tzannou, I., Brenner, D., Watanabe, N., ... & Lulla, P. (2020). Evaluation of cyclin A1–specific T cells as a potential treatment for acute myeloid leukemia. Blood advances, 4(2), 387-397.

Radonova, L., Pauerova, T., Jansova, D., Danadova, J., Skultety, M., Kubelka, M., & Anger, M. (2020). Cyclin A1 in Oocytes Prevents Chromosome Segregation And Anaphase Entry. Scientific reports, 10(1), 1-12.

He, X., Li, S., Shi, W., Lin, Q., Ma, J., Liu, Y., ... & Cao, X. (2019). Cyclin A1 is associated with poor prognosis in oesophageal squamous cell carcinoma. Oncology letters, 18(1), 706-712.

Pandey, S., Moazam, M., Ghimirey, N., Kuerbitz, S. J., & Fraizer, G. C. (2019). WT1 regulates cyclin A1 expression in K562 cells. Oncology reports, 42(5), 2016-2028.

Cao, J., Dong, J., Wang, Y., & Chen, Y. (2019). The expressions of DNA methyltransferase 1 (DNMT1) and cyclin A1 (CCNA1) in cervical carcinogenesis. International journal of clinical and experimental pathology, 12(1), 40.

Panigrahi, S. K., Manterola, M., & Wolgemuth, D. J. (2017). Meiotic failure in cyclin A1-deficient mouse spermatocytes triggers apoptosis through intrinsic and extrinsic signaling pathways and 14-3-3 proteins. PLoS One, 12(3), e0173926.

Lulla, P., Tzannou, I., Brenner, D., Heslop, H. E., Vera, J. F., & Leen, A. M. (2017). Evaluating Cyclin A1 As an Immunotherapeutic Target in Acute Myeloid Leukemia (AML). Blood, 130, 1907.

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For research use only. Not intended for any clinical use.

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