Human Recombinant CD164 protein, His Tag (V2LY-0526-LY2680)

Name: Human Recombinant CD164 protein, His Tag
SKU: V2LY-0526-LY2680
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CD164 protein, His Tag consist of Amino Acid: 1-162 and predicts a molecular mass of 15.9 kDa.

Molecule Mass

15.9 kDa

Verified

HPLC

Sequence

Amino Acid: 1-162

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD164 molecule, sialomucin

Function

Sialomucin that may play a key role in hematopoiesis by facilitating the adhesion of CD34+ cells to the stroma and by negatively regulating CD34+CD38(lo/-) cell proliferation. Modulates the migration of umbilical cord blood CD133+ cells and this is mediated through the CXCL12/CXCR4 axis. May play an important role in prostate cancer metastasis and the infiltration of bone marrow by cancer cells. Promotes myogenesis by enhancing CXCR4-dependent cell motility. Positively regulates myoblast migration and promotes myoblast fusion into myotubes (By similarity).

Biological Process

Cell adhesion Source: MGI
Hemopoiesis Source: UniProtKB
Heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: MGI
Immune response Source: ProtInc
Multicellular organism development Source: ProtInc
Muscle organ development Source: UniProtKB-KW
Negative regulation of cell adhesion Source: UniProtKB
Negative regulation of cell population proliferation Source: ProtInc
Signal transduction Source: ProtInc

Cellular Location

Lysosome membrane; Endosome membrane; Cell membrane
Isoform 2: Secreted

Involvement in disease

Deafness, autosomal dominant, 66 (DFNA66): A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

Topology

Extracellular: 24-162
Helical: 163-183
Cytoplasmic: 184-197

PTM

Highly N- and O-glycosylated; contains sialic acid.
The motif Ser-Gly may serve as the site of attachment of a glycosaminoglycan side chain.

Watt, S. M., Bühring, H. J., Simmons, P. J., & Zannettino, A. W. (2021). The stem cell revolution: on the role of CD164 as a human stem cell marker. NPJ Regenerative Medicine, 6(1), 1-6.

Mancarella, C., Caldoni, G., Ribolsi, I., Parra, A., Manara, M. C., Mercurio, A. M., ... & Scotlandi, K. (2020). Insulin-Like Growth Factor 2 mRNA-Binding Protein 3 Modulates Aggressiveness of Ewing Sarcoma by Regulating the CD164-CXCR4 Axis. Frontiers in Oncology, 10, 994.

Zhang, L., Wang, Y., & Su, H. (2020). Long non-coding RNA LINC00152/miR-613/CD164 axis regulates cell proliferation, apoptosis, migration and invasion in glioma via PI3K/AKT pathway. Neoplasma, 67(4), 762-772.

Wang, C. C., Hueng, D. Y., Huang, A. F., Chen, W. L., Huang, S. M., & Chan, J. Y. H. (2019). CD164 regulates proliferation, progression, and invasion of human glioblastoma cells. Oncotarget, 10(21), 2041.

Mancarella, C., Manara, M. C., Mercurio, A. M., & Scotlandi, K. (2019). Insulin-like growth factor 2 (IGF-2) mRNA binding protein 3-mediated regulation of CD164-CXCR4 axis impacts aggressiveness of Ewing sarcoma.

Tu, M., & Zheng, W. (2018). CD164 induced the apoptosis of U87 cell by down-regulating PTEN/PI3K/AKT signal pathway in vitro.

Chen, W. L., Huang, A. F., Huang, S. M., Ho, C. L., Chang, Y. L., & Chan, J. Y. H. (2017). CD164 promotes lung tumor-initiating cells with stem cell activity and determines tumor growth and drug resistance via Akt/mTOR signaling. Oncotarget, 8(33), 54115.

Tu, M., Cai, L., Zheng, W., Su, Z., Chen, Y., & Qi, S. (2017). CD164 regulates proliferation and apoptosis by targeting PTEN in human glioma. Molecular medicine reports, 15(4), 1713-1721.

Benoit, B. M., Jariwala, N., O’Connor, G., Oetjen, L. K., Whelan, T. M., Werth, A., ... & Wysocka, M. (2017). CD164 identifies CD4+ T cells highly expressing genes associated with malignancy in Sézary syndrome: the Sézary signature genes, FCRL3, Tox, and miR-214. Archives of dermatological research, 309(1), 11-19.

Chen, J. H., Chen, W. L., Chan, J. Y. H., Chen, Y. W., Peng, Y. J., Cheng, M. F., & Lin, C. S. (2017). Overexpression of CD164 in oral cavity squamous cell carcinoma predicts a favourable prognosis. Oncology letters, 14(5), 6103-6108.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant CD164 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY1404)

Rat Recombinant CD164 protein, hFc Tag (CAT#: V2LY-0526-LY9211)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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