Human Recombinant CD177 protein, His Tag (V2LY-0526-LY2700)

Name: Human Recombinant CD177 protein, His Tag
SKU: V2LY-0526-LY2700
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CD177 protein, His Tag consist of Amino Acid: 1-407 and predicts a molecular mass of 42.7 kDa.

Molecule Mass

42.7 kDa

Verified

HPLC

Sequence

Amino Acid: 1-407

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD177 Molecule

Function

In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed:21193407).
In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed:28807980).
Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed:17580308).
However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed:23461681).
Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed:17244676, PubMed:18462208).
By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed:23202369).

Biological Process

Blood coagulation Source: Reactome
Cell adhesion Source: UniProtKB
Cell-cell adhesion via plasma-membrane adhesion molecules Source: UniProtKB
Cell-cell junction maintenance Source: UniProtKB
Innate immune response Source: UniProtKB-KW
Leukocyte cell-cell adhesion Source: UniProtKB
Leukocyte migration Source: Reactome
Neutrophil degranulation Source: Reactome
Neutrophil extravasation Source: UniProtKB
Neutrophil migration Source: UniProtKB
Positive regulation of neutrophil degranulation Source: UniProtKB
Positive regulation of superoxide anion generation Source: UniProtKB
Protein localization to cell surface Source: UniProtKB
Regulation of endocytosis Source: UniProtKB
Regulation of integrin-mediated signaling pathway Source: UniProtKB

Cellular Location

Cell membrane; Secreted; Membrane raft; Cytoplasmic granule membrane; Lamellipodium. Cell surface expression on neutrophils is increased upon TNF-alpha, fMLP or CXCL8/IL8-mediated stimulation (PubMed:17244676, PubMed:17580308). In neutrophils, stored predominantly in secondary and tertiary granules (PubMed:18462208). Can also be shedded from the cell membrane (PubMed:12239154, PubMed:18462208). Localizes to lamellar protrusions in spreading neutrophils (PubMed:28807980).

PTM

N-glycosylated.
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).

Browne, T., Wroe, E., Keen, L., & Poles, A. (2021). Human neutrophil antigen 2 sequence‐based typing: Joining the hunt for the CD177 answer. Vox Sanguinis.

Saha, R., Pradhan, S. S., Das, P., Mishra, P., Singh, R., Sivaramakrishnan, V., & Acharya, P. (2021). Inflammatory signature in acute-on-chronic liver failure includes increased expression of granulocyte genes ELANE, MPO and CD177. Scientific reports, 11(1), 1-16.

Dahlstrand Rudin, A., Amirbeagi, F., Davidsson, L., Khamzeh, A., Thorbert Mros, S., Thulin, P., ... & Bylund, J. (2021). The neutrophil subset defined by CD177 expression is preferentially recruited to gingival crevicular fluid in periodontitis. Journal of Leukocyte Biology, 109(2), 349-362.

Lévy, Y., Wiedemann, A., Hejblum, B. P., Durand, M., Lefebvre, C., Surénaud, M., ... & French COVID cohort study group. (2021). CD177, a specific marker of neutrophil activation, is associated with coronavirus disease 2019 severity and death. Iscience, 24(7), 102711.

Kim, M. C., Borcherding, N., Ahmed, K. K., Voigt, A. P., Vishwakarma, A., Kolb, R., ... & Zhang, W. (2021). CD177 modulates the function and homeostasis of tumor-infiltrating regulatory T cells. Nature communications, 12(1), 1-13.

Huang, Y. (2019). 4311 Decreased CD177 expression and its association with intravenous immunoglobulin resistance in Kawasaki disease. European Heart Journal, 40(Supplement_1), ehz745-0156.

Deng, H., Hu, N., Wang, C., Chen, M., & Zhao, M. H. (2018). Interaction between CD177 and platelet endothelial cell adhesion molecule-1 downregulates membrane-bound proteinase-3 (PR3) expression on neutrophils and attenuates neutrophil activation induced by PR3-ANCA. Arthritis research & therapy, 20(1), 1-10.

Bai, M., Grieshaber-Bouyer, R., Wang, J., Schmider, A. B., Wilson, Z. S., Zeng, L., ... & Nigrovic, P. A. (2017). CD177 modulates human neutrophil migration through activation-mediated integrin and chemoreceptor regulation. Blood, The Journal of the American Society of Hematology, 130(19), 2092-2100.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD177 protein, hFc Tag (CAT#: V2LY-0526-LY2699)

Human CD177 ELISA Kit (CAT#: V2LY-0626-LY4381)

CD177 Matched Antibody Pair (206) (CAT#: APMAB-206LY)

Antibody Pairs

CD177 Matched Antibody Pair (206) (CAT#: APMAB-206LY)

Related Products

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CD177 Matched Antibody Pair (206) (CAT#: APMAB-206LY)

Mouse Anti-CD177 Recombinant Antibody (CBYY-C0298) (CAT#: CBMAB-C1002-YY)

Human Anti-CD177 Recombinant Antibody (CBYY-C3178) (CAT#: CBMAB-C4621-YY)

Rabbit Anti-CD177 Recombinant Antibody (021) (CAT#: CBMAB-C1675-LY)

Mouse Anti-CD177 Recombinant Antibody (CBT2208) (CAT#: V2LY-0625-LY1156)

Mouse Anti-CD177 Recombinant Antibody (MEM-166) (CAT#: CBMAB-C2200-CQ)

Mouse Anti-CD177 Recombinant Antibody (CBYY-C0159) (CAT#: CBMAB-C0507-YY)

Human CD177 ELISA Kit (CAT#: V2LY-0626-LY4381)

Isotype control

For research use only. Not intended for any clinical use.

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