Human Recombinant CD19 protein, ECD, AF647 Conjugated, His Tag (V2LY-0526-LY2712)

Name: Human Recombinant CD19 protein, ECD, AF647 Conjugated, His Tag
SKU: V2LY-0526-LY2712
Rating: 5 (1 reviews)

See all CD19 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Stable Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CD19 protein, ECD, AF647 Conjugated, His Tag consist of Amino Acid: 1-291 and predicts a molecular mass of 31.6 kDa.

Molecule Mass

31.6 kDa

Protein Domain

ECD

Conjugates

AF647

Sequence

Amino Acid: 1-291

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Preservative

None

Storage

Twelve months from date of receipt at -20°C to -70°C in lyophilized form and 6 months at -70°C under sterile conditions after reconstitution. Protect from prolonged exposure to light and avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD19 Molecule

Alternative Names

CD19 Molecule; B-Lymphocyte Surface Antigen B4; T-Cell Surface Antigen Leu-12; Differentiation Antigen CD19; CD19 Antigen; B-Lymphocyte Antigen CD19; CVID3; B4;

Function

Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:2463100, PubMed:1373518, PubMed:16672701).
Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2+ stores (PubMed:9382888, PubMed:9317126, PubMed:12387743, PubMed:16672701).
Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126).
Required for normal differentiation of B-1 cells (By similarity).
Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:2463100, PubMed:1373518).
Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:9317126, PubMed:12387743, PubMed:16672701).

Biological Process

Antigen receptor-mediated signaling pathway Source: UniProtKB
B-1 B cell differentiation Source: Ensembl
B cell proliferation involved in immune response Source: UniProtKB
B cell receptor signaling pathway Source: UniProtKB
Immunoglobulin mediated immune response Source: UniProtKB
Positive regulation of phosphatidylinositol 3-kinase activity Source: UniProtKB
Positive regulation of protein kinase B signaling Source: Reactome
Positive regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
Regulation of B cell activation Source: UniProtKB
Regulation of B cell receptor signaling pathway Source: UniProtKB
Regulation of complement activation Source: Reactome
Regulation of immune response Source: Reactome

Cellular Location

Cell membrane; Membrane raft

Involvement in disease

Immunodeficiency, common variable, 3 (CVID3): A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.

Topology

Extracellular: 20-291
Helical: 292-313
Cytoplasmic: 314-556

PTM

Phosphorylated on tyrosine following B-cell activation (PubMed:7684160, PubMed:7687539, PubMed:10706702, PubMed:12387743). Phosphorylated on tyrosine residues by LYN (PubMed:7687428). Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels (By similarity).

Spiegel, J. Y., Patel, S., Muffly, L., Hossain, N. M., Oak, J., Baird, J. H., ... & Miklos, D. B. (2021). CAR T cells with dual targeting of CD19 and CD22 in adult patients with recurrent or refractory B cell malignancies: a phase 1 trial. Nature Medicine, 27(8), 1419-1431.

Nerreter, T., Letschert, S., Götz, R., Doose, S., Danhof, S., Einsele, H., ... & Hudecek, M. (2019). Super-resolution microscopy reveals ultra-low CD19 expression on myeloma cells that triggers elimination by CD19 CAR-T. Nature communications, 10(1), 1-11.

Ying, Z., Huang, X. F., Xiang, X., Liu, Y., Kang, X., Song, Y., ... & Chen, S. Y. (2019). A safe and potent anti-CD19 CAR T cell therapy. Nature medicine, 25(6), 947-953.

Hatterer, E., Barba, L., Noraz, N., Daubeuf, B., Aubry-Lachainaye, J. P., von der Weid, B., ... & Buatois, V. (2019, February). Co-engaging CD47 and CD19 with a bispecific antibody abrogates B-cell receptor/CD19 association leading to impaired B-cell proliferation. In MAbs (Vol. 11, No. 2, pp. 322-334). Taylor & Francis.

Salter, A. I., Pont, M. J., & Riddell, S. R. (2018). Chimeric antigen receptor–modified T cells: CD19 and the road beyond. Blood, The Journal of the American Society of Hematology, 131(24), 2621-2629.

Bagashev, A., Sotillo, E., Tang, C. H. A., Black, K. L., Perazzelli, J., Seeholzer, S. H., ... & Thomas-Tikhonenko, A. (2018). CD19 alterations emerging after CD19-directed immunotherapy cause retention of the misfolded protein in the endoplasmic reticulum. Molecular and cellular biology, 38(21), e00383-18.

Turtle, C. J., Hay, K. A., Hanafi, L. A., Li, D., Cherian, S., Chen, X., ... & Maloney, D. G. (2017). Durable molecular remissions in chronic lymphocytic leukemia treated with CD19-specific chimeric antigen receptor–modified T cells after failure of ibrutinib. Journal of Clinical Oncology, 35(26), 3010.

Alabanza, L., Pegues, M., Geldres, C., Shi, V., Wiltzius, J. J., Sievers, S. A., ... & Kochenderfer, J. N. (2017). Function of novel anti-CD19 chimeric antigen receptors with human variable regions is affected by hinge and transmembrane domains. Molecular Therapy, 25(11), 2452-2465.

Li, X., Ding, Y., Zi, M., Sun, L., Zhang, W., Chen, S., & Xu, Y. (2017). CD19, from bench to bedside. Immunology letters, 183, 86-95.

Liu, L., Lam, C. Y. K., Long, V., Widjaja, L., Yang, Y., Li, H., ... & Bonvini, E. (2017). MGD011, A CD19 x CD3 dual-affinity retargeting bi-specific molecule incorporating extended circulating half-life for the treatment of B-cell malignancies. Clinical Cancer Research, 23(6), 1506-1518.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant CD19 protein, ECD, AF647 Conjugated, His Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

CD19 Matched Antibody Pair (207) (CAT#: APMAB-207LY)

CD19 Matched Antibody Pair (208) (CAT#: APMAB-208LY)

Cynomolgus, Rhesus Recombinant CD19 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY24)

Cynomolgus, Rhesus Recombinant CD19 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY1686)

Cynomolgus, Rhesus Recombinant CD19 protein, His Tag (CAT#: V2LY-0526-LY1687)

Human Recombinant CD19 protein, ECD, FITC Conjugated, His Tag (CAT#: V2LY-0526-LY2701)

Human Recombinant CD19 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2702)

Human Recombinant CD19, High activity protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2703)

Human Recombinant CD19 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2704)

Human Recombinant CD19 protein, ECD, His Tag (CAT#: V2LY-0526-LY2705)

Antibody Pairs

CD19 Matched Antibody Pair (207) (CAT#: APMAB-207LY)

CD19 Matched Antibody Pair (208) (CAT#: APMAB-208LY)

Related Products

Human Recombinant CD19 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2704)

Rat Anti-CD19 Recombinant Antibody (AG07) (CAT#: V2LY-0524-LY84)

Mouse Anti-CD19 Recombinant Antibody (
4C6G1) (CAT#: V2LY-0725-LY136)

Mouse Anti-CD19 Recombinant Antibody (PDR134) (CAT#: CBMAB-C0982-CQ)

Mouse Anti-CD19 Recombinant Antibody (CBXC-2489) (CAT#: CBMAB-C4481-CQ)

Cynomolgus, Rhesus Recombinant CD19 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY1686)

Mouse Recombinant CD19 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY7958)

Rabbit Anti-CD19 Recombinant Antibody (CBNH-019) (CAT#: CBMAB-R4344-CN)

Human Recombinant CD19 protein, ECD, FITC Conjugated, His Tag (CAT#: V2LY-0526-LY2701)

Rabbit Anti-CD19 Recombinant Antibody (
3D3) (CAT#: V2LY-0725-LY629)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry