Human Recombinant CD200 protein, ECD, Biotin Conjugated, His & AVI Tag (V2LY-0526-LY2731)

Name: Human Recombinant CD200 protein, ECD, Biotin Conjugated, His & AVI Tag
SKU: V2LY-0526-LY2731
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His & AVI Tag

Protein Construction

This product is Human Recombinant CD200 protein, ECD, Biotin Conjugated, His & AVI Tag consist of Amino Acid: 1-232 and predicts a molecular mass of 25.71 kDa.

Molecule Mass

25.71 kDa

Protein Domain

ECD

Verified

HPLC

Conjugates

Biotin

Sequence

Amino Acid: 1-232

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD200 Molecule

Alternative Names

CD200 Molecule; CD200 Antigen; Antigen Identified By Monoclonal Antibody MRC OX-2; MOX1; MOX2; OX-2 Membrane Glycoprotein; OX-2; MRC;

Function

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.

Biological Process

Cell-cell adhesion Source: ARUK-UCL
Heterotypic cell-cell adhesion Source: ARUK-UCL
Negative regulation of cell population proliferation Source: ARUK-UCL
Negative regulation of interleukin-6 production Source: ARUK-UCL
Negative regulation of leukocyte activation Source: ARUK-UCL
Negative regulation of macrophage activation Source: ARUK-UCL
Negative regulation of macrophage migration Source: ARUK-UCL
Negative regulation of matrix metallopeptidase secretion Source: ARUK-UCL
Negative regulation of neuroinflammatory response Source: ARUK-UCL
Negative regulation of neuron death Source: ARUK-UCL
Negative regulation of NF-kappaB transcription factor activity Source: ARUK-UCL
Negative regulation of T cell migration Source: ARUK-UCL
Positive regulation of arginase activity Source: ARUK-UCL
Positive regulation of CREB transcription factor activity Source: ARUK-UCL
Positive regulation of protein-glutamine gamma-glutamyltransferase activity Source: ARUK-UCL
Positive regulation of transforming growth factor beta production Source: ARUK-UCL
Regulation of immune response Source: Reactome
Regulation of neuroinflammatory response Source: ARUK-UCL

Cellular Location

Cell membrane

Topology

Extracellular: 31-232
Helical: 233-259
Cytoplasmic: 260-278

Liu, J. Q., Hu, A., Zhu, J., Yu, J., Talebian, F., & Bai, X. F. (2020). CD200-CD200R Pathway in the regulation of tumor immune microenvironment and immunotherapy. Tumor Microenvironment, 155-165.

Ismail, A. A., Donia, H. M., Ghatesh, H. M., & Farid, C. I. (2020). CD200/CD200 receptor axis in psoriasis vulgaris. PloS one, 15(3), e0230621.

Estep, R. D., Govindan, A. N., Fitzpatrick, K., Blair, T. C., Rezaee, S. R., Blackbourn, D. J., & Wong, S. W. (2020). Membrane-associated and secreted forms of the Rhesus macaque rhadinovirus-encoded CD200 homologue and cellular CD200 demonstrate differential effects on Rhesus Macaque CD200 Receptor signaling and regulation of myeloid cell activation. Journal of virology, 95(5), e01654-20.

Weng, L. C., Hou, S. H., Lei, S. T., Peng, H. Y., Li, M. Q., & Zhao, D. (2020). Estrogen-regulated CD200 inhibits macrophage phagocytosis in endometriosis. Journal of reproductive immunology, 138, 103090.

Manich, G., Recasens, M., Valente, T., Almolda, B., González, B., & Castellano, B. (2019). Role of the CD200-CD200R axis during homeostasis and neuroinflammation. Neuroscience, 405, 118-136.

Olin, M. R., Ampudia-Mesias, E., Pennell, C. A., Sarver, A., Chen, C. C., Moertel, C. L., ... & Pluhar, G. E. (2019). Treatment combining CD200 immune checkpoint inhibitor and tumor-lysate vaccination after surgery for pet dogs with high-grade glioma. Cancers, 11(2), 137.

Rexin, P., Tauchert, A., Haenze, J., Heers, H., Schmidt, A., Hofmann, R., & Hegele, A. (2018). The immune checkpoint molecule CD200 is associated with tumor grading and metastasis in bladder cancer. Anticancer research, 38(5), 2749-2754.

Chen, Z., Kapus, A., Khatri, I., Kos, O., Zhu, F., & Gorczynski, R. M. (2018). Cell membrane-bound CD200 signals both via an extracellular domain and following nuclear translocation of a cytoplasmic fragment. Leukemia research, 69, 72-80.

Nouri, F., Sadeghpour, H., Heidari, R., & Dehshahri, A. (2017). Preparation, characterization, and transfection efficiency of low molecular weight polyethylenimine-based nanoparticles for delivery of the plasmid encoding CD200 gene. International journal of nanomedicine, 12, 5557.

Gorczynski, R. M., & Zhu, F. (2017). Checkpoint blockade in solid tumors and B-cell malignancies, with special consideration of the role of CD200. Cancer management and research, 9, 601.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD200 protein, ECD, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2729)

Human Recombinant CD200 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2730)

Human Recombinant CD200 protein, His Tag (CAT#: V2LY-0526-LY2732)

Mouse Recombinant CD200 protein, ECD, His Tag (CAT#: V2LY-0526-LY7969)

Mouse Recombinant CD200 protein, ECD, Fc Tag (CAT#: V2LY-0526-LY7970)

Rhesus Recombinant CD200 protein, hFc Tag (CAT#: V2LY-0526-LY9668)

Rhesus Recombinant CD200 protein, His Tag (CAT#: V2LY-0526-LY9669)

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Human Recombinant CD200 protein, His Tag (CAT#: V2LY-0526-LY2732)

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Human Recombinant CD200 protein, ECD, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2729)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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