Human Recombinant CD5 protein, ECD, His Tag (V2LY-0526-LY2894)

Name: Human Recombinant CD5 protein, ECD, His Tag
SKU: V2LY-0526-LY2894
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CD5 protein, ECD, His Tag consist of Amino Acid: 1-372 and predicts a molecular mass of 40 kDa.

Molecule Mass

40 kDa

Protein Domain

ECD

Verified

HPLC

Sequence

Amino Acid: 1-372

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD5 Molecule

Alternative Names

CD5 Molecule; Lymphocyte Antigen T1/Leu-1; CD5 Antigen (P56-62); LEU1; T-Cell Surface Glycoprotein CD5; CD5 Antigen; T1;

Function

May act as a receptor in regulating T-cell proliferation.

Biological Process

Apoptotic signaling pathway Source: Ensembl
Cell recognition Source: UniProtKB
T cell costimulation Source: GO_Central

Cellular Location

Cell membrane

Topology

Extracellular: 25-372
Helical: 373-402
Cytoplasmic: 403-495

PTM

Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1.

Velasco-de-Andrés, M., Català, C., Casadó-Llombart, S., Simões, I., Zaragoza, O., Carreras, E., & Lozano, F. (2021). The lymphocyte scavenger receptor CD5 plays a nonredundant role in fungal infection. Cellular & molecular immunology, 18(2), 498-500.

Dai, Z., Mu, W., Zhao, Y., Jia, X., Liu, J., Wei, Q., ... & Zhou, J. (2021). The rational development of CD5-targeting biepitopic CARs with fully human heavy-chain-only antigen recognition domains. Molecular Therapy, 29(9), 2707-2722.

Matson, C. A., Choi, S., Livak, F., Zhao, B., Mitra, A., Love, P. E., & Singh, N. J. (2020). CD5 dynamically calibrates basal NF-κB signaling in T cells during thymic development and peripheral activation. Proceedings of the National Academy of Sciences, 117(25), 14342-14353.

Velasco-de Andrés, M., Casadó-Llombart, S., Català, C., Leyton-Pereira, A., Lozano, F., & Aranda, F. (2020). Soluble CD5 and CD6: Lymphocytic Class I Scavenger Receptors as Immunotherapeutic Agents. Cells, 9(12), 2589.

Blaize, G., Daniels-Treffandier, H., Aloulou, M., Rouquié, N., Yang, C., Marcellin, M., ... & Lesourne, R. (2020). CD5 signalosome coordinates antagonist TCR signals to control the generation of Treg cells induced by foreign antigens. Proceedings of the National Academy of Sciences, 117(23), 12969-12979.

Alotaibi, F., Rytelewski, M., Figueredo, R., Zareardalan, R., Zhang, M., Ferguson, P. J., ... & Koropatnick, J. (2020). CD5 blockade enhances ex vivo CD8+ T cell activation and tumour cell cytotoxicity. European journal of immunology, 50(5), 695-704.

Burgueño‐Bucio, E., Mier‐Aguilar, C. A., & Soldevila, G. (2019). The multiple faces of CD5. Journal of leukocyte biology, 105(5), 891-904.

Zhao, P., Li, L., Zhou, S., Qiu, L., Qian, Z., Liu, X., ... & Zhang, H. (2019). CD5 expression correlates with inferior survival and enhances the negative effect of p53 overexpression in diffuse large B‐cell lymphoma. Hematological oncology, 37(4), 360-367.

Voisinne, G., Gonzalez de Peredo, A., & Roncagalli, R. (2018). CD5, an undercover regulator of TCR signaling. Frontiers in immunology, 9, 2900.

Abós, B., Bird, S., Granja, A. G., Morel, E., Bayona, J. A. M., Barreda, D. R., & Tafalla, C. (2018). Identification of the first teleost CD5 molecule: additional evidence on phenotypical and functional similarities between fish IgM+ B cells and mammalian B1 cells. The Journal of Immunology, 201(2), 465-480.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant CD5 protein, His Tag (CAT#: V2LY-0526-LY1441)

Human Recombinant CD5 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY2892)

Human Recombinant CD5 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2893)

Human Recombinant CD5 protein, His Tag (CAT#: V2LY-0526-LY2895)

Human Recombinant CD5 protein, AF488 Conjugated, His Tag (CAT#: V2LY-0526-LY2896)

Human Recombinant CD5 protein, AF647 Conjugated, His Tag (CAT#: V2LY-0526-LY2897)

Human Recombinant CD5 protein, APC Conjugated, His Tag (CAT#: V2LY-0526-LY2898)

Mouse Recombinant CD5 protein, His Tag (CAT#: V2LY-0526-LY8040)

Rat Recombinant CD5 protein, hFc Tag (CAT#: V2LY-0526-LY9251)

Rat Recombinant CD5 protein, His Tag (CAT#: V2LY-0526-LY9252)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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