Human Recombinant CD84 protein, His Tag (V2LY-0526-LY2968)

Name: Human Recombinant CD84 protein, His Tag
SKU: V2LY-0526-LY2968
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CD84 protein, His Tag consist of Amino Acid: 1-225 and predicts a molecular mass of 24 kDa.

Molecule Mass

24 kDa

Sequence

Amino Acid: 1-225

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD84 Molecule

Function

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Can mediate natural killer (NK) cell cytotoxicity dependent on SH2D1A and SH2D1B (By similarity).
Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seem be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A-dependent pathway. May serve as a marker for hematopoietic progenitor cells (PubMed:11564780, PubMed:12115647. PubMed:12928397, PubMed:12962726, PubMed:16037392) Required for a prolonged T-cell:B-cell contact, optimal T follicular helper function, and germinal center formation. In germinal centers involved in maintaining B-cell tolerance and in preventing autoimmunity (By similarity).
In mast cells negatively regulates high affinity immunoglobulin epsilon receptor signaling; independent of SH2D1A and SH2D1B but implicating FES and PTPN6/SHP-1 (PubMed:22068234).
In macrophages enhances LPS-induced MAPK phosphorylation and NF-kappaB activation and modulates LPS-induced cytokine secretion; involving ITSM 2 (By similarity).
Positively regulates macroautophagy in primary dendritic cells via stabilization of IRF8; inhibits TRIM21-mediated proteasomal degradation of IRF8 (PubMed:29434592).

Biological Process

Adaptive immune response Source: UniProtKB-KW
Autophagy Source: UniProtKB-KW
Defense response Source: ProtInc
Homophilic cell adhesion via plasma membrane adhesion molecules Source: ProtInc
Innate immune response Source: UniProtKB-KW
Leukocyte migration Source: Reactome
Negative regulation of granulocyte macrophage colony-stimulating factor production Source: UniProtKB
Negative regulation of interleukin-18 production Source: UniProtKB
Negative regulation of mast cell activation Source: UniProtKB
Negative regulation of mast cell degranulation Source: UniProtKB
Regulation of store-operated calcium entry Source: UniProtKB

Cellular Location

Cell membrane

Topology

Extracellular: 22-225
Helical: 226-246
Cytoplasmic: 247-345

PTM

Phosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A is dependent of tyrosine phosphorylation of its cytoplasmic domain. Phosphorylated on Tyr-296 and Tyr-316 following platelet aggregation. Phosphorylated on tyrosine residues upon high affinity immunoglobulin epsilon receptor aggregation in mast cells.
N-glycosylated.

Tong, D., Zhao, Y., Tang, Y., Ma, J., Wang, Z., & Li, C. (2021). Circ-Usp10 promotes microglial activation and induces neuronal death by targeting miRNA-152-5p/CD84. Bioengineered, (just-accepted).

Lewinsky, H., Gunes, E. G., David, K., Radomir, L., Kramer, M. P., Pellegrino, B., ... & Shachar, I. (2021). CD84 is a regulator of the immunosuppressive microenvironment in multiple myeloma. JCI insight, 6(4).

Schuhmann, M. K., Stoll, G., Bieber, M., Vögtle, T., Hofmann, S., Klaus, V., ... & Stegner, D. (2020). CD84 links T cell and platelet activity in cerebral thrombo-inflammation in acute stroke. Circulation research, 127(8), 1023-1035.

Barak, A. F., Lewinsky, H., Perpinial, M., Huber, V., Radomir, L., Kramer, M. P., ... & Shachar, I. (2020). Bone marrow dendritic cells support the survival of chronic lymphocytic leukemia cells in a CD84 dependent manner. Oncogene, 39(9), 1997-2008.

Cuenca, M., Sintes, J., Lányi, Á., & Engel, P. (2019). CD84 cell surface signaling molecule: An emerging biomarker and target for cancer and autoimmune disorders. Clinical Immunology, 204, 43-49.

Lewinsky, H., Barak, A. F., Huber, V., Kramer, M. P., Radomir, L., Sever, L., ... & Shachar, I. (2018). CD84 regulates PD-1/PD-L1 expression and function in chronic lymphocytic leukemia. The Journal of clinical investigation, 128(12), 5465-5478.

Wang, T. T., Pham, Q., & Kim, Y. S. (2018). Elucidating the role of CD84 and AHR in modulation of LPS-induced cytokines production by cruciferous vegetable-derived compounds indole-3-carbinol and 3, 3′-diindolylmethane. International journal of molecular sciences, 19(2), 339.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant CD84 protein, hFc Tag (CAT#: V2LY-0526-LY1454)

Cynomolgus, Rhesus Recombinant CD84 protein, His Tag (CAT#: V2LY-0526-LY1696)

Mouse Recombinant CD84 protein, His Tag (CAT#: V2LY-0526-LY8078)

Human CD84 ELISA Kit (CAT#: V2LY-0626-LY5253)

Related Products

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Human CD84 ELISA Kit (CAT#: V2LY-0626-LY5253)

Mouse Anti-CD84 Recombinant Antibody (CBXC-0275) (CAT#: CBMAB-C5866-CQ)

Cynomolgus, Rhesus Recombinant CD84 protein, His Tag (CAT#: V2LY-0526-LY1696)

Mouse Recombinant CD84 protein, His Tag (CAT#: V2LY-0526-LY8078)

Rabbit Anti-CD84 Recombinant Antibody (4) (CAT#: CBMAB-C1508-LY)

Cynomolgus Recombinant CD84 protein, hFc Tag (CAT#: V2LY-0526-LY1454)

Mouse Anti-CD84 Recombinant Antibody (CBT3706) (CAT#: V2LY-0625-LY874)

Mouse Anti-CD84 Recombinant Antibody (CD84.1.21) (CAT#: CBMAB-C0540-CQ)

Isotype control

For research use only. Not intended for any clinical use.

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