Human Recombinant CD96 protein, His Tag (V2LY-0526-LY2980)

Name: Human Recombinant CD96 protein, His Tag
SKU: V2LY-0526-LY2980
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CD96 protein, His Tag consist of Amino Acid: 1-503 and predicts a molecular mass of 55 kDa.

Molecule Mass

55 kDa

Verified

HPLC

Sequence

Amino Acid: 1-503

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD96 Molecule

Alternative Names

CD96 Molecule; T Cell-Activated Increased Late Expression Protein; Cell Surface Antigen CD96; CD96 Antigen; T Cell Activation, Increased Late Expression; T-Cell Surface Protein Tactile; TACTILE;

Function

May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.

Biological Process

Cell adhesion Source: ProtInc
Cell-matrix adhesion Source: MGI
Immune response Source: ProtInc
Inflammatory response Source: GO_Central
Negative regulation of interferon-gamma production Source: Ensembl
Negative regulation of natural killer cell cytokine production Source: Ensembl
Regulation of immune response Source: Reactome
Response to lipopolysaccharide Source: Ensembl

Cellular Location

Membrane

Involvement in disease

C syndrome (CSYN): The disease is caused by variants affecting the gene represented in this entry. A chromosomal aberration involving CD96 has been found in a patient with C syndrome. Translocation t(3;18)(q13.13;q12.1). CD96 gene was located at the 3q13.13 breakpoint. Precise structural analysis around the breakpoint showed that the gene was disrupted by the translocation in exon 5, probably leading to premature termination or loss of expression of CD96 protein. No gene was detected at the chromosome 18 breakpoint.
A syndrome characterized by trigonocephaly, severe mental retardation, hypotonia, variable cardiac defects, redundant skin, and dysmorphic facial features, including upslanted palpebral fissures, epicanthal folds, depressed nasal bridge, and low-set, posteriorly rotated ears.

Topology

Extracellular: 22-519
Helical: 520-540
Cytoplasmic: 541-585

Lee, P. S., Chau, B., Barman, I., Bee, C., Jashnani, A., Hogan, J. M., ... & Strop, P. (2021, January). Antibody blockade of CD96 by distinct molecular mechanisms. In Mabs (Vol. 13, No. 1, p. 1979800). Taylor & Francis.

Xie, C., Wang, Z., Li, Y., Wu, F., Xia, H., Lu, Y., ... & Kwok, K. W. (2021). Molecular characterization and expression of CD96 in Nile tilapia (Oreochromis niloticus) in response to different pathogens stimulus. Aquaculture Reports, 20, 100705.

Zhang, H., Liu, Q., Lei, Y., Zhou, J., Jiang, W., Cui, Y., ... & Ke, Z. (2021). Direct interaction between CD155 and CD96 promotes immunosuppression in lung adenocarcinoma. Cellular & molecular immunology, 18(6), 1575-1577.

Maas, R. J., Hoogstad-van Evert, J. S., Van der Meer, J. M., Mekers, V., Rezaeifard, S., Korman, A. J., ... & Dolstra, H. (2020). TIGIT blockade enhances functionality of peritoneal NK cells with altered expression of DNAM-1/TIGIT/CD96 checkpoint molecules in ovarian cancer. Oncoimmunology, 9(1), 1843247.

Liu, F., Huang, J., He, F., Ma, X., Fan, F., Meng, M., ... & Zhang, L. (2020). CD96, a new immune checkpoint, correlates with immune profile and clinical outcome of glioma. Scientific reports, 10(1), 1-10.

Chiang, E. Y., de Almeida, P. E., de Almeida Nagata, D. E., Bowles, K. H., Du, X., Chitre, A. S., ... & Grogan, J. L. (2020). CD96 functions as a co‐stimulatory receptor to enhance CD8+ T cell activation and effector responses. European journal of immunology, 50(6), 891-902.

Sun, H., Huang, Q., Huang, M., Wen, H., Lin, R., Zheng, M., ... & Sun, C. (2019). Human CD96 correlates to natural killer cell exhaustion and predicts the prognosis of human hepatocellular carcinoma. Hepatology, 70(1), 168-183.

Deuss, F. A., Watson, G. M., Fu, Z., Rossjohn, J., & Berry, R. (2019). Structural basis for CD96 immune receptor recognition of nectin-like protein-5, CD155. Structure, 27(2), 219-228.

Georgiev, H., Ravens, I., Papadogianni, G., & Bernhardt, G. (2018). Coming of age: CD96 emerges as modulator of immune responses. Frontiers in immunology, 9, 1072.

Roman Aguilera, A., Lutzky, V. P., Mittal, D., Li, X. Y., Stannard, K., Takeda, K., ... & Smyth, M. J. (2018). CD96 targeted antibodies need not block CD96-CD155 interactions to promote NK cell anti-metastatic activity. Oncoimmunology, 7(5), e1424677.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant CD96 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY1458)

Cynomolgus Recombinant CD96 protein, His Tag (CAT#: V2LY-0526-LY1459)

Human Recombinant CD96 protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2978)

Human Recombinant CD96 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY2979)

Mouse Recombinant CD96 protein, His Tag (CAT#: V2LY-0526-LY8081)

CD96 Matched Antibody Pair (240) (CAT#: APMAB-240LY)

Antibody Pairs

CD96 Matched Antibody Pair (240) (CAT#: APMAB-240LY)

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CD96 Matched Antibody Pair (240) (CAT#: APMAB-240LY)

Human Recombinant CD96 protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2978)

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Human Recombinant CD96 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY2979)

Rabbit Anti-CD96 Recombinant Antibody (CBR065G) (CAT#: CBMAB-BT026LY)

Mouse Recombinant CD96 protein, His Tag (CAT#: V2LY-0526-LY8081)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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