Human Recombinant CDC42BPA, Active protein, GST Tag (V2LY-0526-LY5636)

Name: Human Recombinant CDC42BPA, Active protein, GST Tag
SKU: V2LY-0526-LY5636
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant CDC42BPA, Active protein, GST Tag consist of Amino Acid: 1-473 and predicts a molecular mass of 73 kDa.

Molecule Mass

73 kDa

Sequence

Amino Acid: 1-473

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

CDC42 Binding Protein Kinase Alpha

Function

Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration (PubMed:15723050, PubMed:9418861, PubMed:9092543).
Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2 (PubMed:21457715).
In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (PubMed:18854160).
Phosphorylates: PPP1R12A, LIMK1 and LIMK2 (PubMed:11340065, PubMed:11399775).
May play a role in TFRC-mediated iron uptake (PubMed:20188707).
In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity).
Triggers the formation of an extrusion apical actin ring required for epithelial extrusion of apoptotic cells (PubMed:29162624).

Biological Process

Actin cytoskeleton reorganization Source: UniProtKB
Actomyosin structure organization Source: UniProtKB
Cell migration Source: UniProtKB
Intracellular signal transduction Source: InterPro
Peptidyl-threonine phosphorylation Source: GO_Central
Protein phosphorylation Source: UniProtKB

Cellular Location

Cytoplasm; Lamellipodium. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent. Localizes in the lamellipodium in a FAM89B/LRAP25-dependent manner.

PTM

Proteolytically cleaved by caspases upon apoptosis induction. The cleavage at Asp-478 by CASP3 increases its kinase activity (in vitro).

Richard, C., Viret, S., Cantero Aguilar, L., Lefevre, C., Leduc, M., Faouzi, E. H., ... & Verdier, F. (2021). Myotonic dystrophy kinase‐related CDC42‐binding kinase α, a new transferrin receptor type 2‐binding partner, is a regulator of erythropoiesis. American Journal of Hematology, 96(4), 480-492.

Hu, H. F., Xu, W. W., Wang, Y., Zheng, C. C., Zhang, W. X., Li, B., & He, Q. Y. (2018). Comparative proteomics analysis identifies Cdc42-Cdc42BPA signaling as prognostic biomarker and therapeutic target for colon cancer invasion. Journal of proteome research, 17(1), 265-275.

He, P. Y., Yip, W. K., Chai, B. L., Chai, B. Y., Jabar, M. F., Dusa, N., ... & Seow, H. F. (2017). Inhibition of cell migration and invasion by miR‑29a‑3p in a colorectal cancer cell line through suppression of CDC42BPA mRNA expression. Oncology reports, 38(6), 3554-3566.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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