Human Recombinant CES1 protein, His Tag (V2LY-0526-LY2569)

Name: Human Recombinant CES1 protein, His Tag
SKU: V2LY-0526-LY2569
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CES1 protein, His Tag consist of Amino Acid: 1-567 and predicts a molecular mass of 62.08 kDa.

Molecule Mass

62.08 kDa

Verified

HPLC

Sequence

Amino Acid: 1-567

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥85% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Carboxylesterase 1

Function

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (PubMed:7980644, PubMed:9169443, PubMed:9490062, PubMed:18762277).
Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester (PubMed:7980644, PubMed:9169443, PubMed:9490062, PubMed:18762277).
Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine (PubMed:7980644).
Catalyzes the transesterification of cocaine to form cocaethylene (PubMed:7980644).
Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate (PubMed:7980644).
Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins (PubMed:21049984).
Hydrolyzes cellular cholesteryl esters to free cholesterols and promotes reverse cholesterol transport (RCT) by facilitating both the initial and final steps in the process (PubMed:18762277, PubMed:16024911, PubMed:11015575, PubMed:16971496).
First of all, allows free cholesterol efflux from macrophages to extracellular cholesterol acceptors and secondly, releases free cholesterol from lipoprotein-delivered cholesteryl esters in the liver for bile acid synthesis or direct secretion into the bile (PubMed:18762277, PubMed:18599737, PubMed:16971496).

Biological Process

Angiotensin maturation Source: Reactome
Cellular response to cholesterol Source: ARUK-UCL
Cellular response to low-density lipoprotein particle stimulus Source: ARUK-UCL
Cholesterol biosynthetic process Source: BHF-UCL
Cholesterol ester hydrolysis involved in cholesterol transport Source: BHF-UCL
Cholesterol homeostasis Source: ARUK-UCL
Cholesterol metabolic process Source: UniProtKB
Epithelial cell differentiation Source: UniProtKB
Lipid catabolic process Source: GO_Central
Medium-chain fatty acid metabolic process Source: BHF-UCL
Negative regulation of cholesterol storage Source: UniProtKB
Positive regulation of cholesterol efflux Source: UniProtKB
Positive regulation of cholesterol metabolic process Source: UniProtKB
Regulation of bile acid biosynthetic process Source: UniProtKB
Regulation of bile acid secretion Source: UniProtKB
Response to toxic substance Source: ProtInc
Reverse cholesterol transport Source: UniProtKB
Xenobiotic metabolic process Source: Reactome

Cellular Location

Cytoplasm; Endoplasmic reticulum lumen; Lipid droplet. Moves from cytoplasm to lipid droplets upon lipid loading. Associates with lipid droplets independently of triglycerides (TG) content of the droplets and hydrolyzes cholesteryl esters more efficiently from mixed droplets.

PTM

Contains sialic acid.
Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19.

Na, K., Kim, M., Kim, C. Y., Lim, J. S., Cho, J. Y., Shin, H., ... & Paik, Y. K. (2020). Potential Regulatory Role of Human-Carboxylesterase-1 Glycosylation in Liver Cancer Cell Growth. Journal of Proteome Research, 19(12), 4867-4883.

Her, L., & Zhu, H. J. (2020). Carboxylesterase 1 and precision pharmacotherapy: pharmacogenetics and nongenetic regulators. Drug Metabolism and Disposition, 48(3), 230-244.

Xu, Y., Zhu, Y., Bawa, F. C., Hu, S., Pan, X., Yin, L., & Zhang, Y. (2020). Hepatocyte‐Specific Expression of Human Carboxylesterase 1 Attenuates Diet‐Induced Steatohepatitis and Hyperlipidemia in Mice. Hepatology communications, 4(4), 527-539.

Wang, X., Shi, J., & Zhu, H. J. (2019). Functional study of carboxylesterase 1 protein isoforms. Proteomics, 19(4), 1800288.

Ding, L., Tian, Z., Hou, J., Dou, T., Jin, Q., Wang, D., ... & Ge, G. (2019). Sensing carboxylesterase 1 in living systems by a practical and isoform-specific fluorescent probe. Chinese Chemical Letters, 30(3), 558-562.

Tian, Z., Ding, L., Li, K., Song, Y., Dou, T., Hou, J., ... & Cui, J. (2019). Rational design of a long-wavelength fluorescent probe for highly selective sensing of carboxylesterase 1 in living systems. Analytical chemistry, 91(9), 5638-5645.

Qian, Y., Wang, X., & Markowitz, J. S. (2019). In vitro inhibition of carboxylesterase 1 by major cannabinoids and selected metabolites. Drug Metabolism and Disposition, 47(5), 465-472.

Neuvonen, M., Tarkiainen, E. K., Tornio, A., Hirvensalo, P., Tapaninen, T., Paile‐Hyvärinen, M., ... & Niemi, M. (2018). Effects of genetic variants on carboxylesterase 1 gene expression, and clopidogrel pharmacokinetics and antiplatelet effects. Basic & clinical pharmacology & toxicology, 122(3), 341-345.

Xu, J., Xu, Y., Xu, Y., Yin, L., & Zhang, Y. (2017). Global inactivation of carboxylesterase 1 (Ces1/Ces1g) protects against atherosclerosis in Ldlr−/− mice. Scientific reports, 7(1), 1-12.

Wang, X., Rida, N., Shi, J., Wu, A. H., Bleske, B. E., & Zhu, H. J. (2017). A comprehensive functional assessment of carboxylesterase 1 nonsynonymous polymorphisms. Drug Metabolism and Disposition, 45(11), 1149-1155.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant CES1 protein, His Tag (CAT#: V2LY-0526-LY7912)

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Isotype control

For research use only. Not intended for any clinical use.

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