Human Recombinant CFL2 protein, His Tag (V2LY-0526-LY3310)

Name: Human Recombinant CFL2 protein, His Tag
SKU: V2LY-0526-LY3310
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CFL2 protein, His Tag consist of Amino Acid: 2-166 and predicts a molecular mass of 20.4 kDa.

Molecule Mass

20.4 kDa

Sequence

Amino Acid: 2-166

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

cofilin 2

Function

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3 (PubMed:19752190).
It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere (By similarity).

Biological Process

Actin filament depolymerization Source: UniProtKB
Actin filament fragmentation Source: GO_Central
Actin filament severing Source: GO_Central
Cell motility Source: GO_Central
Muscle cell cellular homeostasis Source: Ensembl
Positive regulation of actin filament depolymerization Source: UniProtKB
Sarcomere organization Source: Ensembl
Skeletal muscle tissue development Source: Ensembl

Cellular Location

Cytoskeleton; Nucleus matrix. Colocalizes with CSPR3 in the Z line of sarcomeres.

Involvement in disease

Nemaline myopathy 7 (NEM7): A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-like or rod-shaped structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved.

PTM

The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.

Zhu, Z., Duan, P., Song, H., Zhou, R., & Chen, T. (2021). Downregulation of Circular RNA PSEN1 ameliorates ferroptosis of the high glucose treated retinal pigment epithelial cells via miR-200b-3p/cofilin-2 axis. Bioengineered, 12(2), 12555-12567.

Mamoor, S. (2021). Differential expression of cofilin 2 in cancers of the breast.

Pignataro, M., Di Rocco, G., Lancellotti, L., Bernini, F., Subramanian, K., Castellini, E., ... & Del Monte, F. (2020). Phosphorylated cofilin-2 is more prone to oxidative modifications on Cys39 and favors amyloid fibril formation. Redox biology, 37, 101691.

Wo, Q., Zhang, D., Hu, L., Lyu, J., Xiang, F., Zheng, W., ... & Qi, X. (2019). Long noncoding RNA SOX2-OT facilitates prostate cancer cell proliferation and migration via miR-369-3p/CFL2 axis. Biochemical and biophysical research communications, 520(3), 586-593.

Mohri, K., Suzuki-Toyota, F., Obinata, T., & Sato, N. (2019). Chimeric Mice with Deletion of Cfl2 that Encodes Muscle-Type Cofilin (MCF or Cofilin-2) Results in Defects of Striated Muscles, Both Skeletal and Cardiac Muscles. Zoological science, 36(2), 112-119.

Zhu, H., Yang, H., Zhao, S., Zhang, J., Liu, D., Tian, Y., ... & Su, Y. (2018). Role of the cofilin 2 gene in regulating the myosin heavy chain genes in mouse myoblast C2C12 cells. International journal of molecular medicine, 41(2), 1096-1102.

Yu, B. B., Lin, G. X., Li, L., Qu, S., Liang, Z. G., Chen, K. H., ... & Zhu, X. D. (2018). Cofilin-2 acts as a marker for predicting radiotherapy response and is a potential therapeutic target in nasopharyngeal carcinoma. Medical science monitor: international medical journal of experimental and clinical research, 24, 2317.

Yehl, J., Kudryashova, E., Reisler, E., Kudryashov, D., & Polenova, T. (2017). Structural analysis of human cofilin 2/filamentous actin assemblies: atomic-resolution insights from magic angle spinning NMR spectroscopy. Scientific reports, 7(1), 1-10.

Rangrez, A. Y., Hoppe, P., Kuhn, C., Zille, E., Frank, J., Frey, N., & Frank, D. (2017). MicroRNA miR-301a is a novel cardiac regulator of Cofilin-2. PloS one, 12(9), e0183901.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human CFL2 ELISA Kit (CAT#: V2LY-0626-LY1082)

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Human CFL2 ELISA Kit (CAT#: V2LY-0626-LY1082)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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