Mouse Anti-CFL2 Recombinant Antibody (BR133) (CBMAB-BR133LY)

Name: Mouse Anti-CFL2 Recombinant Antibody (BR133)
SKU: CBMAB-BR133LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

BR133

Application

FC, IF, IHC-P, WB

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Cofilin 2/CFL2.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	0.1-0.5 μg/ml
FC	1-3 μg/10^6 cells
IF(ICC)	2 μg/ml
IHC-P	0.5-1 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

4mg Trehalose

Preservative

0.05 mg sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

cofilin 2

Entrez Gene ID

Human	1073
Mouse	12632
Rat	366624

UniProt ID

Human	Q9Y281
Mouse	P45591
Rat	M0RC65

Alternative Names

Cofilin-2; Cofilin, muscle isoform; CFL2

Function

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3 (PubMed:19752190).
It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere (By similarity).

Biological Process

Actin filament depolymerization Source: UniProtKB
Actin filament fragmentation Source: GO_Central
Actin filament severing Source: GO_Central
Cell motility Source: GO_Central
Muscle cell cellular homeostasis Source: Ensembl
Positive regulation of actin filament depolymerization Source: UniProtKB
Sarcomere organization Source: Ensembl
Skeletal muscle tissue development Source: Ensembl

Cellular Location

Cytoskeleton; Nucleus matrix. Colocalizes with CSPR3 in the Z line of sarcomeres.

Involvement in disease

Nemaline myopathy 7 (NEM7): A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-like or rod-shaped structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved.

PTM

The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.

Zhu, Z., Duan, P., Song, H., Zhou, R., & Chen, T. (2021). Downregulation of Circular RNA PSEN1 ameliorates ferroptosis of the high glucose treated retinal pigment epithelial cells via miR-200b-3p/cofilin-2 axis. Bioengineered, 12(2), 12555-12567.

Mamoor, S. (2021). Differential expression of cofilin 2 in cancers of the breast.

Pignataro, M., Di Rocco, G., Lancellotti, L., Bernini, F., Subramanian, K., Castellini, E., ... & Del Monte, F. (2020). Phosphorylated cofilin-2 is more prone to oxidative modifications on Cys39 and favors amyloid fibril formation. Redox biology, 37, 101691.

Wo, Q., Zhang, D., Hu, L., Lyu, J., Xiang, F., Zheng, W., ... & Qi, X. (2019). Long noncoding RNA SOX2-OT facilitates prostate cancer cell proliferation and migration via miR-369-3p/CFL2 axis. Biochemical and biophysical research communications, 520(3), 586-593.

Mohri, K., Suzuki-Toyota, F., Obinata, T., & Sato, N. (2019). Chimeric Mice with Deletion of Cfl2 that Encodes Muscle-Type Cofilin (MCF or Cofilin-2) Results in Defects of Striated Muscles, Both Skeletal and Cardiac Muscles. Zoological science, 36(2), 112-119.

Zhu, H., Yang, H., Zhao, S., Zhang, J., Liu, D., Tian, Y., ... & Su, Y. (2018). Role of the cofilin 2 gene in regulating the myosin heavy chain genes in mouse myoblast C2C12 cells. International journal of molecular medicine, 41(2), 1096-1102.

Yu, B. B., Lin, G. X., Li, L., Qu, S., Liang, Z. G., Chen, K. H., ... & Zhu, X. D. (2018). Cofilin-2 acts as a marker for predicting radiotherapy response and is a potential therapeutic target in nasopharyngeal carcinoma. Medical science monitor: international medical journal of experimental and clinical research, 24, 2317.

Yehl, J., Kudryashova, E., Reisler, E., Kudryashov, D., & Polenova, T. (2017). Structural analysis of human cofilin 2/filamentous actin assemblies: atomic-resolution insights from magic angle spinning NMR spectroscopy. Scientific reports, 7(1), 1-10.

Rangrez, A. Y., Hoppe, P., Kuhn, C., Zille, E., Frank, J., Frey, N., & Frank, D. (2017). MicroRNA miR-301a is a novel cardiac regulator of Cofilin-2. PloS one, 12(9), e0183901.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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