Human Recombinant CHN1 protein (V2LY-0526-LY3124)

Name: Human Recombinant CHN1 protein
SKU: V2LY-0526-LY3124
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Protein Construction

This product is Human Recombinant CHN1 protein consist of Amino Acid: 2-459 and predicts a molecular mass of 53.2 kDa.

Molecule Mass

53.2 kDa

Sequence

Amino Acid: 2-459

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Chimerin 1

Function

GTPase-activating protein for p21-rac and a phorbol ester receptor. Involved in the assembly of neuronal locomotor circuits as a direct effector of EPHA4 in axon guidance.

Biological Process

Ephrin receptor signaling pathway Source: UniProtKB
Intracellular signal transduction Source: InterPro
Motor neuron axon guidance Source: UniProtKB
Regulation of axonogenesis Source: UniProtKB
Regulation of GTPase activity Source: GO_Central
Regulation of small GTPase mediated signal transduction Source: Reactome

Cellular Location

Cytosol

Involvement in disease

Duane retraction syndrome 2 (DURS2): A form of Duane retraction syndrome, a congenital eye movement disorder characterized by a failure of cranial nerve VI (the abducens nerve) to develop normally, resulting in restriction or absence of abduction, adduction or both, narrowing of the palpebral fissure, and retraction of the globe on attempted adduction. Undiagnosed in children, it can lead to amblyopia, a permanent uncorrectable loss of vision.

PTM

Phosphorylated. Phosphorylation is EPHA4 kinase activity-dependent (By similarity).

Li, J. P., Zeng, S. H., Zhang, Y. H., & Liu, Y. J. (2021). Bioinformatics-based analysis of the association between the A1-chimaerin (CHN1) gene and gastric cancer. Bioengineered, 12(1), 2874-2889.

Zhao, H., Wang, L., Wang, S., Chen, X., Liang, M., Zhang, X., ... & Xu, X. (2021). CHN1 promotes epithelial–mesenchymal transition via the Akt/GSK-3β/Snail pathway in cervical carcinoma. Journal of Translational Medicine, 19(1), 1-12.

Zhou, T. C., Duan, W. H., Fu, X. L., Zhu, Q., Guo, L. Y., Zhou, Y., ... & Hu, M. (2020). Identification of a novel CHN1 p.(Phe213Val) variant in a large Han Chinese family with congenital Duane retraction syndrome. Scientific reports, 10(1), 1-9.

Liu, J., Li, Y., Chen, X., Xu, X., Zhao, H., Wang, S., ... & Wang, J. (2020). Upregulation of miR-205 induces CHN1 expression, which is associated with the aggressive behaviour of cervical cancer cells and correlated with lymph node metastasis. BMC cancer, 20(1), 1-13.

Biler, E. D., Ilim, O., Onay, H., & Uretmen, O. (2017). CHN1 gene mutation analysis in patients with Duane retraction syndrome. Journal of American Association for Pediatric Ophthalmology and Strabismus, 21(6), 472-475.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CHN1 protein, GST Tag (CAT#: V2LY-0526-LY3125)

CHN1 Matched Antibody Pair (270) (CAT#: APMAB-270LY)

Antibody Pairs

CHN1 Matched Antibody Pair (270) (CAT#: APMAB-270LY)

Related Products

Mouse Anti-CHN1 Recombinant Antibody (CBFYC-1843) (CAT#: CBMAB-C1907-FY)

Mouse Anti-CHN1 Recombinant Antibody (4D1) (CAT#: CBMAB-C6759-LY)

Mouse Anti-CHN1 Recombinant Antibody (3A3) (CAT#: CBMAB-C5643-LY)

CHN1 Matched Antibody Pair (270) (CAT#: APMAB-270LY)

Human Recombinant CHN1 protein, GST Tag (CAT#: V2LY-0526-LY3125)

Mouse Anti-CHN1 Recombinant Antibody (2A1) (CAT#: CBMAB-C4564-LY)

Mouse Anti-CHN1 Recombinant Antibody (3F8) (CAT#: CBMAB-C6071-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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