Human Recombinant CHRNB3 protein, ECD, His Tag (V2LY-0526-LY3131)

Name: Human Recombinant CHRNB3 protein, ECD, His Tag
SKU: V2LY-0526-LY3131
Rating: 5 (1 reviews)

See all CHRNB3 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CHRNB3 protein, ECD, His Tag consist of Amino Acid: 1-232 and predicts a molecular mass of 25.7 kDa.

Molecule Mass

25.7 kDa

Protein Domain

ECD

Sequence

Amino Acid: 1-232

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glycerol, Urea

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Cholinergic Receptor Nicotinic Beta 3 Subunit

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Biological Process

Chemical synaptic transmission Source: GO_Central
Ion transmembrane transport Source: GO_Central
Nervous system process Source: GO_Central
Regulation of membrane potential Source: GO_Central
Response to nicotine Source: GO_Central
Signal transduction Source: GO_Central
Synaptic transmission, cholinergic Source: GO_Central

Cellular Location

Postsynaptic cell membrane; Cell membrane

Topology

Extracellular: 25-232
Helical: 233-257
Helical: 265-282
Helical: 299-320
Cytoplasmic: 321-428
Helical: 429-447

Shi, J., Chen, N., Wang, Z., Wang, F., Tan, Y., Tan, S., ... & Luo, X. (2021). Cholinergic receptor nicotinic beta 3 subunit polymorphisms and smoking in male Chinese patients with schizophrenia. EC psychology and psychiatry, 10(7), 11.

Drayson, L. E., & Triplett, J. W. (2019). A Chrnb3‐Cre BAC transgenic mouse line for manipulation of gene expression in retinal ganglion cells. genesis, 57(9), e23305.

Zhao, L., Zou, L. Y., Cheng, B. F., Yu, X. J., Zou, J. H., & Han, W. (2019). Chronic Obstructive Pulmonary Disease Risk and Smoking Cessation Changes Induced by CHRNA5-A3 and CHRNB3-A6 Variation in a Chinese Male Population. Balkan journal of medical genetics: BJMG, 22(2), 51.

Li, M. D. (2018). Contribution of Variants in CHRNB3/A6 Gene Cluster on Chromosome 8 to Smoking Dependence. In Tobacco Smoking Addiction: Epidemiology, Genetics, Mechanisms, and Treatment (pp. 71-93). Springer, Singapore.

Wen, L., Han, H., Liu, Q., Su, K., Yang, Z., Cui, W., ... & Li, M. D. (2017). Significant association of the CHRNB3-CHRNA6 gene cluster with nicotine dependence in the Chinese Han population. Scientific reports, 7(1), 1-10.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant CHRNB3 protein, ECD, His Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CHRNB3 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY3130)

Related Products

Human Recombinant CHRNB3 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY3130)

Mouse Anti-CHRNB3 Recombinant Antibody (3G6) (CAT#: CBMAB-C6169-LY)

Mouse Anti-CHRNB3 Recombinant Antibody (CBT3521) (CAT#: V2LY-0625-LY1017)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry