Human Recombinant CHST15 protein, His Tag (V2LY-0526-LY3133)

Name: Human Recombinant CHST15 protein, His Tag
SKU: V2LY-0526-LY3133
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CHST15 protein, His Tag consist of Amino Acid: 99-561 and predicts a molecular mass of 56 kDa.

Molecule Mass

56 kDa

Sequence

Amino Acid: 99-561

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>97% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Carbohydrate Sulfotransferase 15

Function

Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO4) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo.

Biological Process

Chondroitin sulfate biosynthetic process Source: Reactome
Hexose biosynthetic process Source: UniProtKB

Cellular Location

Golgi apparatus membrane. A small fraction may also be present at the cell surface, where it acts as a B-cell receptor.

Topology

Cytoplasmic: 1-80
Helical: 81-101
Lumenal: 102-561

PTM

Glycosylated.

Bhattacharyya, S., Feferman, L., Han, X., Xia, K., Zhang, F., Linhardt, R. J., & Tobacman, J. K. (2020). Increased CHST15 follows decline in arylsulfatase B (ARSB) and disinhibition of non-canonical WNT signaling: potential impact on epithelial and mesenchymal identity. Oncotarget, 11(24), 2327.

Matsuda, Y., Fujii, Y., Matsukawa, M., Ishiwata, T., Nishimura, M., & Arai, T. (2019). Overexpression of carbohydrate sulfotransferase 15 in pancreatic cancer stroma is associated with worse prognosis. Oncology letters, 18(4), 4100-4105.

Liu, L. C., Wang, Y. L., Lin, P. L., Zhang, X., Cheng, W. C., Liu, S. H., ... & Wang, S. C. (2019). Long noncoding RNA HOTAIR promotes invasion of breast cancer cells through chondroitin sulfotransferase CHST15. International journal of cancer, 145(9), 2478-2487.

Sato, H., Sagara, S., Nakajima, N., Akimoto, T., Suzuki, K., Yoneyama, H., ... & Yahagi, N. (2017). Prevention of esophageal stricture after endoscopic submucosal dissection using RNA-based silencing of carbohydrate sulfotransferase 15 in a porcine model. Endoscopy, 49(05), 491-497.

Kai, Y., Tomoda, K., Yoneyama, H., Kitabatake, M., Nakamura, A., Ito, T., ... & Kimura, H. (2017). Silencing of carbohydrate sulfotransferase 15 hinders murine pulmonary fibrosis development. Molecular Therapy-Nucleic Acids, 6, 163-172.

Ito, Z., Takakura, K., Suka, M., Kanai, T., Saito, R., Fujioka, S., ... & Ohkusa, T. (2017). Prognostic impact of carbohydrate sulfotransferase 15 in patients with pancreatic ductal adenocarcinoma. Oncology letters, 13(6), 4799-4805.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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