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Mouse Anti-CHST15 Recombinant Antibody (388421) (CBMAB-C5597-LY)

This product is antibody recognizes CHST15. The antibody 388421 immunoassay techniques such as: WB.
See all CHST15 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
388421
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Sulfotransferase 15/CHST15, Ser99-Thr561, Accession # Q7LFX5
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Carbohydrate Sulfotransferase 15
Introduction
Chondroitin sulfate (CS) is a glycosaminoglycan which is an important structural component of the extracellular matrix and which links to proteins to form proteoglycans. Chondroitin sulfate E (CS-E) is an isomer of chondroitin sulfate in which the C-4 and C-6 hydroxyl groups are sulfated. This gene encodes a type II transmembrane glycoprotein that acts as a sulfotransferase to transfer sulfate to the C-6 hydroxal group of chondroitin sulfate. This gene has also been identified as being co-expressed with RAG1 in B-cells and as potentially acting as a B-cell surface signaling receptor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]
Entrez Gene ID
51363
UniProt ID
Q7LFX5
Alternative Names
Carbohydrate Sulfotransferase 15; Carbohydrate (N-Acetylgalactosamine 4-Sulfate 6-O) Sulfotransferase 15; N-Acetylgalactosamine 4-Sulfate 6-O-Sulfotransferase; B-Cell RAG-Associated Gene Protein; GALNAC4S-6ST; HBRAG; BRAG;
Function
Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO4) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo.
Biological Process
Chondroitin sulfate biosynthetic process Source: Reactome
Hexose biosynthetic process Source: UniProtKB
Cellular Location
Golgi apparatus membrane. A small fraction may also be present at the cell surface, where it acts as a B-cell receptor.
Topology
Cytoplasmic: 1-80
Helical: 81-101
Lumenal: 102-561
PTM
Glycosylated.

Bhattacharyya, S., Feferman, L., Han, X., Xia, K., Zhang, F., Linhardt, R. J., & Tobacman, J. K. (2020). Increased CHST15 follows decline in arylsulfatase B (ARSB) and disinhibition of non-canonical WNT signaling: potential impact on epithelial and mesenchymal identity. Oncotarget, 11(24), 2327.

Matsuda, Y., Fujii, Y., Matsukawa, M., Ishiwata, T., Nishimura, M., & Arai, T. (2019). Overexpression of carbohydrate sulfotransferase 15 in pancreatic cancer stroma is associated with worse prognosis. Oncology letters, 18(4), 4100-4105.

Liu, L. C., Wang, Y. L., Lin, P. L., Zhang, X., Cheng, W. C., Liu, S. H., ... & Wang, S. C. (2019). Long noncoding RNA HOTAIR promotes invasion of breast cancer cells through chondroitin sulfotransferase CHST15. International journal of cancer, 145(9), 2478-2487.

Sato, H., Sagara, S., Nakajima, N., Akimoto, T., Suzuki, K., Yoneyama, H., ... & Yahagi, N. (2017). Prevention of esophageal stricture after endoscopic submucosal dissection using RNA-based silencing of carbohydrate sulfotransferase 15 in a porcine model. Endoscopy, 49(05), 491-497.

Kai, Y., Tomoda, K., Yoneyama, H., Kitabatake, M., Nakamura, A., Ito, T., ... & Kimura, H. (2017). Silencing of carbohydrate sulfotransferase 15 hinders murine pulmonary fibrosis development. Molecular Therapy-Nucleic Acids, 6, 163-172.

Ito, Z., Takakura, K., Suka, M., Kanai, T., Saito, R., Fujioka, S., ... & Ohkusa, T. (2017). Prognostic impact of carbohydrate sulfotransferase 15 in patients with pancreatic ductal adenocarcinoma. Oncology letters, 13(6), 4799-4805.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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