Human CHST15 ELISA Kit (V2LY-0626-LY3498)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.043 ng/mL
Detection Range
0.1-40 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
64.9 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Carbohydrate Sulfotransferase 15
Function
Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO4) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo.
Biological Process
Chondroitin sulfate biosynthetic process Source: Reactome
Hexose biosynthetic process Source: UniProtKB
Cellular Location
Golgi apparatus membrane. A small fraction may also be present at the cell surface, where it acts as a B-cell receptor.
Topology
Cytoplasmic: 1-80
Helical: 81-101
Lumenal: 102-561
PTM
Glycosylated.

Bhattacharyya, S., Feferman, L., Han, X., Xia, K., Zhang, F., Linhardt, R. J., & Tobacman, J. K. (2020). Increased CHST15 follows decline in arylsulfatase B (ARSB) and disinhibition of non-canonical WNT signaling: potential impact on epithelial and mesenchymal identity. Oncotarget, 11(24), 2327.

Matsuda, Y., Fujii, Y., Matsukawa, M., Ishiwata, T., Nishimura, M., & Arai, T. (2019). Overexpression of carbohydrate sulfotransferase 15 in pancreatic cancer stroma is associated with worse prognosis. Oncology letters, 18(4), 4100-4105.

Liu, L. C., Wang, Y. L., Lin, P. L., Zhang, X., Cheng, W. C., Liu, S. H., ... & Wang, S. C. (2019). Long noncoding RNA HOTAIR promotes invasion of breast cancer cells through chondroitin sulfotransferase CHST15. International journal of cancer, 145(9), 2478-2487.

Sato, H., Sagara, S., Nakajima, N., Akimoto, T., Suzuki, K., Yoneyama, H., ... & Yahagi, N. (2017). Prevention of esophageal stricture after endoscopic submucosal dissection using RNA-based silencing of carbohydrate sulfotransferase 15 in a porcine model. Endoscopy, 49(05), 491-497.

Kai, Y., Tomoda, K., Yoneyama, H., Kitabatake, M., Nakamura, A., Ito, T., ... & Kimura, H. (2017). Silencing of carbohydrate sulfotransferase 15 hinders murine pulmonary fibrosis development. Molecular Therapy-Nucleic Acids, 6, 163-172.

Ito, Z., Takakura, K., Suka, M., Kanai, T., Saito, R., Fujioka, S., ... & Ohkusa, T. (2017). Prognostic impact of carbohydrate sulfotransferase 15 in patients with pancreatic ductal adenocarcinoma. Oncology letters, 13(6), 4799-4805.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CHST15 protein, His Tag (CAT#: V2LY-0526-LY3133)

Related Products

Human Recombinant CHST15 protein, His Tag (CAT#: V2LY-0526-LY3133)

Mouse Anti-CHST15 Recombinant Antibody (C5597) (CAT#: CBMAB-C5597-LY)

Isotype control

For research use only. Not intended for any clinical use.

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