Human Recombinant CLDN4 (Nanodisc) protein, His & Strep Tag (V2LY-0526-LY3205)

Name: Human Recombinant CLDN4 (Nanodisc) protein, His & Strep Tag
SKU: V2LY-0526-LY3205
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His & Strep Tag

Protein Construction

This product is Human Recombinant CLDN4 (Nanodisc) protein, His & Strep Tag consist of Amino Acid: 1-209 and predicts a molecular mass of 24.62 kDa.

Molecule Mass

24.62 kDa

Sequence

Amino Acid: 1-209

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥85% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Tirs, NaCl, Glycerol, DTT

Preservative

None

Storage

Samples are stable for up to three months from date of receipt at -70°C. Store it under sterile conditions at -70°C or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Claudin 4

Function

Channel-forming tight junction protein that mediates paracellular chloride transport in the kidney. Plays a critical role in the paracellular reabsorption of filtered chloride in the kidney collecting ducts. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

Biological Process

Bicellular tight junction assembly Source: GO_Central
Calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
Cell adhesion Source: GO_Central
Chloride transport Source: UniProtKB-KW
Circadian rhythm Source: Ensembl
Establishment of skin barrier Source: UniProtKB
Female pregnancy Source: Ensembl
Positive regulation of cell migration Source: ARUK-UCL
Positive regulation of metallopeptidase activity Source: ARUK-UCL
Positive regulation of wound healing Source: ARUK-UCL
Regulation of cell morphogenesis Source: ARUK-UCL
Renal absorption Source: UniProtKB
Response to progesterone Source: Ensembl

Cellular Location

Cell membrane; Tight junction. CLDN4 is required for tight junction localization in the kidney.

Involvement in disease

CLDN4 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.

Topology

Cytoplasmic: 1-7
Helical: 8-28
Extracellular: 29-81
Helical: 82-102
Cytoplasmic: 103-117
Helical: 118-138
Extracellular: 139-160
Helical: 161-181
Cytoplasmic: 182-209

PTM

Phosphorylated. Phosphorylation by EPHA2 is stimulated by EFNA1 and alters interaction with TJP1.

Li, H., Neelankal John, A., Nagatake, T., Hamazaki, Y., & Jiang, F. X. (2020). Claudin 4 in pancreatic β cells is involved in regulating the functional state of adult islets. FEBS open bio, 10(1), 28-40.

Rambabu, M., & Jayanthi, S. (2020). Screening approaches against claudin-4 focusing on therapeutics through molecular docking and the analysis of their relative dynamics: a theoretical approach. Journal of Receptors and Signal Transduction, 40(5), 436-441.

Luo, Y., Kishi, S., Sasaki, T., Ohmori, H., Fujiwara‐Tani, R., Mori, S., ... & Kuniyasu, H. (2020). Targeting claudin‐4 enhances chemosensitivity in breast cancer. Cancer science, 111(5), 1840.

Nakashima, C., Yamamoto, K., Kishi, S., Sasaki, T., Ohmori, H., Fujiwara-Tani, R., ... & Kuniyasu, H. (2020). Clostridium perfringens enterotoxin induces claudin-4 to activate YAP in oral squamous cell carcinomas. Oncotarget, 11(4), 309.

Owari, T., Sasaki, T., Fujii, K., Fujiwara-Tani, R., Kishi, S., Mori, S., ... & Kuniyasu, H. (2020). Role of Nuclear Claudin-4 in Renal Cell Carcinoma. International Journal of Molecular Sciences, 21(21), 8340.

Ding, G., Liu, J., Shao, Q., Wang, B., Feng, J., Li, Y., ... & Xiao, Y. (2020). Porcine reproductive and respiratory syndrome virus structural protein GP3 regulates claudin 4 to facilitate the early stages of infection. Journal of Virology, 94(20), e00124-20.

Nishiguchi, Y., Fujiwara-Tani, R., Sasaki, T., Luo, Y., Ohmori, H., Kishi, S., ... & Kuniyasu, H. (2019). Targeting claudin-4 enhances CDDP-chemosensitivity in gastric cancer. Oncotarget, 10(22), 2189.

Rambabu, M., & Jayanthi, S. (2019). Virtual screening of National Cancer Institute database for claudin‐4 inhibitors: Synthesis, biological evaluation, and molecular dynamics studies. Journal of cellular biochemistry, 120(5), 8588-8600.

Sasaki, T., Fujiwara‐Tani, R., Kishi, S., Mori, S., Luo, Y., Ohmori, H., ... & Kuniyasu, H. (2019). Targeting claudin‐4 enhances chemosensitivity of pancreatic ductal carcinomas. Cancer medicine, 8(15), 6700-6708.

Fujiwara-Tani, R., Sasaki, T., Luo, Y. I., Goto, K., Kawahara, I., Nishiguchi, Y., ... & Kuniyasu, H. (2018). Anti-claudin-4 extracellular domain antibody enhances the antitumoral effects of chemotherapeutic and antibody drugs in colorectal cancer. Oncotarget, 9(100), 37367.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CLDN4 (Nanodisc) protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY3206)

Human Recombinant CLDN4, Detergent protein, Flag Tag (CAT#: V2LY-0526-LY3204)

Human Recombinant CLDN4-VLP protein, Flag Tag (CAT#: V2LY-0526-LY3207)

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Isotype control

For research use only. Not intended for any clinical use.

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