Human Recombinant COASY protein, His Tag (V2LY-0526-LY3308)

Name: Human Recombinant COASY protein, His Tag
SKU: V2LY-0526-LY3308
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant COASY protein, His Tag consist of Amino Acid: 296-564 and predicts a molecular mass of 32.5 kDa.

Molecule Mass

32.5 kDa

Sequence

Amino Acid: 296-564

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Coenzyme A Synthase

Function

Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.

Biological Process

Coenzyme A biosynthetic process Source: UniProtKB

Cellular Location

Mitochondrion matrix; Cytoplasm. The protein is mainly present in the mitochondrial matrix, probably anchored to the inner mitochondrial membrane, but is also present in cell lysate.

Involvement in disease

Neurodegeneration with brain iron accumulation 6 (NBIA6):
A neurodegenerative disorder associated with iron accumulation in the brain, primarily in the basal ganglia. It is characterized by progressive motor and cognitive dysfunction beginning in childhood or young adulthood. Patients show extrapyramidal motor signs, such as spasticity, dystonia, and parkinsonism.
Pontocerebellar hypoplasia 12 (PCH12):
A form of pontocerebellar hypoplasia, a disorder characterized by structural defects of the pons and cerebellum, evident upon brain imaging. PCH12 is an autosomal recessive form characterized by onset in utero and death in infancy. Brain imaging shows microcephaly, cerebellar hypoplasia, micrognathia, and multiple contractures.

Ferrandon, S., DeVecchio, J., Duraes, L., Chouhan, H., Karagkounis, G., Davenport, J., ... & Kalady, M. F. (2020). CoA Synthase (COASY) mediates radiation resistance via PI3K signaling in rectal cancer. Cancer research, 80(2), 334-346.

Chambers, E., Hermann, E., Davis, D., Montgomery, M., Lucas, E., & Chowanadisai, W. (2020). Brain MRI Phenotypes Associated with Polymorphisms at or near the MAPT (Microtubule Associated Protein Tau) and COASY (Coenzyme A Synthase) Genes. Current Developments in Nutrition, 4(Supplement_2), 1246-1246.

van Dijk, T., Ferdinandusse, S., Ruiter, J. P., Alders, M., Mathijssen, I. B., Parboosingh, J. S., ... & Baas, F. (2018). Biallelic loss of function variants in COASY cause prenatal onset pontocerebellar hypoplasia, microcephaly, and arthrogryposis. European Journal of Human Genetics, 26(12), 1752-1758.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

COASY Matched Antibody Pair (284) (CAT#: APMAB-284LY)

COASY Matched Antibody Pair (285) (CAT#: APMAB-285LY)

Antibody Pairs

COASY Matched Antibody Pair (284) (CAT#: APMAB-284LY)

COASY Matched Antibody Pair (285) (CAT#: APMAB-285LY)

Related Products

COASY Matched Antibody Pair (284) (CAT#: APMAB-284LY)

Rabbit Anti-COASY Recombinant Antibody (CBFYC-2026) (CAT#: V2LY-1206-LY564)

Mouse Anti-COASY Recombinant Antibody (CBFYC-2024) (CAT#: V2LY-1206-LY562)

Mouse Anti-COASY Recombinant Antibody (CBFYC-2025) (CAT#: V2LY-1206-LY563)

COASY Matched Antibody Pair (285) (CAT#: APMAB-285LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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