Human Recombinant CPB1 protein, His Tag (V2LY-0526-LY2574)

Name: Human Recombinant CPB1 protein, His Tag
SKU: V2LY-0526-LY2574
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CPB1 protein, His Tag consist of Amino Acid: 1-417 and predicts a molecular mass of 47 kDa.

Molecule Mass

47 kDa

Sequence

Amino Acid: 1-417

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Carboxypeptidase B1

Biological Process

Proteolysis Source: MGI

Cellular Location

Secreted; Zymogen granule lumen

Kothari, C., Clemenceau, A., Ouellette, G., Ennour-Idrissi, K., Michaud, A., Diorio, C., & Durocher, F. (2021). Is Carboxypeptidase B1 a Prognostic Marker for Ductal Carcinoma In Situ?. Cancers, 13(7), 1726.

Tamura, K., Yu, J., Hata, T., Suenaga, M., Shindo, K., Abe, T., ... & Goggins, M. (2018). Mutations in the pancreatic secretory enzymes CPA1 and CPB1 are associated with pancreatic cancer. Proceedings of the National Academy of Sciences, 115(18), 4767-4772.

Yu, S. L., Han, S., Kim, H. R., Park, J. W., Jin, D. I., & Kang, J. (2017). Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation. International Journal of Molecular Medicine, 40(5), 1397-1404.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat Cpb1 ELISA Kit (CAT#: V2LY-0626-LY2105)

Human CPB1 ELISA Kit (CAT#: V2LY-0626-LY5554)

Pig CPB1 ELISA Kit (CAT#: V2LY-0626-LY940)

CPB1 Matched Antibody Pair (294) (CAT#: APMAB-294LY)

CPB1 Matched Antibody Pair (295) (CAT#: APMAB-295LY)

Mouse Recombinant CPB1 protein, His Tag (CAT#: V2LY-0526-LY7916)

Antibody Pairs

CPB1 Matched Antibody Pair (294) (CAT#: APMAB-294LY)

CPB1 Matched Antibody Pair (295) (CAT#: APMAB-295LY)

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Rat Cpb1 ELISA Kit (CAT#: V2LY-0626-LY2105)

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Mouse Anti-CPB1 Recombinant Antibody (CBFYC-2147) (CAT#: V2LY-1206-LY928)

Mouse Anti-CPB1 Recombinant Antibody (CBFYC-2148) (CAT#: V2LY-1206-LY929)

Rabbit Anti-CPB1 Recombinant Antibody (329) (CAT#: V2LY-1206-LY925)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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