Human Recombinant CPE protein, hFc Tag (V2LY-0526-LY2576)

Name: Human Recombinant CPE protein, hFc Tag
SKU: V2LY-0526-LY2576
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant CPE protein, hFc Tag consist of Amino Acid: 1-453 and predicts a molecular mass of 74.6 kDa.

Molecule Mass

74.6 kDa

Sequence

Amino Acid: 1-453

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS, Trehalose, Mannitol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Carboxypeptidase E

Function

Sorting receptor that directs prohormones to the regulated secretory pathway. Acts also as a prohormone processing enzyme in neuro/endocrine cells, removing dibasic residues from the C-terminal end of peptide hormone precursors after initial endoprotease cleavage.

Biological Process

Cardiac left ventricle morphogenesis Source: BHF-UCL
Cellular protein modification process Source: UniProtKB
Neuropeptide signaling pathway Source: UniProtKB
Peptide metabolic process Source: GO_Central
Protein localization to membrane Source: BHF-UCL
Protein processing Source: GO_Central
Wnt signaling pathway Source: CACAO

Cellular Location

Isoform 1: Secreted; Secretory vesicle; Secretory vesicle membrane. Associated with the secretory granule membrane through direct binding to lipid rafts in intragranular conditions.

Li, N., Teng, S. W., Zhao, L., Li, J. R., Xu, J. L., Shuai, J. C., & Chen, Z. Y. (2021). Carboxypeptidase E regulates activity-dependent TrkB neuronal surface insertion and hippocampal memory. Journal of Neuroscience, 41(33), 6987-7002.

Moon, Y., Moon, R., Roh, H., Chang, S., Lee, S., & Park, H. (2020). HIF-1α-dependent induction of carboxypeptidase A4 and carboxypeptidase E in hypoxic human adipose-derived stem cells. Molecules and Cells, 43(11), 945.

Chougule, A., Kolli, V., Baroi, S., Ebraheim, N., Czernik, P. J., Loh, Y. P., & Lecka‐Czernik, B. (2020). Nonenzymatic and trophic activities of carboxypeptidase e regulate bone mass and bioenergetics of skeletal stem cells in mice. JBMR plus, 4(9), e10392.

Liang, C., Carrel, D., Omelchenko, A., Kim, H., Patel, A., Fanget, I., & Firestein, B. L. (2019). Cortical neuron migration and dendrite morphology are regulated by carboxypeptidase E. Cerebral Cortex, 29(7), 2890-2903.

Fan, S., Gao, X., Chen, P., & Li, X. (2019). Carboxypeptidase E-ΔN promotes migration, invasiveness, and epithelial–mesenchymal transition of human osteosarcoma cells via the Wnt–β-catenin pathway. Biochemistry and Cell Biology, 97(4), 446-453.

Yang, X., Lou, H., Chen, Y. T., Huang, S. F., & Loh, Y. P. (2019). A novel 40kDa N-terminal truncated Carboxypeptidase E splice variant: Cloning, cDNA sequence analysis and role in regulation of metastatic genes in human cancers. Genes & cancer, 10(5-6), 160.

Xiao, L., Yang, X., & Loh, Y. P. (2019). Neurotrophic, gene regulation, and cognitive functions of carboxypeptidase E-neurotrophic factor-α1 and its variants. Frontiers in Neuroscience, 13, 243.

Fricker, L. D. (2018). Carboxypeptidase E and the identification of novel neuropeptides as potential therapeutic targets. Advances in pharmacology, 82, 85-102.

Ji, L., Wu, H. T., Qin, X. Y., & Lan, R. (2017). Dissecting carboxypeptidase E: properties, functions and pathophysiological roles in disease. Endocrine Connections, 6(4), R18-R38.

Cong, L., Cheng, Y., Cawley, N. X., Murthy, S. R., & Loh, Y. P. (2017). A novel single nucleotide T980C polymorphism in the human carboxypeptidase E gene results in loss of neuroprotective function. PLoS One, 12(1), e0170169.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Cpe ELISA Kit (CAT#: V2LY-0626-LY2121)

Human CPE ELISA Kit (CAT#: V2LY-0626-LY5555)

Human Recombinant CPE protein, His Tag (CAT#: V2LY-0526-LY2577)

Related Products

Human CPE ELISA Kit (CAT#: V2LY-0626-LY5555)

Mouse Cpe ELISA Kit (CAT#: V2LY-0626-LY2121)

Human Recombinant CPE protein, His Tag (CAT#: V2LY-0526-LY2577)

Mouse Anti-CPE Recombinant Antibody (CBXC-1179) (CAT#: V2LY-1206-LY944)

Mouse Anti-CPE Recombinant Antibody (C6461) (CAT#: V2LY-1206-LY941)

Mouse Anti-CPE Recombinant Antibody (CBYY-C0036) (CAT#: V2LY-1206-LY943)

Rabbit Anti-CPE Recombinant Antibody (CBFYC-2156) (CAT#: CBMAB-C2224-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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