Human Recombinant CPLX1 protein, His Tag (V2LY-0526-LY3348)

Name: Human Recombinant CPLX1 protein, His Tag
SKU: V2LY-0526-LY3348
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CPLX1 protein, His Tag consist of Amino Acid: 1-134 and predicts a molecular mass of 15.99 kDa.

Molecule Mass

15.99 kDa

Verified

HPLC

Sequence

Amino Acid: 1-134

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC. ≥90% as determined by SEC-MALS(Routinely tested).

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Complexin 1

Function

Positively regulates a late step in exocytosis of various cytoplasmic vesicles, such as synaptic vesicles and other secretory vesicles (PubMed:21785414).

Organizes the SNAREs into a cross-linked zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion, thereby preventing SNAREs from releasing neurotransmitters until an action potential arrives at the synapse (PubMed:21785414).

Also involved in glucose-induced secretion of insulin by pancreatic beta-cells. Essential for motor behavior.

Biological Process

Chemical synaptic transmission Source: ProtInc
Exocytic insertion of neurotransmitter receptor to postsynaptic membrane Source: Ensembl
Exocytosis Source: ProtInc
Insulin secretion Source: Ensembl
Neurotransmitter secretion Source: Reactome
Neurotransmitter transport Source: Reactome
Regulation of exocytosis Source: ParkinsonsUK-UCL
Regulation of neurotransmitter secretion Source: GO_Central
Regulation of synaptic vesicle fusion to presynaptic active zone membrane Source: Ensembl
Synaptic vesicle exocytosis Source: GO_Central

Cellular Location

Cytosol; Perikaryon; Presynapse. Enriched at synaptic-releasing sites in mature neurons.

Involvement in disease

Developmental and epileptic encephalopathy 63 (DEE63):
A form of epileptic encephalopathy, a heterogeneous group of severe childhood onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. DEE63 is an autosomal recessive disease with onset in infancy.

Lux, U. T., Ehrenberg, J., Joachimsthaler, A., Atorf, J., Pircher, B., Reim, K., ... & Brandstätter, J. H. (2021). Cell Types and Synapses Expressing the SNARE Complex Regulating Proteins Complexin 1 and Complexin 2 in Mammalian Retina. International Journal of Molecular Sciences, 22(15), 8131.

Xu, Y., Zhao, X. M., Liu, J., Wang, Y. Y., Xiong, L. L., He, X. Y., & Wang, T. H. (2020). Complexin I knockout rats exhibit a complex neurobehavioral phenotype including profound ataxia and marked deficits in lifespan. Pflügers Archiv-European Journal of Physiology, 472(1), 117-133.

Lahut, S., Gispert, S., Ömür, Ö., Depboylu, C., Seidel, K., Domínguez-Bautista, J. A., ... & Auburger, G. (2017). Blood RNA biomarkers in prodromal PARK4 and rapid eye movement sleep behavior disorder show role of complexin 1 loss for risk of Parkinson's disease. Disease models & mechanisms, 10(5), 619-631.

Redler, S., Strom, T. M., Wieland, T., Cremer, K., Engels, H., Distelmaier, F., ... & Wieczorek, D. (2017). Variants in CPLX1 in two families with autosomal-recessive severe infantile myoclonic epilepsy and ID. European Journal of Human Genetics, 25(7), 889-893.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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