Human Recombinant CPNE1 protein, SUMO Tag (V2LY-0526-LY3354)

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Basic Information

Expressed Host
Baculovirus-Insect Cells
Protein Species
Human
Tag
SUMO Tag
Protein Construction
This product is Human Recombinant CPNE1 protein, SUMO Tag consist of Amino Acid: 1-537 and predicts a molecular mass of 72.7 kDa.
Molecule Mass
72.7 kDa
Sequence
Amino Acid: 1-537
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>95% as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile PBS
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
Copine 1
Function
Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes (PubMed:14674885).

Involved in the TNF-alpha receptor signaling pathway in a calcium-dependent manner (PubMed:14674885).

Exhibits calcium-dependent phospholipid binding properties (PubMed:9430674, PubMed:19539605).

Plays a role in neuronal progenitor cell differentiation; induces neurite outgrowth via a AKT-dependent signaling cascade and calcium-independent manner (PubMed:23263657, PubMed:25450385).

May recruit target proteins to the cell membrane in a calcium-dependent manner (PubMed:12522145).

May function in membrane trafficking (PubMed:9430674).

Involved in TNF-alpha-induced NF-kappa-B transcriptional repression by inducing endoprotease processing of the transcription factor NF-kappa-B p65/RELA subunit (PubMed:18212740).

Also induces endoprotease processing of NF-kappa-B p50/NFKB1, p52/NFKB2, RELB and REL (PubMed:18212740).
Biological Process
Cellular response to calcium ion Source: UniProtKB
Glycerophospholipid biosynthetic process Source: Reactome
Lipid metabolic process Source: ProtInc
Negative regulation of DNA binding Source: UniProtKB
Negative regulation of gene expression Source: UniProtKB
Negative regulation of NIK/NF-kappaB signaling Source: UniProtKB
Neuron projection extension Source: UniProtKB
Neutrophil degranulation Source: Reactome
Positive regulation of neuron differentiation Source: UniProtKB
Positive regulation of protein kinase B signaling Source: UniProtKB
Positive regulation of tumor necrosis factor-mediated signaling pathway Source: UniProtKB
Proteolysis Source: UniProtKB
Regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Vesicle-mediated transport Source: ProtInc
Cellular Location
Nucleus; Cell membrane; Cytoplasm. Translocates to the cell membrane in a calcium-dependent manner (PubMed:21087455, PubMed:25450385).

Wang, Y., Pan, S., He, X., Wang, Y., Huang, H., Chen, J., ... & Qin, X. (2021). CPNE1 enhances colorectal cancer cell growth, glycolysis, and drug resistance through regulating the AKT-GLUT1/HK2 Pathway. OncoTargets and therapy, 14, 699.

Du, W., Liu, T., Zhang, Y., Zeng, Y., Zhu, J., Tang, H., ... & Huang, J. A. (2020). MiR-195-5p is a potential factor responsible for CPNE1 differential expression between subtypes of non-small cell lung cancer. Journal of Cancer, 11(9), 2610.

Shao, Z., Ma, X., Zhang, Y., Sun, Y., Lv, W., He, K., ... & Gao, X. (2020). CPNE1 predicts poor prognosis and promotes tumorigenesis and radioresistance via the AKT singling pathway in triple‐negative breast cancer. Molecular carcinogenesis, 59(5), 533-544.

Choi, H. Y., Park, N., Lee, B., Choe, Y. I., Woo, D. K., Park, J. Y., & Yoo, J. C. (2020). CPNE1-mediated neuronal differentiation can be inhibited by HAX1 expression in HiB5 cells. Biochemical and biophysical research communications, 533(3), 319-324.

Kim, T. H., Sung, S. E., Yoo, J. C., Park, J. Y., Yi, G. S., Heo, J. Y., ... & Lee, D. Y. (2018). Copine1 regulates neural stem cell functions during brain development. Biochemical and biophysical research communications, 495(1), 168-173.

Liu, S., Tang, H., Zhu, J., Ding, H., Zeng, Y., Du, W., ... & Huang, J. A. (2018). High expression of Copine 1 promotes cell growth and metastasis in human lung adenocarcinoma. International journal of oncology, 53(6), 2369-2378.

Tang, H., Zhu, J., Du, W., Liu, S., Zeng, Y., Ding, Z., ... & Huang, J. (2018). CPNE1 is a target of miR-335-5p and plays an important role in the pathogenesis of non-small cell lung cancer. Journal of Experimental & Clinical Cancer Research, 37(1), 1-15.

Jiang, Z., Jiang, J., Zhao, B., Yang, H., Wang, Y., Guo, S., ... & Wang, J. (2018). CPNE1 silencing inhibits the proliferation, invasion and migration of human osteosarcoma cells. Oncology reports, 39(2), 643-650.

Yoo, J. C., Park, N., Lee, B., Nashed, A., Lee, Y. S., Kim, T. H., ... & Park, J. Y. (2017). 14-3-3γ regulates Copine1-mediated neuronal differentiation in HiB5 hippocampal progenitor cells. Experimental cell research, 356(1), 85-92.

Liang, J., Zhang, J., Ruan, J., Mi, Y., Hu, Q., Wang, Z., & Wei, B. (2017). CPNE1 is a useful prognostic marker and is associated with TNF receptor-associated factor 2 (TRAF2) expression in prostate cancer. Medical science monitor: international medical journal of experimental and clinical research, 23, 5504.

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For research use only. Not intended for any clinical use.

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