Human Recombinant CSTB protein, His Tag (V2LY-0526-LY3474)

Name: Human Recombinant CSTB protein, His Tag
SKU: V2LY-0526-LY3474
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CSTB protein, His Tag consist of Amino Acid: 2-98 and predicts a molecular mass of 12.5 kDa.

Molecule Mass

12.5 kDa

Sequence

Amino Acid: 2-98

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Cystatin B

Function

This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.

Biological Process

Adult locomotory behavior Source: Ensembl
Negative regulation of peptidase activity Source: BHF-UCL
Negative regulation of proteolysis Source: UniProtKB
Neutrophil degranulation Source: Reactome

Cellular Location

Cytoplasm; Nucleus

Involvement in disease

Epilepsy, progressive myoclonic 1 (EPM1):
A form of progressive myoclonic epilepsy, a clinically and genetically heterogeneous group of disorders defined by the combination of action and reflex myoclonus, other types of epileptic seizures, and progressive neurodegeneration and neurocognitive impairment. EPM1 is an autosomal recessive form characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The onset, occurring between 6 and 13 years of age, is characterized by convulsions. Myoclonus begins 1 to 5 years later. The twitchings occur predominantly in the proximal muscles of the extremities and are bilaterally symmetrical, although asynchronous. At first small, they become late in the clinical course so violent that the victim is thrown to the floor. Mental deterioration and eventually dementia develop.

Daura, E., Tegelberg, S., Yoshihara, M., Jackson, C., Simonetti, F., Aksentjeff, K., ... & Joensuu, T. (2021). Cystatin B-deficiency triggers ectopic histone H3 tail cleavage during neurogenesis. Neurobiology of Disease, 156, 105418.

Wu, Y., Whittaker, H. T., Noy, S., Cleverley, K., Brault, V., Herault, Y., ... & Wiseman, F. K. (2021). The effects of Cstb duplication on APP/amyloid-β pathology and cathepsin B activity in a mouse model. Plos one, 16(7), e0242236.

Di Matteo, F., Pipicelli, F., Kyrousi, C., Tovecci, I., Penna, E., Crispino, M., ... & Cappello, S. (2020). Cystatin B is essential for proliferation and interneuron migration in individuals with EPM 1 epilepsy. EMBO molecular medicine, 12(6), e11419.

Guan, W., Wang, X., Lin, Q., Zhang, J., Ren, W., & Xu, G. (2019). Transforming growth factor‑β/miR‑143‑3p/cystatin B axis is a therapeutic target in human ovarian cancer. International journal of oncology, 55(1), 267-276.

Penna, E., Cerciello, A., Chambery, A., Russo, R., Cernilogar, F. M., Pedone, E. M., ... & Crispino, M. (2019). Cystatin B involvement in synapse physiology of rodent brains and human cerebral organoids. Frontiers in Molecular Neuroscience, 195.

Unić, A., Derek, L., Duvnjak, M., Patrlj, L., Rakić, M., Kujundžić, M., ... & Grgurević, I. (2018). Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis. Annals of Clinical Biochemistry, 55(3), 355-362.

O'Brien, A., Marshall, C. R., Blaser, S., Ray, P. N., & Yoon, G. (2017). Severe neurodegeneration, progressive cerebral volume loss and diffuse hypomyelination associated with a homozygous frameshift mutation in CSTB. European Journal of Human Genetics, 25(6), 775-778.

Ma, Y., Chen, Y., & Petersen, I. (2017). Expression and epigenetic regulation of cystatin B in lung cancer and colorectal cancer. Pathology-Research and Practice, 213(12), 1568-1574.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

CSTB Matched Antibody Pair (306) (CAT#: APMAB-306LY)

CSTB Matched Antibody Pair (307) (CAT#: APMAB-307LY)

Antibody Pairs

CSTB Matched Antibody Pair (306) (CAT#: APMAB-306LY)

CSTB Matched Antibody Pair (307) (CAT#: APMAB-307LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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