Human Recombinant CXADR protein, His & hFc Tag (V2LY-0526-LY3428)

Name: Human Recombinant CXADR protein, His & hFc Tag
SKU: V2LY-0526-LY3428
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His & hFc Tag

Protein Construction

This product is Human Recombinant CXADR protein, His & hFc Tag consist of Amino Acid: 1-237 and predicts a molecular mass of 52 kDa.

Molecule Mass

52 kDa

Sequence

Amino Acid: 1-237

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>92% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CXADR, Ig-Like Cell Adhesion Molecule

Function

Component of the epithelial apical junction complex that may function as a homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair.

(Microbial infection) Acts as a receptor for adenovirus type C.

(Microbial infection) Acts as a receptor for Coxsackievirus B1 to B6.

Biological Process

Actin cytoskeleton reorganization Source: UniProtKB
AV node cell-bundle of His cell adhesion involved in cell communication Source: BHF-UCL
AV node cell to bundle of His cell communication Source: UniProtKB
Cardiac muscle fiber development Source: UniProtKB
Cell-cell junction organization Source: UniProtKB
Defense response to virus Source: UniProtKB
Epithelial structure maintenance Source: UniProtKB
Gamma-delta T cell activation Source: UniProtKB
Germ cell migration Source: UniProtKB
Heart development Source: UniProtKB
Heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
Homotypic cell-cell adhesion Source: UniProtKB
Leukocyte migration Source: Reactome
Mitochondrion organization Source: UniProtKB
Neutrophil chemotaxis Source: UniProtKB
Regulation of AV node cell action potential Source: BHF-UCL
Regulation of immune response Source: Reactome
Transepithelial transport Source: UniProtKB

Cellular Location

Isoform 1: Cell membrane; Basolateral cell membrane; Tight junction; Adherens junction. In epithelial cells localizes to the apical junction complex composed of tight and adherens junctions (PubMed:12297051). In airway epithelial cells localized to basolateral membrane but not to apical surface (PubMed:11316797).
Isoform 3&4&5: Secreted

Topology

Extracellular: 20-237
Helical: 238-258
Cytoplasmic: 259-365

PTM

N-glycosylated.
Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.

Zhang, Y., & Lui, W. Y. (2021). Coxsackievirus and Adenovirus Receptor (CXADR): Recent Findings and Its Role and Regulation in Spermatogenesis. Molecular Mechanisms in Spermatogenesis, 95-109.

Han, X., Zhao, Z. A., Yan, S., Lei, W., Wu, H., Lu, X. A., ... & Hu, S. (2020). CXADR‐like membrane protein protects against heart injury by preventing excessive pyroptosis after myocardial infarction. Journal of cellular and molecular medicine, 24(23), 13775-13788.

Nilchian, A., Johansson, J., Ghalali, A., Asanin, S. T., Santiago, A., Rosencrantz, O., ... & Fuxe, J. (2019). CXADR-mediated formation of an AKT inhibitory signalosome at tight junctions controls epithelial–mesenchymal plasticity in breast cancer. Cancer Research, 79(1), 47-60.

Kobayashi, T., Matsugo, H., Maruyama, J., Kamiki, H., Takada, A., Maeda, K., ... & Horimoto, T. (2019). Characterization of a novel species of adenovirus from Japanese microbat and role of CXADR as its entry factor. Scientific reports, 9(1), 1-13.

Jeong, Y., Ock, S. A., Yoo, J. G., Yu, D. Y., & Choi, I. (2019). The Cxadr–Adam10 complex plays pivotal roles in tight junction integrity and early trophoblast development in mice. Molecular Reproduction and Development, 86(11), 1628-1638.

Outhwaite, J. E., Patel, J., & Simmons, D. G. (2019). Secondary placental defects in Cxadr mutant mice. Frontiers in physiology, 622.

Kawada, M., Sakamoto, S., Inoue, H., Kaneko, M., Ogasawara, S., Kajikawa, M., ... & Kato, Y. (2019). Abstract B097: Development of a mouse-human chimeric antibody against human CXADR having a potent antitumor activity in animal models.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CXADR protein, His Tag (CAT#: V2LY-0526-LY3429)

Mouse Recombinant CXADR protein, His & hFc Tag (CAT#: V2LY-0526-LY8174)

Mouse Recombinant CXADR protein, His Tag (CAT#: V2LY-0526-LY8175)

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Isotype control

For research use only. Not intended for any clinical use.

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