Human Recombinant DBI protein, His Tag (V2LY-0526-LY3491)

Name: Human Recombinant DBI protein, His Tag
SKU: V2LY-0526-LY3491
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant DBI protein, His Tag consist of Amino Acid: 1-87 and predicts a molecular mass of 14 kDa.

Molecule Mass

14 kDa

Sequence

Amino Acid: 1-87

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Diazepam Binding Inhibitor, Acyl-CoA Binding Protein

Function

Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.

Biological Process

Acyl-CoA metabolic process Source: Reactome
Negative regulation of protein lipidation Source: UniProtKB
Phosphatidylcholine acyl-chain remodeling Source: UniProtKB
Positive regulation of CoA-transferase activity Source: UniProtKB
Positive regulation of phospholipid transport Source: UniProtKB

Cellular Location

Golgi apparatus; Endoplasmic reticulum. Golgi localization is dependent on ligand binding (PubMed:17953517).

Joseph, A., Chen, H., Anagnostopoulos, G., Montégut, L., Lafarge, A., Motiño, O., ... & Kroemer, G. (2021). Effects of acyl-coenzyme A binding protein (ACBP)/diazepam-binding inhibitor (DBI) on body mass index. Cell death & disease, 12(6), 1-14.

Thomas, A. M., Asnicar, F., Kroemer, G., & Segata, N. (2021). Genes Encoding Microbial Acyl Coenzyme A Binding Protein/Diazepam-Binding Inhibitor Orthologs Are Rare in the Human Gut Microbiome and Show No Links to Obesity. Applied and environmental microbiology, 87(12), e00471-21.

Mamoor, S. (2021). Over-expression of diazepam binding inhibitor, acyl-CoA binding protein in human endometrial cancer.

Alquier, T., Christian-Hinman, C. A., Alfonso, J., & Færgeman, N. J. (2021). From benzodiazepines to fatty acids and beyond: revisiting the role of ACBP/DBI. Trends in Endocrinology & Metabolism, 32(11), 890-903.

Joseph, A., Moriceau, S., Sica, V., Anagnostopoulos, G., Pol, J., Martins, I., ... & Kroemer, G. (2020). Metabolic and psychiatric effects of acyl coenzyme A binding protein (ACBP)/diazepam binding inhibitor (DBI). Cell death & disease, 11(7), 1-10.

Charmpilas, N., Ruckenstuhl, C., Sica, V., Büttner, S., Habernig, L., Dichtinger, S., ... & Kroemer, G. (2020). Acyl-CoA-binding protein (ACBP): a phylogenetically conserved appetite stimulator. Cell death & disease, 11(1), 1-10.

Bravo-San Pedro, J. M., Sica, V., Martins, I., Anagnostopoulos, G., Maiuri, C., & Kroemer, G. (2019). Cell-autonomous, paracrine and neuroendocrine feedback regulation of autophagy by DBI/ACBP (diazepam binding inhibitor, acyl-CoA binding protein): the obesity factor. Autophagy, 15(11), 2036-2038.

Bravo-San Pedro, J. M., Sica, V., Madeo, F., & Kroemer, G. (2019). Acyl-CoA-binding protein (ACBP): the elusive ‘hunger factor'linking autophagy to food intake. Cell Stress, 3(10), 312.

Bravo-San Pedro, J. M., Sica, V., Martins, I., Pol, J., Loos, F., Maiuri, M. C., ... & Kroemer, G. (2019). Acyl-CoA-binding protein is a lipogenic factor that triggers food intake and obesity. Cell metabolism, 30(4), 754-767.

Duman, C., Yaqubi, K., Hoffmann, A., Acikgöz, A. A., Korshunov, A., Bendszus, M., ... & Alfonso, J. (2019). Acyl-CoA-binding protein drives glioblastoma tumorigenesis by sustaining fatty acid oxidation. Cell metabolism, 30(2), 274-289.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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