Human Recombinant DCD protein, hFc Tag (V2LY-0526-LY3549)

Name: Human Recombinant DCD protein, hFc Tag
SKU: V2LY-0526-LY3549
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant DCD protein, hFc Tag consist of Amino Acid: 63-109 and predicts a molecular mass of 33.1 kDa.

Molecule Mass

33.1 kDa

Sequence

Amino Acid: 63-109

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Dermcidin

Function

DCD-1 displays antimicrobial activity thereby limiting skin infection by potential pathogens in the first few hours after bacterial colonization. Highly effective against E.coli, E.faecalis, S.aureus and C.albicans. Optimal pH and salt concentration resemble the conditions in sweat. Also exhibits proteolytic activity, cleaving on the C-terminal side of Arg and, to a lesser extent, Lys residues (PubMed:17448443).

Survival-promoting peptide promotes survival of neurons and displays phosphatase activity. It may bind IgG.

Biological Process

Antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
Antimicrobial humoral response Source: Reactome
Defense response to bacterium Source: UniProtKB-KW
Defense response to fungus Source: UniProtKB
Defense response to Gram-negative bacterium Source: UniProtKB
Defense response to Gram-positive bacterium Source: UniProtKB
Killing by host of symbiont cells Source: CACAO
Killing of cells of other organism Source: UniProtKB

Cellular Location

Secreted

Farshadzadeh, Z., Pourhajibagher, M., Taheri, B., Ekrami, A., Modarressi, M. H., Azimzadeh, M., & Bahador, A. (2022). Antimicrobial and anti-biofilm potencies of dermcidin-derived peptide DCD-1L against Acinetobacter baumannii: an in vivo wound healing model. BMC microbiology, 22(1), 1-13.

Qiu, F., Long, H., Zhang, L., Liu, J., Yang, Z., & Huang, X. (2022). Dermcidin Enhances the Migration, Invasion, and Metastasis of Hepatocellular Carcinoma Cells In Vitro and In Vivo. Journal of Clinical and Translational Hepatology, (000), 0-0.

Lager, T. W., Conner, C., Keating, C. R., Warshaw, J. N., & Panopoulos, A. D. (2021). Cell surface Grp78 and dermcidin cooperate to regulate breast cancer cell migration through Wnt signaling. Oncogene, 40(23), 4050-4059.

Alatas, E. T., Kara Polat, A., Kalayci, M., Dogan, G., & Akin Belli, A. (2019). Plasma dermcidin levels in acne patients, and the effect of isotretinoin treatment on dermcidin levels. Dermatologic Therapy, 32(5), e13044.

Song, C., de Groot, B. L., & Sansom, M. S. (2019). Lipid bilayer composition influences the activity of the antimicrobial peptide dermcidin channel. Biophysical journal, 116(9), 1658-1666.

Qiu, F., Qiu, F., Liu, L., Liu, J., Xu, J., & Huang, X. (2018). The Role of dermcidin in the diagnosis and staging of hepatocellular carcinoma. Genetic Testing and Molecular Biomarkers, 22(4), 218-223.

Mohanraj, U., Kinnunen, O., Kaya, M. E., Aranko, A. S., Viskari, H., & Linder, M. (2018). SUMO-based expression and purification of dermcidin-derived DCD-1L, a human antimicrobial peptide, in Escherichia coli. BioRxiv, 343418.

Nguyen, V. S., Tan, K. W., Ramesh, K., Chew, F. T., & Mok, Y. K. (2017). Structural basis for the bacterial membrane insertion of dermcidin peptide, DCD-1L. Scientific reports, 7(1), 1-11.

Zeth, K., & Sancho-Vaello, E. (2017). The human antimicrobial peptides dermcidin and LL-37 show novel distinct pathways in membrane interactions. Frontiers in chemistry, 5, 86.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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