Human Recombinant DDR1 protein, Biotin Conjugated, His & AVI Tag (V2LY-0526-LY3521)

Name: Human Recombinant DDR1 protein, Biotin Conjugated, His & AVI Tag
SKU: V2LY-0526-LY3521
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His & AVI Tag

Protein Construction

This product is Human Recombinant DDR1 protein, Biotin Conjugated, His & AVI Tag consist of Amino Acid: 1-416 and predicts a molecular mass of 47.22 kDa.

Molecule Mass

47.22 kDa

Verified

HPLC

Conjugates

Biotin

Sequence

Amino Acid: 1-416

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Discoidin Domain Receptor Tyrosine Kinase 1

Function

Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity).

Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.

Biological Process

Axon development Source: Ensembl
Branching involved in mammary gland duct morphogenesis Source: Ensembl
Cell adhesion Source: ProtInc
Collagen-activated tyrosine kinase receptor signaling pathway Source: UniProtKB
Ear development Source: Ensembl
Embryo implantation Source: Ensembl
Extracellular matrix organization Source: Reactome
Lactation Source: UniProtKB-KW
Mammary gland alveolus development Source: Ensembl
Multicellular organism development Source: GO_Central
Negative regulation of cell population proliferation Source: Ensembl
Neuron projection extension Source: Ensembl
Peptidyl-tyrosine autophosphorylation Source: UniProtKB
Positive regulation of kinase activity Source: GO_Central
Protein autophosphorylation Source: UniProtKB
Regulation of cell growth Source: Ensembl
Regulation of cell-matrix adhesion Source: Ensembl
Regulation of extracellular matrix disassembly Source: UniProtKB
Smooth muscle cell-matrix adhesion Source: UniProtKB
Smooth muscle cell migration Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Wound healing, spreading of cells Source: UniProtKB

Cellular Location

Isoform 1&2&4: Cell membrane
Isoform 3: Secreted

Topology

Extracellular: 21-417
Helical: 418-438
Cytoplasmic: 439-913

PTM

Autophosphorylated in response to fibrillar collagen binding.
Glycosylation of Asn-211, but apparently not of Asn-260 or Asn-394, prevents autophosphorylation from occurring in the absence of collagen.

Zhang, X., Hu, Y., Pan, Y., Xiong, Y., Zhang, Y., Han, M., ... & Zhang, B. (2022). DDR1 promotes hepatocellular carcinoma metastasis through recruiting PSD4 to ARF6. Oncogene, 41(12), 1821-1834.

Chen, L. Y., Zhi, Z., Wang, L., Zhao, Y. Y., Deng, M., Liu, Y. H., ... & Li, J. M. (2019). NSD2 circular RNA promotes metastasis of colorectal cancer by targeting miR‐199b‐5p‐mediated DDR1 and JAG1 signalling. The Journal of Pathology, 248(1), 103-115.

Zhavoronkov, A., Ivanenkov, Y. A., Aliper, A., Veselov, M. S., Aladinskiy, V. A., Aladinskaya, A. V., ... & Aspuru-Guzik, A. (2019). Deep learning enables rapid identification of potent DDR1 kinase inhibitors. Nature biotechnology, 37(9), 1038-1040.

Vehlow, A., & Cordes, N. (2019). DDR1 (discoidin domain receptor tyrosine kinase 1) drives glioblastoma therapy resistance by modulating autophagy. Autophagy, 15(8), 1487-1488.

Moll, S., Desmoulière, A., Moeller, M. J., Pache, J. C., Badi, L., Arcadu, F., ... & Prunotto, M. (2019). DDR1 role in fibrosis and its pharmacological targeting. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1866(11), 118474.

Yeh, Y. C., Lin, H. H., & Tang, M. J. (2019). Dichotomy of the function of DDR1 in cells and disease progression. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1866(11), 118473.

Belfiore, A., Malaguarnera, R., Nicolosi, M. L., Lappano, R., Ragusa, M., Morrione, A., & Vella, V. (2018). A novel functional crosstalk between DDR1 and the IGF axis and its relevance for breast cancer. Cell adhesion & migration, 12(4), 305-314.

Richter, H., Satz, A. L., Bedoucha, M., Buettelmann, B., Petersen, A. C., Harmeier, A., ... & Prunotto, M. (2018). DNA-encoded library-derived DDR1 inhibitor prevents fibrosis and renal function loss in a genetic mouse model of Alport syndrome. ACS chemical biology, 14(1), 37-49.

Takai, K., Drain, A. P., Lawson, D. A., Littlepage, L. E., Karpuj, M., Kessenbrock, K., ... & Werb, Z. (2018). Discoidin domain receptor 1 (DDR1) ablation promotes tissue fibrosis and hypoxia to induce aggressive basal-like breast cancers. Genes & development, 32(3-4), 244-257.

Deng, Y., Zhao, F., Hui, L., Li, X., Zhang, D., Lin, W., ... & Ning, Y. (2017). Suppressing miR-199a-3p by promoter methylation contributes to tumor aggressiveness and cisplatin resistance of ovarian cancer through promoting DDR1 expression. Journal of ovarian research, 10(1), 1-11.

Juskaite, V., Corcoran, D. S., & Leitinger, B. (2017). Collagen induces activation of DDR1 through lateral dimer association and phosphorylation between dimers. Elife, 6, e25716.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant DDR1 protein, GST Tag (CAT#: V2LY-0526-LY3519)

Human Recombinant DDR1, Active protein, GST Tag (CAT#: V2LY-0526-LY3520)

Human Recombinant DDR1 protein, His & AVI Tag (CAT#: V2LY-0526-LY3522)

Human Recombinant DDR1 protein, His Tag (CAT#: V2LY-0526-LY3523)

Human Recombinant DDR1 protein, His Tag-1 (CAT#: V2LY-0526-LY3524)

Human Recombinant DDR1 (S496A), Active protein, GST Tag (CAT#: V2LY-0526-LY3525)

Mouse Recombinant DDR1 protein, hFc Tag (CAT#: V2LY-0526-LY8187)

Mouse Recombinant DDR1 protein, GST Tag (CAT#: V2LY-0526-LY8188)

Mouse Recombinant DDR1 protein, His Tag (CAT#: V2LY-0526-LY8189)

Rat Recombinant DDR1 protein, hFc Tag (CAT#: V2LY-0526-LY9322)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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