Human Recombinant DKK1 protein, His Tag (V2LY-0526-LY3583)

Name: Human Recombinant DKK1 protein, His Tag
SKU: V2LY-0526-LY3583
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant DKK1 protein, His Tag consist of Amino Acid: 2-266 and predicts a molecular mass of 27.22 kDa.

Molecule Mass

27.22 kDa

Verified

HPLC

Sequence

Amino Acid: 2-266

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Dickkopf WNT Signaling Pathway Inhibitor 1

Function

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:22000856).

DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PubMed:17143291).

Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity).

Biological Process

Learning or memory Source: ARUK-UCL
Limb development Source: UniProtKB
Modulation of age-related behavioral decline Source: ARUK-UCL
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of canonical Wnt signaling pathway Source: ParkinsonsUK-UCL
Negative regulation of canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment Source: BHF-UCL
Negative regulation of cardiac muscle cell differentiation Source: BHF-UCL
Negative regulation of mesodermal cell fate specification Source: BHF-UCL
Negative regulation of ossification Source: UniProtKB
Negative regulation of pathway-restricted SMAD protein phosphorylation Source: BHF-UCL
Negative regulation of peptidyl-serine phosphorylation Source: BHF-UCL
Negative regulation of protein binding Source: ParkinsonsUK-UCL
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Negative regulation of Wnt-Frizzled-LRP5/6 complex assembly Source: ParkinsonsUK-UCL
Negative regulation of Wnt signaling pathway Source: UniProtKB
Positive regulation of cell death Source: ARUK-UCL
Positive regulation of gene expression Source: ARUK-UCL
Positive regulation of heart induction by negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
Positive regulation of JUN kinase activity Source: ARUK-UCL
Positive regulation of neuron death Source: ARUK-UCL
Positive regulation of tau-protein kinase activity Source: ARUK-UCL
Positive regulation of Wnt signaling pathway, calcium modulating pathway Source: ARUK-UCL
Positive regulation of Wnt signaling pathway, planar cell polarity pathway Source: ARUK-UCL
Regulation of dopaminergic neuron differentiation Source: ParkinsonsUK-UCL
Regulation of endodermal cell fate specification Source: BHF-UCL
Regulation of receptor internalization Source: BHF-UCL
Synapse pruning Source: ParkinsonsUK-UCL
Wnt signaling pathway Source: UniProtKB-KW

Cellular Location

Secreted

Zhu, G., Song, J., Chen, W., Yuan, D., Wang, W., Chen, X., ... & Zhu, J. (2021). Expression and role of Dickkopf-1 (Dkk1) in tumors: from the cells to the patients. Cancer Management and Research, 13, 659.

Supsavhad, W., Hassan, B. B., Simmons, J. K., Dirksen, W. P., Elshafae, S. M., Kohart, N. A., ... & Rosol, T. J. (2021). Effect of Dickkopf-1 (Dkk-1) and SP600125, a JNK Inhibitor, on Wnt Signaling in Canine Prostate Cancer Growth and Bone Metastases. Veterinary sciences, 8(8), 153.

Bahie, A., Abdalbary, M. M., El-Sayed, D. Y., Elzehery, R., El-Said, G., El-Kannishy, G., & Abd El Wahab, A. M. (2021). Relation of Wnt Signaling Pathway Inhibitors (Sclerostin and Dickkopf-1) to Left Ventricular Mass Index in Maintenance Hemodialysis Patients. International Journal of Nephrology, 2021.

Jaschke, N., Hofbauer, L. C., Göbel, A., & Rachner, T. D. (2020). Evolving functions of Dickkopf-1 in cancer and immunity. Cancer letters, 482, 1-7.

Jia, Y., Chen, L., Guo, S., & Li, Y. (2019). Baicalin induced colon cancer cells apoptosis through miR-217/DKK1-mediated inhibition of Wnt signaling pathway. Molecular biology reports, 46(2), 1693-1700.

Singh, A., Gupta, M. K., & Mishra, S. P. (2019). Study of correlation of level of expression of Wnt signaling pathway inhibitors sclerostin and dickkopf-1 with disease activity and severity in rheumatoid arthritis patients. Drug Discoveries & Therapeutics, 13(1), 22-27.

Lyros, O., Lamprecht, A. K., Nie, L., Thieme, R., Götzel, K., Gasparri, M., ... & Gockel, I. (2019). Dickkopf-1 (DKK1) promotes tumor growth via Akt-phosphorylation and independently of Wnt-axis in Barrett’s associated esophageal adenocarcinoma. American journal of cancer research, 9(2), 330.

Huang, Y., Liu, L., & Liu, A. (2018). Dickkopf-1: current knowledge and related diseases. Life sciences, 209, 249-254.

Kasoha, M., Bohle, R. M., Seibold, A., Gerlinger, C., Juhasz-Boess, I., & Solomayer, E. F. (2018). Dickkopf-1 (Dkk1) protein expression in breast cancer with special reference to bone metastases. Clinical & experimental metastasis, 35(8), 763-775.

Kagey, M. H., & He, X. (2017). Rationale for targeting the Wnt signalling modulator Dickkopf‐1 for oncology. British journal of pharmacology, 174(24), 4637-4650.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human DKK1 ELISA Kit (CAT#: V2LY-0626-LY1033)

Mouse Dkk1 ELISA Kit (CAT#: V2LY-0626-LY2282)

Human Recombinant DKK1 protein, hFc Tag (CAT#: V2LY-0526-LY3582)

Rhesus Recombinant DKK1 (256 Asn/Gln) protein, His Tag (CAT#: V2LY-0526-LY9703)

Mouse Recombinant DKK1 protein, His Tag (CAT#: V2LY-0526-LY8199)

Mouse Recombinant DKK1 protein, His Tag-1 (CAT#: V2LY-0526-LY8200)

Rat Recombinant DKK1 protein, His Tag (CAT#: V2LY-0526-LY9331)

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Mouse Recombinant DKK1 protein, His Tag (CAT#: V2LY-0526-LY8199)

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Rhesus Recombinant DKK1 (256 Asn/Gln) protein, His Tag (CAT#: V2LY-0526-LY9703)

Isotype control

For research use only. Not intended for any clinical use.

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