Human Recombinant EIF4EBP1 protein, His Tag-2 (V2LY-0526-LY1866)

Name: Human Recombinant EIF4EBP1 protein, His Tag-2
SKU: V2LY-0526-LY1866
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant EIF4EBP1 protein, His Tag consist of Amino Acid: 2-118 and predicts a molecular mass of 13.4 kDa.

Molecule Mass

13.4 kDa

Verified

HPLC

Sequence

Amino Acid: 2-118

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Eukaryotic Translation Initiation Factor 4E Binding Protein 1

Research Area

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

Biological Process

Cellular response to hypoxia Source: Ensembl
G1/S transition of mitotic cell cycle Source: UniProtKB
Lung development Source: Ensembl
Negative regulation of protein-containing complex assembly Source: Ensembl
Negative regulation of translational initiation Source: UniProtKB
Positive regulation of mitotic cell cycle Source: UniProtKB
Response to amino acid starvation Source: Ensembl
Response to ethanol Source: Ensembl
Response to ischemia Source: Ensembl
TOR signaling Source: UniProtKB

Cellular Location

Cytosol; Postsynaptic cytosol; Nucleus; Cytoplasm; Glutamatergic synapse; Protein-containing complex

PTM

Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70, corresponding to the hyperphosphorylated form, is regulated by mTORC1 and abolishes binding to EIF4E.
Ubiquitinated: when eIF4E levels are low, hypophosphorylated form is ubiquitinated by the BCR(KLHL25) complex, leading to its degradation and serving as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. Not ubiquitinated when hyperphosphorylated (at Thr-37, Thr-46, Ser-65 and Thr-70) or associated with eIF4E.

Hauffe, L., Picard, D., Musa, J., Remke, M., Grünewald, T. G., Rotblat, B., ... & Leprivier, G. (2022). Eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1) expression in glioblastoma is driven by ETS1-and MYBL2-dependent transcriptional activation. Cell death discovery, 8(1), 1-11.

Voeltzke, K., Scharov, K., Funk, C. M., Kahler, A., Picard, D., Hauffe, L., ... & Leprivier, G. (2022). EIF4EBP1 is transcriptionally upregulated by MYCN and associates with poor prognosis in neuroblastoma. Cell death discovery, 8(1), 1-12.

Zhang, L., & Su, X. (2022). Bioactive peptide inhibits acute myeloid leukemia cell proliferation by downregulating ALKBH5-mediated m6A demethylation of EIF4EBP1 and MLST8 mRNA. Cellular Oncology, 1-11.

Li, X., Zhang, Y., Su, L., Cai, L., Zhang, C., Zhang, J., ... & Huang, X. (2022). FGF21 alleviates pulmonary hypertension by inhibiting mTORC1/EIF4EBP1 pathway via H19. Journal of Cellular and Molecular Medicine, 26(10), 3005-3021.

Gui, C. P., Liao, B., Luo, C. G., Chen, Y. H., Tan, L., Tang, Y. M., ... & Wei, J. H. (2021). circCHST15 is a novel prognostic biomarker that promotes clear cell renal cell carcinoma cell proliferation and metastasis through the miR-125a-5p/EIF4EBP1 axis. Molecular Cancer, 20(1), 1-21.

Jiang, Y., Wu, S. Y., Chen, Y. L., Zhang, Z. M., Tao, Y. F., Xie, Y., ... & Lu, J. (2021). CEBPG promotes acute myeloid leukemia progression by enhancing EIF4EBP1. Cancer Cell International, 21(1), 1-12.

Yang, M., Yang, B., & Deng, D. (2021). Targeting of EIF4EBP1 by miR‐99a‐3p affects the functions of B lymphocytes via autophagy and aggravates SLE disease progression. Journal of cellular and molecular medicine, 25(21), 10291-10305.

Wan, P., Chen, Z., Zhong, W., Jiang, H., Huang, Z., Peng, D., ... & Chen, N. (2020). BRDT is a novel regulator of eIF4EBP1 in renal cell carcinoma. Oncology Reports, 44(6), 2475-2486.

Akbarian, F., Tabatabaiefar, M. A., Shaygannejad, V., Shahpouri, M. M., Badihian, N., Sajjadi, R., ... & Noori-Daloii, M. R. (2020). Upregulation of MTOR, RPS6KB1, and EIF4EBP1 in the whole blood samples of Iranian patients with multiple sclerosis compared to healthy controls. Metabolic brain disease, 35(8), 1309-1316.

Li, S. Q., Feng, J., Yang, M., Ai, X. P., He, M., & Liu, F. (2020). Sauchinone: a prospective therapeutic agent-mediated EIF4EBP1 down-regulation suppresses proliferation, invasion and migration of lung adenocarcinoma cells. Journal of natural medicines, 74(4), 777-787.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human EIF4EBP1 ELISA Kit (CAT#: V2LY-0626-LY4566)

Human Recombinant EIF4EBP1 protein, GST Tag (CAT#: V2LY-0526-LY1863)

Human Recombinant EIF4EBP1 protein, His Tag (CAT#: V2LY-0526-LY1864)

Human Recombinant EIF4EBP1 protein, His Tag-1 (CAT#: V2LY-0526-LY1865)

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Isotype control

For research use only. Not intended for any clinical use.

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