Human Recombinant ENSA protein, His Tag (V2LY-0526-LY3845)

Name: Human Recombinant ENSA protein, His Tag
SKU: V2LY-0526-LY3845
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ENSA protein, His Tag consist of Amino Acid: 1-121 and predicts a molecular mass of 15.2 kDa.

Molecule Mass

15.2 kDa

Sequence

Amino Acid: 1-121

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Endosulfine Alpha

Research Area

Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity).

Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.

Biological Process

Cell division Source: UniProtKB-KW
G2/M transition of mitotic cell cycle Source: UniProtKB
Mitotic cell cycle Source: UniProtKB
Negative regulation of protein dephosphorylation Source: GO_Central
Regulation of insulin secretion Source: MGI
Response to glucose Source: Ensembl
Response to nutrient Source: ProtInc

Cellular Location

Cytoplasm

PTM

Phosphorylation at Ser-67 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A (By similarity). Phosphorylated by PKA.

Watamura, N., Kakiya, N., Nilsson, P., Tsubuki, S., Kamano, N., Takahashi, M., ... & Saido, T. C. (2022). Somatostatin-evoked Aβ catabolism in the brain: mechanistic involvement of α-endosulfine-KATP channel pathway. Molecular psychiatry, 27(3), 1816-1828.

Chen, Y. Y., Ge, J. Y., Zhu, S. Y., Shao, Z. M., & Yu, K. D. (2022). Copy number amplification of ENSA promotes the progression of triple-negative breast cancer via cholesterol biosynthesis. Nature communications, 13(1), 1-16.

Thapa, C., Roivas, P., Haataja, T., Permi, P., & Pentikäinen, U. (2022). Interaction mechanism of endogenous PP2A inhibitor protein ENSA with PP2A. The FEBS Journal, 289(2), 519-534.

Watamura, N., Kakiya, N., Nilsson, P., Tsubuki, S., Kamano, N., Takahashi, M., ... & Saido, T. C. (2020). α-Endosulfine regulates amyloid β 42 via the modulation of neprilysin activity. bioRxiv.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant ENSA protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY3846)

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Human Recombinant ENSA protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY3846)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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