Human Recombinant EPDR1 protein, His Tag (V2LY-0526-LY3868)

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Basic Information

Expressed Host
HEK293 Cells
Protein Species
Human
Tag
His Tag
Protein Construction
This product is Human Recombinant EPDR1 protein, His Tag consist of Amino Acid: 1-224 and predicts a molecular mass of 23.2 kDa.
Molecule Mass
23.2 kDa
Sequence
Amino Acid: 1-224
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>95% as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile PBS
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
Ependymin Related 1
Research Area
Binds anionic lipids and gangliosides at acidic pH.
Biological Process
Cell-matrix adhesion Source: InterPro
Myofibroblast contraction Source: UniProtKB
Cellular Location
Secreted; Lysosome lumen. Lysosomal and also secreted.
PTM
N-glycosylated; the glycan contains mannose-6-phosphate moieties.By similarity

Ahmed, T., Flores, P. C., Pan, C. C., Ortiz, H. R., Lee, Y. S., Langlais, P. R., ... & Lee, N. Y. (2022). EPDR1 is a noncanonical effector of insulin-mediated angiogenesis regulated by an endothelial-specific TGF-β receptor complex. Journal of Biological Chemistry, 298(9).

El-Masry, O. S., Alamri, A. M., Alzahrani, F., & Alsamman, K. (2022). ADAMTS14, ARHGAP22, and EPDR1 as potential novel targets in acute myeloid leukaemia. Heliyon, 8(3), e09065.

Yang, Y., Zhang, H., Liu, Z., Zhao, F., & Liang, G. (2021). EPDR1 is related to stages and metastasize in bladder cancer and can be used as a prognostic biomarker. BMC urology, 21(1), 1-12.

Liu, X., Liu, C., Zhang, A., Wang, Q., Ge, J., Li, Q., & Xiao, J. (2021). Long non-coding RNA SDCBP2-AS1 delays the progression of ovarian cancer via microRNA-100-5p-targeted EPDR1. World Journal of Surgical Oncology, 19(1), 1-9.

Zhao, Z., Wang, Z., Wang, P., Liu, S., Li, Y., & Yang, X. (2021). EPDR1, Which Is Negatively Regulated by miR-429, Suppresses Epithelial Ovarian Cancer Progression via PI3K/AKT Signaling Pathway. Frontiers in oncology, 11, 751567-751567.

Gimeno-Valiente, F., Riffo-Campos, Á. L., Ayala, G., Tarazona, N., Gambardella, V., Rodríguez, F. M., ... & Castillo, J. (2020). EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness. Scientific reports, 10(1), 1-14.

Chen, R., & Zhang, Y. (2020). EPDR1 correlates with immune cell infiltration in hepatocellular carcinoma and can be used as a prognostic biomarker. Journal of cellular and molecular medicine, 24(20), 12107-12118.

Deshmukh, A. S., Peijs, L., Beaudry, J. L., Jespersen, N. Z., Nielsen, C. H., Ma, T., ... & Scheele, C. (2019). Proteomics-based comparative mapping of the secretomes of human brown and white adipocytes reveals EPDR1 as a novel batokine. Cell metabolism, 30(5), 963-975.

Wei, Y., Xiong, Z. J., Li, J., Zou, C., Cairo, C. W., Klassen, J. S., & Privé, G. G. (2019). Crystal structures of human lysosomal EPDR1 reveal homology with the superfamily of bacterial lipoprotein transporters. Communications biology, 2(1), 1-13.

Chu, C. H., Chang, S. C., Wang, H. H., Yang, S. H., Lai, K. C., & Lee, T. C. (2018). Prognostic values of EPDR1 hypermethylation and its inhibitory function on tumor invasion in colorectal cancer. Cancers, 10(10), 393.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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